3 research outputs found
Prevalence of Antiretroviral Drug Resistance Mutations and HIV-1 Subtypes among Newly-diagnosed Drugna\uefve Persons Visiting a Voluntary Testing and Counselling Centre in Northeastern South Africa
Data on antiretroviral drug resistance among drug-na\uefve persons
are important in developing sentinel surveillance policies. This study
was conducted to determine the prevalence of antiretroviral drug
resistance mutations among drug-na\uefve HIV-infected individuals
attending a voluntary testing and counselling centre at the Mankweng
Hospital in northeastern South Africa. In total, 79 drug-na\uefve
HIV-positive individuals were sequentially recruited during February
2008-December 2008. Drug resistance mutations were determined using the
calibrated population resistance tool available on the Stanford HIV
drug resistance database. Viral DNA was obtained from 57 (72%) of the
79 individuals. Reliable nucleotide sequences were obtained for 54
reverse transcriptase (RT) and 54 protease (PR) gene regions from 54
individuals. Overall, five sequences (9.3%) harboured drug resistance
mutations (95% confidence interval -1.53 to 16.99). Four (7.4%) of
these were nucleoside RT inhibitor mutations (D67G, D67E, T69D, and
T215Y), and one (1.9%) was a PR inhibitor mutation (M46I). No major
non-nucleoside RT resistance mutation was detected. Several minor
resistance mutations and polymorphisms common in subtype C viruses were
observed in the PR and RT genes. Phylogenetic analysis of the partial
pol sequences showed that 52 (96%) of the 54 isolates were HIV-1
subtype C. One isolate (08MB08ZA) was HIV-1 subtype B while another
(08MB26ZA) was related to HIV-1 subtype J. HIV-1 subtype recombination
analysis with REGA assigned the pol sequence to HIV subtype J (11_cpx)
with a bootstrap value of 75%. The prevalence of drug resistance
mutations observed in the population studied was relatively higher than
previously reported from other parts of South Africa. In addition, this
is apparently the first report of an HIV-1 subtype J-like virus from
northeastern South Africa
Prevalence of Antiretroviral Drug Resistance Mutations and HIV-1 Subtypes among Newly-diagnosed Drugnaïve Persons Visiting a Voluntary Testing and Counselling Centre in Northeastern South Africa
Data on antiretroviral drug resistance among drug-naïve persons
are important in developing sentinel surveillance policies. This study
was conducted to determine the prevalence of antiretroviral drug
resistance mutations among drug-naïve HIV-infected individuals
attending a voluntary testing and counselling centre at the Mankweng
Hospital in northeastern South Africa. In total, 79 drug-naïve
HIV-positive individuals were sequentially recruited during February
2008-December 2008. Drug resistance mutations were determined using the
calibrated population resistance tool available on the Stanford HIV
drug resistance database. Viral DNA was obtained from 57 (72%) of the
79 individuals. Reliable nucleotide sequences were obtained for 54
reverse transcriptase (RT) and 54 protease (PR) gene regions from 54
individuals. Overall, five sequences (9.3%) harboured drug resistance
mutations (95% confidence interval -1.53 to 16.99). Four (7.4%) of
these were nucleoside RT inhibitor mutations (D67G, D67E, T69D, and
T215Y), and one (1.9%) was a PR inhibitor mutation (M46I). No major
non-nucleoside RT resistance mutation was detected. Several minor
resistance mutations and polymorphisms common in subtype C viruses were
observed in the PR and RT genes. Phylogenetic analysis of the partial
pol sequences showed that 52 (96%) of the 54 isolates were HIV-1
subtype C. One isolate (08MB08ZA) was HIV-1 subtype B while another
(08MB26ZA) was related to HIV-1 subtype J. HIV-1 subtype recombination
analysis with REGA assigned the pol sequence to HIV subtype J (11_cpx)
with a bootstrap value of 75%. The prevalence of drug resistance
mutations observed in the population studied was relatively higher than
previously reported from other parts of South Africa. In addition, this
is apparently the first report of an HIV-1 subtype J-like virus from
northeastern South Africa