3 research outputs found
Stereodivergent Synthesis and Configurational Assignment of the C1–C15 Segment of Amphirionin‑5
The relative configuration
of the C3–C12 portion of amphirionin-5,
a novel marine polyketide with potent cell proliferation-promoting
activity, was established by the stereodivergent synthesis of six
diastereomeric model compounds and comparison of their NMR spectroscopic
data with those reported for the natural product. This study led to
the elucidation of the relative configuration between C4/C5 and C9/C12
and to the reassignment of the proposed configuration of the C9 position
of amphirionin-5
Direct and Stereospecific Interaction of Amphidinol 3 with Sterol in Lipid Bilayers
Amphidinol
3 (AM3), a polyhydroxy-polyene metabolite from the dinoflagellate Amphidinium klebsii, possesses potent antifungal
activity. Although AM3 permeabilizes phospholipid membranes only in
the presence of sterol, the detailed molecular basis by which AM3
recognizes sterols in membranes remains unknown. Here, we investigated
the molecular interaction between sterols and AM3 in membranes from
the viewpoint of stereospecific molecular recognition using ergosterol,
cholesterol, and epicholesterol, which is the 3-OH epimer of cholesterol.
Dye leakage assays, surface plasmon resonance experiments, <sup>2</sup>H and <sup>31</sup>P NMR measurements, and microscopic observations
revealed that AM3 directly interacts with membrane sterols through
the strict molecular recognition of the stereochemistry of the sterol
3-OH group. The direct interaction enhances the membrane binding efficiency
of AM3, which subsequently permeabilizes membranes without altering
membrane integrity
Amphirionin‑4 with Potent Proliferation-Promoting Activity on Bone Marrow Stromal Cells from a Marine Dinoflagellate <i>Amphidinium</i> Species
A linear
polyketide, amphirionin-4 (<b>1</b>), has been isolated
from cultivated algal cells of the marine dinoflagellate <i>Amphidinium</i> species. The structure was elucidated on the basis of detailed analyses
of 1D and 2D NMR data, and the absolute configurations of C-4 and
C-8 were determined using the modified Mosher’s method. Amphirionin-4
(<b>1</b>) exhibited extremely potent proliferation-promoting
activity on murine bone marrow stromal ST-2 cells (950% promotion)
at a concentration of 0.1 ng/mL