IBS subtype and CRH-BP SNP.

<p>We analyzed the associations between the SNPs and psychometric scores according to IBS subtype. (a) In individuals with diarrhea-type IBS, <i>rs10474485</i> A allele non-carriers showed higher scores than carriers. There were significant differences in the PSS (p = 0.018) and Trait (p = 0.017) scores. (b) In addition, in the IBS group with mixed symptoms, a significant difference was observed in the SDS score (p = 0.030). There was no significant difference in the constipation group. *p <i><</i> 0.05.</p

Sample Characteristics.

<p>Sample Characteristics.</p

Psychometric Scores.

<p>Psychometric Scores.</p

Sex differences in psychometric scores according to the CRH-BP SNP.

<p>(a) In analysis of male subjects (IBS + control), there was no significant difference (SDS, p = 0.864; PSS, p = 0.177; State, p = 0.979; Trait; p = 0.976). (b) In contrast, female A allele non-carriers of <i>rs10474485</i> showed significantly higher PSS (p = 0.028) and State of STAI (p = 0.035) scores and tended to have higher SDS (p = 0.083) and Trait of STAI (p = 0.078) scores than carriers. *p < 0.05.</p

Self-rating Depression Scale and the CRH-BP SNP.

<p>We analyzed associations between the selected SNP (<i>rs10474485</i>) and psychometric scores according to sex. (a) In male subjects, a significant group (IBS/control) × rs10474485 genotype interaction (p = 0.045) was observed. (b) Further, there was a significant group (IBS/control) × <i>rs10474485</i> A allele interaction (p = 0.017). *p <i><</i> 0.05.</p

Genotype Frequencies in the IBS Patients and Controls.

<p>Genotype Frequencies in the IBS Patients and Controls.</p

Self-rating Depression Scale and <i>CRH-R1</i> SNPs.

<p>Two-way ANOVA showed that IBS patients with <i>rs7029436</i> (<i>P</i> = 0.009) (right) and rs242924 (<i>P</i> = 0.013) (left) had significantly higher depression scale scores than controls with the same genotypes. However, there was no gene–group interaction.</p

Difference in bowel patterns between allele of <i>CRH-R1</i> SNPs.

<p>Each panel indicates bowel patterns (normal, constipation, mixed, or diarrhea) in the SNPs of (<b>A</b>) <i>rs7029436</i> (C+ vs C−, T+ vs T−), (<b>B</b>) <i>rs242924</i> (G+ vs G−, T+ vs T−), and (<b>C</b>) <i>rs110402</i> (G+ vs G−, A+ vs A−) and (<b>D</b>) TAT haplocopies (0, 1, or 2 copies). Significant differences in bowel patterns between the T alleles of <i>rs7029436</i> (<i>P</i> = 0.008), the T alleles of <i>rs242924</i> (<i>P</i> = 0.02), the A alleles of <i>rs110402</i> (<i>P</i> = 0.047), and among TAT haplocopies (<i>P</i> = 0.048, χ²-test) of <i>CRH-R1</i> SNPs were observed.</p

IBS subtype (bowel pattern) in relation to TAT haplotype copy number and sex.

<p>IBS subtype: C, constipation; M, mixed; D, diarrhea.</p

Difference in genotype of <i>CRH-R1</i> SNPs between controls and IBS patients.

<p>The SNPs <i>rs7029436</i> (<b>A</b>), <i>rs242924</i> (<b>B</b>), and <i>rs110402</i> (<b>C</b>), and TAT haplotype (<b>D</b>) were shown. The SNPs <i>rs7029436</i> (<i>P</i> = 0.013) and <i>rs242924</i> (<i>P</i> = 0.022, χ²-test) in IBS patients significantly differed from those in controls.</p