Optimization and Validation of a New Capillary Electrophoresis Method with Conductivity Detection for Determination of Small Anions in Red Wines

A capillary electrophoresis (CE) method has been developed and validated for determination of organic acids (oxalate, tartrate, malate, malonate, pyruvate, succinate, acetate, citrate, and lactate) and inorganic anions (sulfate and phosphate) in red wines. The separations were carried out in an automated separation system equipped with wide-bore (300 μm i.d.) fluoroplastic capillary and contact conductivity detector used for monitoring the separation and quantification of the analytes. The fast method (analysis time less than 5 min.) provided a good linearity of calibration curves (R2 > 0.9920) for the studied acids, as well as a good reproducibility of migration times (RSD < 1.5%). In total, 17 red wines were analyzed with the proposed method, including Vranec, Cabernet Sauvignon, and Merlot wines from various geographic areas (Demir Kapija, Kavadarci, Negotino, and Veles) in Macedonia. The used fully automated separation system (sample dilution not included) predetermined the developed CE method for routine analysis

Optimization and validation of capillary electrophoresis method for small-anions measurement in red wines

A capillary electrophoresis (CE) method has been developed and validated for analysis of organic acids (oxalate, tartrate, malate, malonate, pyruvate, succinate, acetate, citrate and lactate) and inorganic anions (sulfate and phosphate) in red wines. The separations were carried out in an automated separation system equipped with wide-bore (300 μm i.d.) fluoroplastic capillary with suppressed hydrodynamic and electroosmotic flow. Contact conductivity detection was used for monitoring of the separation and quantification of the analytes. Composition of carrier electrolyte was optimized to achieve sufficient resolution of organic and inorganic acids and adequate sensitivity of conductivity detection. The fast method (analysis time less than 5 min.) provided a good linearity of calibration curves (R 2 > 0.992) for the studied concentration range of the acids, as well as good reproducibility of migration times (RSD < 1.5 %). The used fully automated separation system (sample dilution not included) predetermined the developed CE method for routine analysis. In total 33 red wines were analysed with the proposed method, including 22 Macedonian red wines from various varieties and geographic areas as well as 11 commercial red wines from different countries