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Additional file 8: Table S6. Number of settled/treated larvae of each batch in DEAB 100, 300 ¾M or DMSO treatment

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Additional file 3: Table S1. Accession numbers of the gene used for construction of phylogenic tree. Amino acid sequences to construct tree were obtained from Uniprot or Echinobase ( http://www.echinobase.org/Echinobase/ ), Genbank

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Additional file 1: Figure S1. Phylogenic tree of Raldh (= Aldh1a, Aldehyde hydrogenase 1 alfa) and Aldh2 (Aldehyde hydrogenase 2) subfamily. The phylogenic tree was constructed by RAxML. raldhs of starfish made sister grouping with hemichordate aldh1as. Numbers at node show bootstrap value. The selected amino acid substation model was LG + F + G. The process to construct was described in material and method and the set of sequences is provided in Additional file 10: Supplementary dataset 1. Abbreviations of species were referred following, Hs; Homo sapiens (Human), Mm; Mus musculus (Mouse), Xt; Xenopus tropicalis (Western clawed frog), Dr; Danio rerio (Zebrafish), Bf; Branchiostoma floridae (Amphioxus), Ci; Ciona intestinalis (Transparent sea squirt), Sk; Saccoglossus kowalevskii (Acorn worm), Sp; Strongylocentrotus purpuratus (Purple sea urchin), Pp; Patiria pectinifera

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Additional file 10: Supplementary dataset 1. The dataset for phylogenetic analysis of Raldh and Aldh2

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Additional file 6: Table S4. Number of metamorphosed/settled larvae of each batch in RO 1 ¾M or DMSO treatment

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Additional file 4: Table S2. Sequences of primer for amplification of raldha, raldhb, raldhc, rar and rxr. We used 40-bp reverse primers including a 20-bp T3 promoter sequence to synthesize Dig-labelled RNA probes for in situ hybridization. Bold characters mean consensus sequence for T3 promoter

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Additional file 2: Figure S2. Phylogenic tree of RAR, RXR and THR (Thyroid hormone receptor). The phylogenic tree was constructed by RAxML. The selected amino acid substation model was LG + F + G. The set of sequences is provided in Additional file 11: Supplementary dataset 2. Abbreviations of species were referred following, Hs; Homo sapiens (Human), Mm; Mus musculus (Mouse), Xt; Xenopus tropicalis (Western clawed frog), Dr; Danio rerio (Zebrafish), Bf; Branchiostoma floridae (Amphioxus), Pm; Polyandrocarpa misakiensis (Tunicate), Bl; Branchiostoma lanceolatum (Amphioxus), Ci; Ciona intestinalis (Transparent sea squirt), Sk; Saccoglossus kowalevskii (Acorn worm), Sp; Strongylocentrotus purpuratus (Purple sea urchin), Pp; Patiria pectinifera, Dm; Drosophila melanogaster (Fruit fly), Rc; Reishia clavigera (Sea snail), Ls; Lymnaea stagnalis (Great pond snail), Tc; Tripedalia cystophora (Jellyfish)

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Additional file 11: Supplementary dataset 2. The dataset for phylogenetic analysis of RAR, RXR and THR

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Additional file 5: Table S3. Number of metamorphosed/treated larvae of each batch in RO + RA treatment experiment