MOESM1 of Oxygen-radical pretreatment promotes cellulose degradation by cellulolytic enzymes

Additional file 1: Table S1. The content of cellulose, hemicellulose, and lignin in non-pretreated, oxygen-gas-pretreated, and oxygen-radical-pretreated wheat straw. Figure S1. Effects of oxygen-radical pretreatment on MCC hydrolysis by cellulolytic enzymes in culture supernatant. Reducing sugars released from (a) oxygen-gas- or (b) oxygen-radical-pretreated MCC by enzymatic hydrolysis using culture supernatant were assayed using the DNS method. Error bars represent the mean ¹ standard error of the mean of three independent experiments. Figure S2. Gas chromatography spectra of the washing water of oxygen-gas- and oxygen-radical-pretreated wheat straw. (a) Oxygen-gas- and (b) oxygen-radical-pretreated wheat-straw samples were extracted with water to wash out enzyme inhibitors. Each treatment sample was washed with 25°C Milli-Q water, followed lyophilization, trimethylsilylation, and analysis of the liquid fraction by gas chromatography. Figure S3. Reducing-sugar production from washed and unwashed oxygen-radical-pretreated wheat straw. Reducing sugars released from washed and unwashed oxygen-radical-pretreated wheat straw after enzymatic hydrolysis using the supernatant from Phanerochaete chrysosporium cultures were assayed using the DNS method. Data are presented as the mean ¹ standard deviation of three experiments

MOESM1 of Oxygen-radical pretreatment promotes cellulose degradation by cellulolytic enzymes

Additional file 1: Table S1. The content of cellulose, hemicellulose, and lignin in non-pretreated, oxygen-gas-pretreated, and oxygen-radical-pretreated wheat straw. Figure S1. Effects of oxygen-radical pretreatment on MCC hydrolysis by cellulolytic enzymes in culture supernatant. Reducing sugars released from (a) oxygen-gas- or (b) oxygen-radical-pretreated MCC by enzymatic hydrolysis using culture supernatant were assayed using the DNS method. Error bars represent the mean ¹ standard error of the mean of three independent experiments. Figure S2. Gas chromatography spectra of the washing water of oxygen-gas- and oxygen-radical-pretreated wheat straw. (a) Oxygen-gas- and (b) oxygen-radical-pretreated wheat-straw samples were extracted with water to wash out enzyme inhibitors. Each treatment sample was washed with 25°C Milli-Q water, followed lyophilization, trimethylsilylation, and analysis of the liquid fraction by gas chromatography. Figure S3. Reducing-sugar production from washed and unwashed oxygen-radical-pretreated wheat straw. Reducing sugars released from washed and unwashed oxygen-radical-pretreated wheat straw after enzymatic hydrolysis using the supernatant from Phanerochaete chrysosporium cultures were assayed using the DNS method. Data are presented as the mean ¹ standard deviation of three experiments