Dissolution Dynamic Nuclear Polarization at Room Temperature Using Photoexcited Triplet Electrons

Dissolution dynamic nuclear polarization (DNP) has recently gained attention as a method to enhance the sensitivity of liquid NMR spectroscopy and MRI. We demonstrate dissolution of the sample hyperpolarized by DNP using photoexcited triplet electrons in 0.38 T at room temperature. The achieved polarization of 0.8% is 6100 times as high as that at thermal equilibrium under the condition. The result is an important step for DNP using photoexcited triplet electrons to become widely used in chemical and biomedical research

Amplification of partial ITS-1 region using specific primers.

<p>Primers AmerF3 and AmerR2 only amplified specific band of about 250-1, while no clear band was formed for types II and III. CongF and Nem5.8R resulted in formation of specific band of about 650 bp for types II and III, while prominent bands were not formed for type I.</p

Map of study site in Dzanga-Sangha Protected Areas, Central African Republic.

<p>Dark grey – Dzanga and Ndoki Sectors of the protected Dzanga Ndoki National Park; light grey - Dzanga Sangha Dense Forest Special Reserve; 1 – location of villages Bayanga, Mossapoula and Yandumbé; M – research camp Mongambe; B – research camp Bai Hokou.</p

Comparison of ITS-2 sequences of hookworms from apes and humans from Dzanga Sangha Protected Areas.

<p>Host and accession number in DNA database are given in parentheses. Dots indicate homologous nucleotides with <i>N. americanus</i> (<i>N. a.</i>) from Guatemala (AF217891); dash indicates absence of nucleotide. Major indels are shaded.</p

Comparison of ITS-1 sequences of hookworms from apes and humans from Dzanga Sangha Protected Areas.

<p>Host and accession number in DNA database are given in parentheses. Dots indicate homologous nucleotides with <i>N. americanus</i> (<i>N. a.</i>) from Guatemala (AF217891); dash indicates absence of nucleotide. Major indels are shaded.</p

Amino acid substitutions found among the haplotypes of partial <i>cox1</i> gene.

<p>* No. of haplotypes.</p

Restriction enzyme digestion of DNA sequences amplified with Civ18S1500F and Nem5.8R.

<p>By digestion with <i>Alu</i> I, two bands were formed for type I in area from 200 to 500 bp, while only one band was formed for types II and III. By digestion using <i>Hinc</i> II, two bands of about 300 and 500 bp were formed for type I, while only one band similar to original band was visible for types II and III.</p

Evolutionary relationships of <i>Necator</i> spp. from apes and humans in Dzanga-Sangha Protected Areas.

<p>Tree was reconstructed by Neighbor-Joining method on mtDNA <i>cox1</i> sequences each with 669 nucleotides. Host and accession number in DNA database in parentheses are given at each branch. Humans are CAR people except for two Europeans marked with asterisks, who acquired the infection in CAR. Bootstrap values larger than 50 are shown.</p

ESR and ¹H-,¹⁹F-ENDOR/TRIPLE Study of Fluorinated Diphenylnitroxides as Synthetic Bus Spin-Qubit Radicals with Client Qubits in Solution

Electron and nuclear spins as quantum bits (qubits) have been the focus of current issues in quantum information science/technology and related fields. From the viewpoint of chemistry, synthetic spin qubits are emerging. Diphenylnitroxide (DPNO) and its novel fluorine-substituted radicals are characterized as synthetic electron bus spin-qubits by continuous-wave ESR and ¹H-,¹⁹F-ENDOR/TRIPLE spectroscopy in solution and by DFT calculations. The partially fluorinated DPNOs have been synthesized to illustrate that they are candidates for the synthetic bus spin-qubits with well-defined client qubits. The fluorinated DPNOs undergo spin delocalization, dominating the robust spin polarization in the π-conjugation of phenyl rings, serving to increase the number of distinguishable client qubits from three to six