Localization of cerebral infarctions of the ameroid constrictor-implanted mice.

<p>Cx, cortex; CC, corpus callosum; CPu, caudoputamen; ACo, anterior commissure; F, Hippocampal fimbria; H, hippocampus.</p

Percentage of AC-implanted mice with cerebral infarctions.

<p>The histogram shows the number and percentage of AC-implanted mice (n = 8) that developed cerebral infarctions in cortex, corpus callosum, caudoputamen, anterior commissure, hippocampal fimbria, and hippocampus.</p

Cerebral blood flow (CBF) was gradually decreased in mice with the ameroid constrictors (ACs).

<p>(A) Representative CBF images of AC-implanted mice and bilateral common carotid artery stenosis (BCAS)-operated mice as assessed by laser speckle flowmetry at indicated time points. (B) Temporal profiles of CBF of the AC-implanted mice (n = 8) and BCAS-operated mice (n = 7) pre- and post-operation (2-way repeated-measures ANOVA, <i>p</i><0.05; unpaired t-test, #<i>p</i><0.01 vs. BCAS group). (C) Representative images of ACs at indicated time points.</p

Multiple cerebral infarcts in mice with ACs.

<p>Hematoxylin and eosin staining showed multiple infarcts in the cerebral cortex, the corpus callosum, the caudoputamen, the anterior commissure, the hippocampal fimbria, and the hippocampus of the mice in the AC group at 28-operation. Arrows indicate infarct changes. Scale bars indicate 200 µm.</p

Hippocampal neuronal loss in AC-implanted mice.

<p>Hematoxylin and eosin staining showed intact hippocampus of the BCAS-operated mouse (left panel) and hippocampal neuronal loss of the AC-implanted mouse (right panel) at 28 days post-operation. The inset shows a magnified image of the indicated area. Scale bar indicates 200 µm.</p

Survival rate in the AC group.

<p>(A) Kaplan-Meier method indicates survival rate of mice in the AC group (n = 29). (B) CBF images of the 4 non-surviving mice in the AC group which died during the second week. These mice died on the next day of last CBF measurement.</p

Half-maximal inhibitory concentration (IC₅₀, μM) for the binding of PIB to Aβ aggregates determined using ^99mTc-Ham complexes as ligands.

<p>Half-maximal inhibitory concentration (IC₅₀, μM) for the binding of PIB to Aβ aggregates determined using ^99mTc-Ham complexes as ligands.</p

Percent radiochemical purity of [^99mTc]MBT2 and [^99mTc]BT2 as a function of time.

<p>Percent radiochemical purity of [^99mTc]MBT2 and [^99mTc]BT2 as a function of time.</p

In vitro autoradiograms of human brain sections from a patient with CAA.

<p>The sections are labeled with [^99mTc]MBT2 (A) and [^99mTc]BT2B (C). The adjacent brain section is immunostained with an antibody against β-amyloid (B). ^aData from our previous study (Ref. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0163969#pone.0163969.ref028" target="_blank">28</a>).</p

In vitro autoradiograms of mouse brain sections labeled with ^99mTc-Ham complexes.

<p>The sections are from wild-type (A, E, and I) and Tg2576 (B, F, and J) mice. The same Tg2576 mouse brain sections are stained with thioflavin-S (C, G, and K). Blocking studies with PIB are also performed using the adjacent brain sections (D, H, and L). ^aData from our previous study (Ref. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0163969#pone.0163969.ref030" target="_blank">30</a>).</p