Effects of Common Fig ( Ficus carica

Formaldehyde (FA) is the leading cause of cellular injury and oxidative damage in testis that is one of the main infertility causes. There has been an increasing evidence of herbal remedies use in male infertility treatment. This assay examines the role of Ficus carica (Fc) leaf extracts in sperm parameters and testis of mice intoxicated with FA. Twenty-five adult male mice were randomly divided into control; sham; FA-treated (10 mg/kg twice per day); Fc-treated (200 mg/kg); and FA + Fc-treated groups. Cauda epididymal spermatozoa were analyzed for viability, count, and motility. Testes were weighed and gonadosomatic index (GSI) was calculated. Also, histoarchitecture of seminiferous tubules was assessed in the Haematoxylin and Eosin stained paraffin sections. The findings showed that FA significantly decreased GSI and increased percentage of immotile sperm compared with control group. Disorganized and vacuolated seminiferous epithelium, spermatogenic arrest, and lumen filled with immature germ cells were also observed in the testes. However, Fc leaf extracts improved sperm count, nonprogressive motility of spermatozoa, and GSI in FA-treated testes. Moreover, seminiferous tubule with spermatogenic arrest was rarely seen, indicating that Fc has the positive effects on testis and epididymal sperm parameters exposed with FA

Does in vitro application of pentoxifylline have beneficial effects in assisted male reproduction?

Abstract Application of nonspecific phosphodiesterases inhibitors, such as pentoxifylline (PTX), is a strategy utilised to aid sperm selection from immotile sperm samples prior to ICSI. No extensive studies have yet been performed to verify the safety of the clinical outcomes of ICSI after PTX administration. In this article, we summarise the data reported in the literature that assess the implication of in vitro usage of PTX on sperm parameters, as well as clinical outcomes during assisted male reproduction programme

<i>In vitro</i> cytotoxicity effects of date palm (<i>Phoenix dactylifera</i> L.) pollen on neonate mouse spermatogonial stem cells

<div><p>There is a fast growing tendency in the use of herbal remedies in developing countries. One of the traditional medicines used for male infertility treatment is date palm (<i>Phoenix dactylifera</i>) pollen (DPP). Isolated spermatogonial stem cells and sertoli cells using enzymatic digestion were grown in Dulbecco's modified Eagle's medium supplemented with 4% foetal bovine serum in the absence or presence of 0.06, 0.25 and 0.62 mg/mL concentrations of aqueous extract of DPP for 2 weeks. The assessment of mean number of the whole cells and the living cells showed that there were no significant differences between the mean viability percentage and proliferation rate between control and experimental groups (<i>P</i>>0.05). As there are no cytotoxicity effects of DPP in our cultural system, this system can be utilised for the enrichment or differentiation of these cells in clinical applications, cell replacement therapy, tissue regeneration and tissue engineering applications.</p></div

The effect of aqueous extract of Phoenix Dactylifera Pollen on In vitro viability and proliferation rate of neonatal mouse spermatogonial stem cells

Introduction: There is a fast growing tendency in the consumption of herbal remedies in the developing countries. One of the traditional medicines used for male infertility is Date palm (Phoenix dactylifera) pollen (DPP). The goal of the present study was to investigate the effect of aqueous extract of DPP on In vitro viability and proliferation rate of neonate mouse spermatogonial stem cells (SSCs). Methods: cell suspension includes sertoli cells and SSCs were isolated from neonatal 6 day-old mice testes by 2 steps enzymatic digestion. The cell suspension was cultured in DMEM and FCS 4% in the absence or presence of 0.06, 0.25 and 0.62 mg/ml of aqueous extract of DPP for 2 weeks. In order to evaluate the rate of SSCs expansion at the end of culture, the mean number of whole cells and living cells were considered as proliferation and survival rates respectively. Data analysis was done with ANOVA test. The significancy of the data was analyzed using ANOVA and Tukey post test. Results: The results showed that there were no significant differences between the mean percent of viability and proliferation rate between control and 0.06, 0.25 and 0.62 mg/ml of DPP-treated groups (P> 0.05). Conclusion: Our study showed that treatment of neonatal mouse testicular cell suspension with DPP had no toxic effects on viability percent and proliferation rate of these cells. Thus, we can use DPP for evaluate the in vitro pattern of SSCs colonization in the future studies