Comparative analysis of cancer genes in the human and chimpanzee genomes-0

<p><b>Copyright information:</b></p><p>Taken from "Comparative analysis of cancer genes in the human and chimpanzee genomes"</p><p>BMC Genomics 2006;7():15-15.</p><p>Published online 26 Jan 2006</p><p>PMCID:PMC1382208.</p><p>Copyright © 2006 Puente et al; licensee BioMed Central Ltd.</p

Comparative analysis of cancer genes in the human and chimpanzee genomes-1

<p><b>Copyright information:</b></p><p>Taken from "Comparative analysis of cancer genes in the human and chimpanzee genomes"</p><p>BMC Genomics 2006;7():15-15.</p><p>Published online 26 Jan 2006</p><p>PMCID:PMC1382208.</p><p>Copyright © 2006 Puente et al; licensee BioMed Central Ltd.</p>e-rich domain; DB: DNA-binding domain; TD: tetramerization domain; and RD: C-terminal regulatory domain). The proline-rich domain containing Arg72 () was sequenced in different primate species and aligned to the human sequence

<i>Alu</i>-URA-<i>Alu</i> pRS415 (pAUA).

<p>A construct containing a <i>URA3</i> gene flanked by identical <i>AluSp</i> repetitive elements was inserted into the low-copy-number yeast plasmid pRS415.</p

<i>pfh1-L430P</i> does not complement wild-type <i>pfh1⁺</i> function.

<p>(<b>A</b>) Complementation assay in <i>pfh1::loxP pfh1⁺ kanMX6 loxP</i> strain with either the L430P mutation (<i>leu::pfh1-L430P</i>), Pfh1 (<i>leu::pfh1⁺</i>), or an empty vector (<i>leu::leu⁺</i>) strain after transformation with a plasmid expressing Cre (<i>cre⁺</i>) or Cre-Y324F (<i>cre</i>⁻). The transformation plates are contrast-inverted pictures. (<b>B</b>) Anti-Pfh1 western blot of wild-type Pfh1GFP (WT) and three independent clones (1–3) expressing Pfh1-L430P. <i>S. pombe</i> cells were grown in the absence (−) or presence (+) of thiamine. 0, 12, and 24 hours indicates the amount of time cells were grown in the presence of thiamine. The loading control (LC) is a non-specific background band. (<b>C</b>) Heterologous expression constructs of <i>pfh1-nuc</i> or <i>pfh1-mt*</i> are indicated in the presence of <i>pfh1::loxP pfh1⁺ kanMX6 loxP</i> and <i>leu::pfh1-L430P</i>. Contrast-inverted pictures of transformation plates with <i>cre⁺</i> on the top and <i>cre⁻</i> on the bottom.</p