La Petite presse : journal quotidien... / [rédacteur en chef : Balathier Bragelonne]

10 décembre 18691869/12/10 (A4,N1331)

Additional file 3: Table S3. of A single-nucleotide-polymorphism real-time PCR assay for genotyping of Mycobacterium tuberculosis complex in peri-urban Kampala

LSP-PCR and LRPS sample analyses. (DOC 43 kb

Magnitude of <i>Mycobacterium tuberculosis</i> (Mtb) specific T cell responses in smear and culture positive, HIV negative, pulmonary TB patients over time during antituberculosis therapy.

<p>The magnitude of the Mtb specific CD4⁺ T cell response (A) and Mtb specific CD8⁺ T cell response (B) is shown by IFN-γ ELISPOT in response to ESAT-6 and CFP-10 and reported in spot forming units per 250,000 T cells. Evaluable samples (n) analyzed per time point were 49, 48 and 42 for baseline, week 8 and week 24 respectively. A negative binomial mixed model was used to assess the overall change during 24 weeks. Raw data, surrounded by the box demonstrates the distribution of 25–75% of the data, with the whiskers representing the non-outlier data, and the horizontal line depicting the median. Raw data is shown with a triangle, outliers with a circle, and extremes with a star.</p

Univariate and multivariate analyses of predictors of the Mtb specific T cell response among HIV negative, smear positive, culture confirmed pulmonary TB patients undergoing antituberculosis treatment.

*<p>BMI is included in the multivariate model with treatment duration.</p>†<p>The association between variables and the Mtb specific T cell response was analyzed at the baseline time point.</p>‡<p>Statistical method: a negative binomial mixed model.</p>§<p>AFB smear grade 0 (smear negative) to 4+.</p>||<p>Extent: limited or moderate versus extensive by chest radiograph.</p>**<p>Cavitation: ≤ 4 cm versus >4 cm.</p><p>n/a. not applicable.</p

<i>Mycobacterium tuberculosis</i> (Mtb) specific T cell responses during antituberculosis treatment in subjects with baseline malnutrition (BMI≤17).

<p>The subgroup of subjects, who started therapy with a BMI≤17 with baseline and week 24 analyzable ELISPOT data (n = 11) are shown. The magnitude of the Mtb specific T cell response is shown by IFN-γ ELISPOT to ESAT-6 and CFP-10 and reported in spot forming units per 250,000 T cells. Connected lines at baseline and week 24 reflect a individual subject's profile for the Mtb specific CD4⁺ T cell response (A) and the Mtb specific CD8⁺ T cell response (B).</p

Microbiologic burden of <i>mycobacterium tuberculosis</i> in relation to duration of treatment.

<p>Definition of abbreviations: col.  =  colonies</p>*<p>Total n (%) calculated less missing data and contamination</p

Quantitative cultures and treatment duration.

<p>Quantitative cultures from subjects with smear positive pulmonary TB as shown by the log of colony forming units (CFU) enumerated on 7H10 media at week 0 (n = 35) and week 4 (n = 14). Raw data, shown as a triangle, surrounded by the box demonstrates the distribution of 25–75% of the data, with the whiskers representing the non-outlier data, and the horizontal line depicting the median. The dotted line connects the median points. Wilcoxon signed rank test was performed comparing subjects with week 0 and week 4 (n = 12).</p

<i>Mycobacterium tuberculosis</i> (Mtb) specific T cell responses at baseline and BMI.

<p>The magnitude of the Mtb specific T cell response by IFN-γ ELISPOT to ESAT-6 and CFP-10, and reported in spot forming units/250,000 T cells, is shown at baseline for the Mtb specific CD4⁺ response (A) and the Mtb specific CD8⁺ response (B) for BMI≤17 (n = 14) and >17 (n = 34). A negative binomial model was used to assess differences in the Mtb specific T cell responses and BMI≤17 and >17. Raw data, shown as a triangle, surrounded by the box demonstrates the distribution of 25–75% of the data, with the whiskers representing the non-outlier data, and the horizontal line depicting the median.</p

Individual profiles of the absolute change in the Mtb specific T cell responses (spot forming units; SFU) from baseline to week 8 and baseline to week 24 in smear and culture positive, HIV negative, pulmonary TB patients during antituberculosis therapy.

<p>The waterfall plots show the absolute change in magnitude (SFU) between the baseline time point to week 8 for the Mtb specific CD4⁺ (A) and CD8⁺ (C) T cell response. The absolute change in magnitude for the baseline time point to week 24 is shown for the Mtb specific CD4⁺ (B) and CD8⁺ (D) T cell response. Black bars represent SFU decreasing from baseline to the respective time point, whereas white bars represent increasing SFU from baseline to the respective time point.</p