Additional file 1: of Effects of sex and DTNBP1 (dysbindin) null gene mutation on the developmental GluN2B-GluN2A switch in the mouse cortex and hippocampus

Supporting information. Figure S1. Synaptophysin abundance in membrane and PSD fractions from the frontal cortex across postnatal development. Figure S2. Representative images of blots used for quantification of NMDA receptor signaling proteins in the hippocampus. Figure S3. Loading standard curves for PSD fractions from the frontal cortex. Figure S4. Loading standard curves for membrane fractions from the frontal cortex. Figure S5. Loading standard curves for PSD fractions from the hippocampus, using P7 samples only. Figure S6. Loading standard curves for PSD fractions from the hippocampus, using P56 samples only. Figure S7. A–G Main effects of age, not described in main text, for proteins quantified in this study; H, I For SRC and PLCγ, distinct developmental trajectories were seen in different subcellular compartments, with stable SRC and PLCγ levels seen in PSD enrichments despite significantly decreasing levels in membranes in general. PSD- postsynaptic density, membranes- crude synaptic membranes (cSM), FCx- frontal cortex, HIP- hippocampus. *p < 0.05, **p < 0.005, ***p < 0.0005, ****p < 0.00005, *****p < 1 × 10−5. (DOCX 1627 kb