Supplementary Figure 12 from Peritumoral Immune-suppressive Mechanisms Impede Intratumoral Lymphocyte Infiltration into Colorectal Cancer Liver versus Lung Metastases

Clustered heatmap of relative expression of proteins (GeoMX DSP) per ROI from CRCmetastatic tumor specimens (5 tumors/metastatic site).</p

Supplementary Figure 10 from Peritumoral Immune-suppressive Mechanisms Impede Intratumoral Lymphocyte Infiltration into Colorectal Cancer Liver versus Lung Metastases

Distribution of LA and TLS across colorectal cancer sites.</p

Graphic Summary from Peritumoral Immune-suppressive Mechanisms Impede Intratumoral Lymphocyte Infiltration into Colorectal Cancer Liver versus Lung Metastases

Graphic Summary</p

FIGURE 6 from Peritumoral Immune-suppressive Mechanisms Impede Intratumoral Lymphocyte Infiltration into Colorectal Cancer Liver versus Lung Metastases

Analysis of immune cells between paired primary and liver metastases. A, Representative images of the tumor core from two individual patient's paired primary colorectal cancer and metastatic liver tumors. B, Correlations of CD4 and CD8 T cells in the paired primary and liver metastases. R2 were calculated with the density of immune cells in different histologic regions.</p

FIGURE 7 from Peritumoral Immune-suppressive Mechanisms Impede Intratumoral Lymphocyte Infiltration into Colorectal Cancer Liver versus Lung Metastases

Higher immune activity in lung metastasis, while more immune suppressive in the liver and peritoneal metastases. A, Representative image of ROI selection in GeoMx digital spatial profiling from a colorectal cancer lung metastasis. Cancer island and stroma within tumor core were identified by the expression of Pan-CK. Cell identity markers from metastatic colorectal cancer tumors (B) and markers highly expressing in the liver (C), lung (D), and peritoneal (E) metastases in GeoMx assay. Paired comparison of gene expression between each two organs in the different histologic regions shown in bottom row. F, GeoMx markers expressing in the LA. Statistical significance was determined by Wilcoxon signed-rank test for B–F.</p

Supplementary Figure 6 from Peritumoral Immune-suppressive Mechanisms Impede Intratumoral Lymphocyte Infiltration into Colorectal Cancer Liver versus Lung Metastases

Distribution of myeloid cells in the cancer island, stroma and necrosis tissue within tumor core and inner invasive region.</p

FIGURE 5 from Peritumoral Immune-suppressive Mechanisms Impede Intratumoral Lymphocyte Infiltration into Colorectal Cancer Liver versus Lung Metastases

Distribution of LA and TLS across colorectal cancer sites. A, Representative images of primary colorectal cancer tumor. Numbered insets show higher magnification. The second inset magnifies the same area seen in Fig. 1B. B, Representative images of primary and metastatic colorectal cancer tumors stained with mIF Panel 4. The boxes highlight the LA in the primary and metastatic tumors. C, Percentage of tumor specimens with intratumoral LA in primary and metastatic tumors. Statistical significance was determined by χ2 test. D, The number and percentage of area of LA in the metastatic tumor calculated in five individual compartments. E, Correlations of LA and various immune cells. PCC were calculated from the density LA and sporadic immune cells in different histologic regions from primary and metastatic colorectal cancer tumors from mIF Panel 4.</p

Supplementary Figure 2 from Peritumoral Immune-suppressive Mechanisms Impede Intratumoral Lymphocyte Infiltration into Colorectal Cancer Liver versus Lung Metastases

Characterization of immune cells in metastatic CRC TME by mIF.</p

Supplementary Figure 5 from Peritumoral Immune-suppressive Mechanisms Impede Intratumoral Lymphocyte Infiltration into Colorectal Cancer Liver versus Lung Metastases

Distribution of lymphocytes in the cancer island, stroma and necrosis tissue within tumorcore and inner invasive region.</p

FIGURE 3 from Peritumoral Immune-suppressive Mechanisms Impede Intratumoral Lymphocyte Infiltration into Colorectal Cancer Liver versus Lung Metastases

Distribution of myeloid cells in diverse tumor regions. A, Representative images of primary and metastatic colorectal cancer tumors stained with mIF panel 2, five images in each column were selected from the same tumor. B, Cell density (log10 cells/mm2) of myeloid cells and tumor cells in the TME in different histopathologic regions (Core, Inner, Outer, Juxta and Distal). Paired comparison of cell density between each two organs in the different histologic regions shown in bottom row. Statistical significance was determined by Wilcoxon signed-rank test. Cell density (log10 cells/mm2) of PD-L1–positive cells in TME (C) and percentage of PD-L1–positive cells within indicated cell types (D) in different histopathologic regions. Paired comparison between primary and metastatic tumor sites are in Supplementary Fig. S3D and S3E.</p