Associations of Autism Spectrum Quotient and Personality Profiles with Eating Behaviors in Patients with Anorexia Nervosa and in a Non-clinical Population

Article信州医学雑誌 67(3): 157-166(2019)journal articl

Five-point Likert scaling on MRI predicts clinically significant prostate carcinoma

Background: To clarify the relationship between the probability of prostate cancer scaled using a 5-point Likert system and the biological characteristics of corresponding tumor foci. Methods: The present study involved 44 patients undergoing 3.0-Tesla multiparametric MRI before laparoscopic radical prostatectomy. Tracing based on pathological and MRI findings was performed. The relationship between the probability of cancer scaled using the 5-point Likert system and the biological characteristics of corresponding tumor foci was evaluated. Results: A total of 102 tumor foci were identified histologically from the 44 specimens. Of the 102 tumors, 55 were assigned a score based on MRI findings (score 1: n = 3; score 2: n = 3; score 3: n = 16; score 4: n = 11 score 5: n = 22), while 47 were not pointed out on MRI. The tracing study revealed that the proportion of >0.5 cm3 tumors increased according to the upgrade of Likert scores (score 1 or 2: 33 %; score 3: 68.8 %; score 4 or 5: 90.9 %, χ2 test, p 7 also increased from scale 2 to scale 5 (scale 2: 0 %; scale 3: 56.3 %; scale 4: 72.7 %; 5: 90.9 %, χ2 test, p = 0.0001). On using score 3 or higher as the threshold of cancer detection on MRI, the detection rate markedly improved if the tumor volume exceeded 0.5 cm3 (<0.2 cm3: 10.3 %; 0.2-0.5 cm3: 25 %; 0.5-1.0 cm3: 66.7 %; 1.0 < cm3: 92.1 %). Conclusions: Each Likert scale favobably reflected the corresponding tumor’s volume and Gleason score. Our observations show that “score 3 or higher” could be a useful threshold to predict clinically significant carcinoma when considering treatment options

A 100%-complete sequence reveals unusually simple genomic features in the hot-spring red alga Cyanidioschyzon merolae

<p>Abstract</p> <p>Background</p> <p>All previously reported eukaryotic nuclear genome sequences have been incomplete, especially in highly repeated units and chromosomal ends. Because repetitive DNA is important for many aspects of biology, complete chromosomal structures are fundamental for understanding eukaryotic cells. Our earlier, nearly complete genome sequence of the hot-spring red alga <it>Cyanidioschyzon merolae </it>revealed several unique features, including just three ribosomal DNA copies, very few introns, and a small total number of genes. However, because the exact structures of certain functionally important repeated elements remained ambiguous, that sequence was not complete. Obviously, those ambiguities needed to be resolved before the unique features of the <it>C. merolae </it>genome could be summarized, and the ambiguities could only be resolved by completing the sequence. Therefore, we aimed to complete all previous gaps and sequence all remaining chromosomal ends, and now report the first nuclear-genome sequence for any eukaryote that is 100% complete.</p> <p>Results</p> <p>Our present complete sequence consists of 16546747 nucleotides covering 100% of the 20 linear chromosomes from telomere to telomere, representing the simple and unique chromosomal structures of the eukaryotic cell. We have unambiguously established that the <it>C. merolae </it>genome contains the smallest known histone-gene cluster, a unique telomeric repeat for all chromosomal ends, and an extremely low number of transposons.</p> <p>Conclusion</p> <p>By virtue of these attributes and others that we had discovered previously, <it>C. merolae </it>appears to have the simplest nuclear genome of the non-symbiotic eukaryotes. These unusually simple genomic features in the 100% complete genome sequence of <it>C. merolae </it>are extremely useful for further studies of eukaryotic cells.</p

Case of laryngeal venous malformations requiring repeated advanced airway management in the perioperative course

Abstract Laryngeal venous malformations rarely but do cause airway obstruction resulting in life‐threatening events. The perioperative airway management for the patients with them has not been well established. We suggest a strategy for laryngeal venous malformations management in the patients who undergo surgery in addition to planning for airway management

Sleep Quality and Nutrient Intake in Japanese Female University Student-Athletes: A Cross-Sectional Study

Objectives: This study aimed to characterize the sleep quality and nutrient intake of Japanese female college athletes to provide specific conditioning support. Methods: A cross-sectional survey conducted between December 2019 and January 2020 during the regular training season in Niigata City, located at 139&deg;02&prime; E longitude and 37&deg;55&prime; N latitude. Overall, 120 female university student-athletes from eight clubs were selected. All sports were at the national level of competition. The Pittsburgh Sleep Quality Index (PSQI) assessed sleep quality. Nutrient intake was assessed using Excel nutrition software through the Food Intake Frequency Questionnaire. Results: The mean PSQI score was 4.5 &plusmn; 2.5, with 29% of participants having a PSQI score &ge; 5.5. The sleep duration was 6.8 &plusmn; 1.1 h, with 45% of participants sleeping &lt;7 h. The energy intake was 1800 &plusmn; 419 kcal, with no correlation between PSQI score and nutrient intake. Conclusions: PSQI scores were higher compared with other studies, but many participants had shorter sleep duration and lower nutrient intake, these data suggest that there is the possibility of improving the habits of Japanese athletes by increasing the subjects&rsquo; amount of sleep time and food intake

Sleep Quality and Nutrient Intake in Japanese Female University Student-Athletes: A Cross-Sectional Study

Objectives: This study aimed to characterize the sleep quality and nutrient intake of Japanese female college athletes to provide specific conditioning support. Methods: A cross-sectional survey conducted between December 2019 and January 2020 during the regular training season in Niigata City, located at 139°02′ E longitude and 37°55′ N latitude. Overall, 120 female university student-athletes from eight clubs were selected. All sports were at the national level of competition. The Pittsburgh Sleep Quality Index (PSQI) assessed sleep quality. Nutrient intake was assessed using Excel nutrition software through the Food Intake Frequency Questionnaire. Results: The mean PSQI score was 4.5 ± 2.5, with 29% of participants having a PSQI score ≥ 5.5. The sleep duration was 6.8 ± 1.1 h, with 45% of participants sleeping <7 h. The energy intake was 1800 ± 419 kcal, with no correlation between PSQI score and nutrient intake. Conclusions: PSQI scores were higher compared with other studies, but many participants had shorter sleep duration and lower nutrient intake, these data suggest that there is the possibility of improving the habits of Japanese athletes by increasing the subjects’ amount of sleep time and food intake

Creation of haemoglobin A1c direct oxidase from fructosyl peptide oxidase by combined structure-based site specific mutagenesis and random mutagenesis

糖尿病マーカー、ヘモグロビンA1cを直接酸化できる酵素の創製 --シンプルかつ短時間測定が可能な新規測定試薬開発に大きく前進--. 京都大学プレスリリース. 2019-01-31.The currently available haemoglobin A1c (HbA1c) enzymatic assay consists of two specific steps: proteolysis of HbA1c and oxidation of the liberated fructosyl peptide by fructosyl peptide oxidase (FPOX). To develop a more convenient and high throughput assay, we devised novel protease-free assay system employing modified FPOX with HbA1c oxidation activity, namely HbA1c direct oxidase (HbA1cOX). AnFPOX-15, a modified FPOX from Aspergillus nidulans, was selected for conversion to HbA1cOX. As deduced from the crystal structure of AnFPOX-15, R61 was expected to obstruct the entrance of bulky substrates. An R61G mutant was thus constructed to open the gate at the active site. The prepared mutant exhibited significant reactivity for fructosyl hexapeptide (F-6P, N-terminal amino acids of HbA1c), and its crystal structure revealed a wider gate observed for AnFPOX-15. To improve the reactivity for F-6P, several mutagenesis approaches were performed. The ultimately generated AnFPOX-47 exhibited the highest F-6P reactivity and possessed HbA1c oxidation activity. HbA1c levels in blood samples as measured using the direct assay system using AnFPOX-47 were highly correlated with the levels measured using the conventional HPLC method. In this study, FPOX was successfully converted to HbA1cOX, which could represent a novel in vitro diagnostic modality for diabetes mellitus

A novel aldo–keto reductase gene is involved in 6′-deoxychalcone biosynthesis in dahlia (Dahlia variabilis)

The yellow pigments of dahlia flowers are derived from 6′-deoxychalcones, which are synthesized via a two-step process, involving the conversion of 3-malonyl-CoA and 4-coumaloyl-CoA into isoliquiritigenin in the first step, and the subsequent generation of butein from isoliquiritigenin. The first step reaction is catalyzed by chalcone synthase (CHS) and aldo–keto reductase (AKR). AKR has been implicated in the isoflavone biosynthesis in legumes, however, isolation of butein biosynthesis related AKR members are yet to be reported. A comparative RNA-seq analysis between two dahlia cultivars, ‘Shukuhai’ and its butein-deficient lateral mutant ‘Rinka’, was used in this study to identify a novel AKR gene involved in 6'-deoxychalcone biosynthesis. DvAKR1 encoded a AKR 13 sub-family protein with significant differential expression levels, and was phylogenetically distinct from the chalcone reductases, which belongs to the AKR 4A sub-family in legumes. DNA sequence variation and expression profiles of DvAKR1 gene were correlated with 6′-deoxychalcone accumulation in the tested dahlia cultivars. A single over-expression analysis of DvAKR1 was not sufficient to initiate the accumulation of isoliquiritigenin in tobacco, in contrast, its co-overexpression with a chalcone 4′-O-glucosyltransferase (Am4′CGT) from Antirrhinum majus and a MYB transcription factor, CaMYBA from Capsicum annuum successfully induced isoliquiritigenin accumulation. In addition, DvAKR1 homologous gene expression was detected in Coreopsideae species accumulating 6′-deoxychalcone, but not in Asteraceae species lacking 6′-deoxychalcone production. These results not only demonstrate the involvement of DvAKR1 in the biosynthesis of 6'-deoxychalcone in dahlia, but also show that 6′-deoxychalcone occurrence in Coreopsideae species developed evolutionarily independent from legume species