Predominant variable region gene usage by gamma/delta T cell receptor-bearing cells in the adult thymus.

Previous studies have indicated that the diversity of gamma genes expressed by gamma/delta-bearing murine T cells is limited, but comparable information concerning the expressed diversity of delta genes is lacking. In this study, we have investigated the rearrangement and expression of delta and gamma genes in T cell hybridomas that express gamma/delta T cell receptors. Three productive delta chain cDNA clones were isolated (delta 7.3, delta 7.1, and delta 2.3) that encode new variable region sequences. Two of the delta cDNAs differ significantly from those observed in the V alpha repertoire. In addition, one cDNA expressed a new J delta region (J delta 2), which was localized between J delta 1 and C delta genes. Using these and other delta gene probes and gamma gene probes, we found that five independent hybridomas expressed four different V delta s and three different V gamma s. However, analysis of an enriched population of gamma/delta-expressing cells from the adult thymus suggests that only a few V delta genes and one V gamma gene are used by the majority of the cells. These results suggest that important components of receptor chain that contribute to specificity (i.e., the germline V gene sequences) are relatively nondiverse in the thymic gamma/delta population

Drag forces on a bed particle in open-channel flow : Effects of pressure spatial fluctuations and very-large-scale motions

The study has been supported by two EPSRC/UK grants, ‘High-resolution numerical and experimental studies of turbulence-induced sediment erosion and near-bed transport’ (EP/G056404/1) and ‘Bed friction in rough-bed free-surface flows: a theoretical framework, roughness regimes, and quantification’ (EP/K041088/1). I.M. also acknowledges the support of the Australian Research Council.Peer reviewedPublisher PD

The Influence of Spatial Resolution due to Hot-Wire Sensors on Measurements in Wall-Bounded Turbulence.

Reassessment of compiled data reveal that recorded scatter in the hot-wire measured near-wall peak in viscous-scaled streamwise turbulence intensity is due in large part to the simultaneous competing effects of Reynolds number and viscous-scaled wire-length l ( lUt n, where l is the wirelength, Ut is friction velocity and n is kinematic viscosity). These competing factors can explain much of the disparity in existing literature, in particular explaining how previous studies have incorrectly concluded that the inner-scaled near-wall peak is independent of Re. We also investigate the appearance of the, so-called, ‘outerpeak’ in the broadband streamwise intensity, found by some researchers to occur within the log-region of high Reynolds number boundary layers. We show that this ‘outer-peak’ is most likely a symptom of attenuation of small-scales due to large l . Fully mapped energy spectra, obtained with two different l , are presented to demonstrate this phenomena. The spatial attenuation resulting from wires with large l effectively filters small-scale fluctuations from the recorded signal

Oil film interferometry in high Reynolds number turbulent boundary layers

There is continuing debate regarding the validity of skin friction measurements that are dependent on the functional form of the mean velocity profile, for example, the Clauser chart method. This has brought about the need for independent and direct measures of wall shear stress, tw. Of the independent methods to measure tw, oil film interferometry is the most promising, and it has been extensively used recently at low and moderately high Reynolds number. The technique uses interferometry to measure the thinning rate of an oil film, which is linearly related to the level of shear stress acting on the oil film. In this paper we report on the use of this technique in a high Reynolds number boundary layer up to Rq = 50,000. Being an independent measure of tw, the oil film measurement can be used as a means to validate more conventional techniques, such as the Preston tube and Clauser chart at these high Reynolds numbers. The oil-film measurement is validated by making comparative measurements of tw in a large-scale fully-developed channel flow facility where the skin friction is known from the pressure gradient along the channe

Continuous measurement of global difference coupling using a phase-locked-loop tune meter in the Relativistic Heavy Ion Collider

We present a new technique to continuously measure and compensate the global difference coupling coefficient through the continuous measurements of eigenmode projection parameters, using a high resolution phase-locked-loop tune meter. First, four eigenmode projection parameters are defined as the observables for weak difference coupling. Then, their analytical expressions are obtained using the strict matrix treatment and the Hamiltonian perturbation theory of linear coupling. From these parameters, the complex global coupling coefficient can be fully determined and compensated. This method was successfully demonstrated in the Relativistic Heavy Ion Collider (RHIC) 2006 run

‘The International Teacher Leadership project,’ a case of international action research.

Copyright CARNThe paper arises from the International Teacher Leadership project, a research and development project involving researchers and practitioners in 14 European countries. The paper provides a conceptual exploration of the idea of teacher leadership and its role in educational reform, central to which is the idea that teachers, regardless of their level of power and organisational position, can engage in the leadership of enquiry-based development activity aimed at influencing their colleagues and embedding improved practices in their schools. The paper provides an outline of the project’s methodology which builds on that used in the Carpe Vitam Leadership for Learning project (Frost, 2008a). It is a form of collaborative action research which is highly developmental and discursive. It seeks to identify principles, strategies and tools that can be applied in a range of cultural settings. The paper includes a thematic analysis of the cultural contexts and policy environments of the participating countries in order to identify the obstacles to teacher leadership and to inform the nature of the support strategies employed