Impact of calcium aluminate cement with additives on dental pulp-derived cells

Calcium aluminate cement (CAC) has been highlighted as a promising alternative for endodontic use aiming at periapical tissue repair. However, its effects on dental pulp cells have been poorly explored. Objective: This study assessed the impact of calcium chloride (CaCl2) and bismuth oxide (Bi2O3) or zinc oxide (ZnO) additives on odontoblast cell response to CAC. Methodology: MDPC-23 cells were exposed for up to 14 d: 1) CAC with 2.8% CaCl2 and 25% ZnO (CACz); 2) CAC with 2.8% CaCl2 and 25% Bi2O3 (CACb); 3) CAC with 10% CaCl2 and 25% Bi2O3 (CACb+); or 4) mineral trioxide aggregate (MTA), placed on inserts. Non-exposed cultures served as control. Cell morphology, cell viability, gene expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), and dentin matrix protein 1 (DMP-1), ALP activity, and extracellular matrix mineralization were evaluated. Data were compared using ANOVA (α=5%). Results: Lower cell density was detected only for MTA and CACb+ compared with Control, with areas showing reduced cell spreading. Cell viability was similar among groups at days one and three (p>0.05). CACb+ and MTA showed the lowest cell viability values at day seven (p>0.05). CACb and CACb+ promoted higher ALP and BSP expression compared with CACz (p<0.05); despite that, all cements supported ALP activity. Matrix mineralization were enhanced in CACb+ and MTA. Conclusion: In conclusion, CAC with Bi2O3, but not with ZnO, supported the expression of odontoblastic phenotype, but only the composition with 10% CaCl2 promoted mineralized matrix formation, rendering it suitable for dentin-pulp complex repair

Aging behavior of Y-TZP with bioglass addition and its impact on the flexural strength and osteoblastic cell response

Y-TZP containing Ca2P2O7 are promising bioceramics with potential applications in dental implants and dentistry. These ceramics were developed by the introduction of a refractory sol-gel derived CaO-P2O5-SiO2 bioglass into Y-TZP; Ca2P2O7, and ZrSiO4 phases were formed in situ after sintering. The aging process of Y-TZP with different glass additions was studied. The effect of glass addition on the flexural strength and osteoblastic cell response of non-aged and aged Y-TZP was investigated. Y-TZP exhibited the most pronounced tetragonal (t) to monoclinic (m) transformation of zirconia (ZrO2) during aging; the addition of glass contents between 5 and 20 vol% improved the aging resistant of Y-TZP. Y-TZP flexural strength markedly decreased with increasing aging time; in contrast, the ceramics with glass did not alter their flexural strength upon aging. An increase in the Ca2P2O7 content with increasing glass up to 10 vol%, promoted both the cell viability and the osteogenic differentiation of UMR-106 cells on non-aged and aged samples. The high micro-roughness of Y-TZP with 20 vol% glass after aging, limited the proliferation and the osteogenic potential of the cultures. Y-TZP with 10 vol% glass had the best combination of properties in terms of flexural strength and osteoblast cell response.Fil: Soubelet, Clara Gabriela. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Tecnología de Recursos Minerales y Cerámica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Tecnología de Recursos Minerales y Cerámica; ArgentinaFil: Albano, Maria Patricia. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Tecnología de Recursos Minerales y Cerámica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Tecnología de Recursos Minerales y Cerámica; ArgentinaFil: Zuardi, Leonardo Raphael. Universidade de Sao Paulo; BrasilFil: Secco Martorano, Antonio. Universidade de Ribeirão Preto; BrasilFil: de Castro Raucci, Larissa Moreira Spinola. Universidade de Ribeirão Preto; BrasilFil: Oliveira, PauloTambasco de. Universidade de Sao Paulo; Brasi