Efekat α-tokoferola na količinu slobodnih tiola i SOD aktivnost kod K562 ćelija

We examined effect of α-tocopherol addition and influence of nitric oxide from sodium nitroprusside (SNP) on free thiol content and SOD activity in exponentially growing K562 cells (human erythroleukaemia) in RPMI 1640 (Sigma) supplemented with 2 mM glutamine, 10 % heat-inactivated fetal calf serum and antibiotics at 37 oC in a humidified 5 % carbon dioxide atmosphere. Cells concentration was of 0.85 x 106 per flask. Our results indicate potential dual role of α-tocopherol: induction of more reduced cell state (increased free -SH) and increased resistance of cancer cells to treatment which induce oxidative stress (increased SOD). α-Tocopherol pretreated K562 cells do not expected respond on the SNP treatment. .Ispitivali smo efekat dodatka α-tokoferola i uticaj azot-oksida iz natrijum-nitroprusida (NNP) na sadržaj slobodnih tiol grupa i SOD aktivnost kod K562 ćelija (humana eritroleukemija) u eksponencijalnoj fazi rasta, koje su gajene u RPMI 1640 (Sigma) sa dodatkom 2 mM glutamina, 10 % toplotom-inaktiviranog fetalnog govedjeg seruma i antibiotika, na 37 oC u vlažnoj atmosferi sa 5 % ugljen-dioksida. Koncentracija ćelija bila je 0,85 x 106 po posudi. Naši rezultati ukazuju na moguću dvostruku ulogu α-tokoferola kod ovih ćelija: indukciju više redukovanog stanja (porast slobodnih -SH grupa), ali i porast njihove otpornosti na tretmane koji indukuju oksidacioni stres (porast SOD aktivnosti). α-Tokoferolom pretretirane K562 ćelije ne daju očekivani odgovor na tretman sa NNP.

Efekat α-tokoferola na količinu slobodnih tiola i SOD aktivnost kod K562 ćelija

We examined effect of α-tocopherol addition and influence of nitric oxide from sodium nitroprusside (SNP) on free thiol content and SOD activity in exponentially growing K562 cells (human erythroleukaemia) in RPMI 1640 (Sigma) supplemented with 2 mM glutamine, 10 % heat-inactivated fetal calf serum and antibiotics at 37 oC in a humidified 5 % carbon dioxide atmosphere. Cells concentration was of 0.85 x 106 per flask. Our results indicate potential dual role of α-tocopherol: induction of more reduced cell state (increased free -SH) and increased resistance of cancer cells to treatment which induce oxidative stress (increased SOD). α-Tocopherol pretreated K562 cells do not expected respond on the SNP treatment. .Ispitivali smo efekat dodatka α-tokoferola i uticaj azot-oksida iz natrijum-nitroprusida (NNP) na sadržaj slobodnih tiol grupa i SOD aktivnost kod K562 ćelija (humana eritroleukemija) u eksponencijalnoj fazi rasta, koje su gajene u RPMI 1640 (Sigma) sa dodatkom 2 mM glutamina, 10 % toplotom-inaktiviranog fetalnog govedjeg seruma i antibiotika, na 37 oC u vlažnoj atmosferi sa 5 % ugljen-dioksida. Koncentracija ćelija bila je 0,85 x 106 po posudi. Naši rezultati ukazuju na moguću dvostruku ulogu α-tokoferola kod ovih ćelija: indukciju više redukovanog stanja (porast slobodnih -SH grupa), ali i porast njihove otpornosti na tretmane koji indukuju oksidacioni stres (porast SOD aktivnosti). α-Tokoferolom pretretirane K562 ćelije ne daju očekivani odgovor na tretman sa NNP.

Antioxidative and bifidogenic properties of baker’s yeast β-D-glucan

Ćelijski zid pekarskog kvasca je glavni izvor nesvarljivog polisaharida beta-D-glukana Beta-D-glukan je fiziološki aktivno jedinjenje (opšte poznato kao modulator biološkog odgovora), koji aktivira imuni odgovor domaćina protiv bakterijske, virusne, gljivične i parazitske infekcije, kao i neoplazija. Cilj našeg istraživanja je ispitivanje bifidogenog i antioksidativnog potencijala (1->3),(1->6)-beta-D-glukana izolovanog iz pekarskog kvasca, kao novog prebiotskog dodatka infant formulama. Ukupan broj bifidobakterija nakon 48 h inkubacije u infant formuli sa dodatkom 0,1% (m /V) beta-D-glukana (čistoće 99,54%) bio je značajno viši u odnosu na zrelo majčino mleko, infant formulu sa dodatkom inulina ili infant formulu bez prebiotika, kao referentne supstrate. Promene broja bifidobakterija pra ene su promenama suve biomase, ukupnih bakterijski generisanih kiselina i pH. beta-D-glukan najveće čistoće nema antioksidativnu aktivnost, dok prečišćeni ekstrakti glukana (93,15%, 75,54% i 49,30%) uklanjaju hidroksil radikale. Na osnovu bifidogenog efekta možemo da zaključimo da je beta-D-glukan iz kvasca dobar kandidat kao novi prebiotik za dopunu infant formula.The cell wall of baker’s yeast is a major source of nondigestible polysaccharide beta-glucan ((1->3),(1->6)-beta-D-glucan). Baker’s yeast beta-glucan is a physiologically active compound (generally named “biological response modifier”) and is a broad-spectrum enhancer of host defense against bacterial, viral, fungal and parasitic infections, as well as neoplasia. The aim of our study was to investigate the bifidogenic and antioxidative potential of (1->3),(1->6)-beta-D-glucan isolated from the baker’s yeast (Saccharomyces cerevisiae) in relation to digestibility and purity, as a new infant formula prebiotic supplement. The total number of bifidobacteria after 48 h of incubation in the substrate composed of infant formula supplemented with 0.1 % (m/v) beta-D-glucan (purity 99.54 %) was significantly higher than in mature breast milk, infant formula supplemented with inuline or infant formula without added prebiotic, which were used as reference substrates. Changes in the number of bifidobacteria were followed by the changes in dry biomass, total bacteria-generated organic acids and pH. In contrast, the purest beta-D-glucan did not show any antioxidative activity, while partially purified glucan extracts (93.15%, 75.54% and 49.30%) scavenged hydroxyl radicals. Regarding to digestibility and bifidogenic efficacy Saccharomyces cerevisiae beta-D-glucan could be a candidate as a new infant formula prebiotic supplement.Poster: [https://cer.ihtm.bg.ac.rs/handle/123456789/5059

Antioxidative and bifidogenic properties of baker’s yeast β-D-glucan

Poster presented at XLIX Savetovanje Srpskog hemijskog društva, (13-14. maj 2011., Kragujevac)Abstract: [https://cer.ihtm.bg.ac.rs/handle/123456789/5058

A Comparative Investigation of an in vitro and Clinical Test of the Bifidogenic Effect of an Infant Formula

The bifidogenic effect of an infant formula supplemented with inulin and fructooligosaccharides (4.0 g/l) was examined clinically and in vitro, and compared that of mature breast milk. In a 28-day clinical study, fecal samples of 21 infants, divided into two groups: one receiving the infant formula and the other breast milk, were microbiologically and biochemically examined. In the in vitro investigation, microbiological and biochemical changes in the infant formula and breast milk induced by the action of bifidobacteria isolated from infant feces were examined. There were no significant differences in the fecal numbers of lactobacilli, total aerobes, anaerobes or yeasts and fungi. In contrast, the bifidobacteria numbers in the stools increased significantly during the study in the infants receiving the supplemented formula. The comparative in vitro test showed that the bifidogenic effect was similar for infant formula and breast milk in terms of the number of bifidobacteria. Consumption of infant formula with added inulin and fructooligosaccharides stimulated the bifidogenic effect, both clinically and in vitro. The in vitro test can quickly and objectively determine the bifidogenic effect of infant formula and indicate their quality. However, a clinical test is necessary to determine the acceptance and biological value of infant formula

Infant formula as a substrate for bifidogenesis: An in vitro investigation

Six commercially available infant formulas (IF) without added prebiotic, were investigated as substrates for the growth of bifidobacteria isolated from the feces of 3 day old babies. This investigation tracked, during 48 h, the microbiological and biochemical changes in prepared infant formula meals, caused by the action of bifidobacteria, isolated from babies' feces. Pancreatin digestion preceded inoculation of the bifidobacteria biomass. The reference substrate was mature breast milk. This investigation showed important differences in bifidogenic effect between individual infant formulas and mature breast milk. In relation to dry biomass from the mature breast milk, dry biomass obtained in individual infant formulas ranged from 71.4% (IF-1) to 34.6% (IF-6). The results showed that, with the similar declared nutritive and biological characteristics, there are important differences between individual infant formulas. The results obtained showed that, an in vitro bifidogenic index test which covers tracking of microbiological and biochemical changes should be developed as a usefull tool in determining the suitability of infant formulas and it should be one of the first steps before clinical investigations

Similar bifidogenic effects of the infant formulae with added inuline and breast feeding on gut microflora

The purpose of this study was to determine if there is and what are the differences in the bifidogenic effects and gut microflora of breast-fed and formula-fed newborns (younger than 6 months and children age from 6 to 12 months).Abstracts of the 14th European Congress on Biotechnology (ECB 14)-Symbiosis, Barcelona, Spain, 13-16 September 200

Effect of Prebiotic Infant Formula on The Infant’s Gut Microbial Composition and Anthropometric Factors: Clinical Study

The aim of this study was to investigate the influence of an infant formula containing added inulin and fructooligosaccharides on the gut microflora, as well as on the growth development and development of infants, compared with infants who were exclusively breastfed

Redox status of K562 cells after α-tocopherol treatment

Tocopherols, early described as a vitamin that brings birth, have been identified as redox compounds capable of radical scavenging in a lipid phase. The recent finding that alpha-tocopherol phosphate is present in cells in small amounts assigned alpha-tocopherol to the group of signaling molecules, rather than to that of antioxidants. When antioxidant levels are augmented by either genetic or pharmacologic means the impact on tumor cells both in vitro and in vivo has been to inhibit tumor growth and/or differentiation. We examined effect of alpha-tocopherol addition on free thiol content and SOD activity in K562 cells.Abstracts of the 14th European Congress on Biotechnology (ECB 14)-Symbiosis, Barcelona, Spain, 13-16 Septembe

Investigation of differences in intestinal microbial composition between breast-fed and infant prebiotic formula-fed infants

The aim of this study was investigation of possible differences between the composition of gut microbiota of breast-fed and formula (supplemented with prebiotic)-fed newborns (younger than six months).Abstracts of the 14th European Congress on Biotechnology (ECB 14)-Symbiosis, Barcelona, Spain, 13-16 September 200