Avaliação do estresse oxidativo no sangue de consumidores habituais de suco de laranja

Two biomarkers of oxidative stress were evaluated in healthy volunteers treated with daily doses of orange juice that is well known source of vitamin C and citric flavanones, which have been associated with antioxidant, anti-inflammatory and hypolipidemic effects. The antioxidant capacity in the blood serum was evaluated by studying the scavenging of 2,2'-diphenyl-1-picrylhydrazyl (DPPH) free radical, and also lipid peroxidation was evaluated by the thiobarbituric acid reacting substances (TBARS) assay. The results have shown that the regular consumption of orange juice increased 150% and 200% the serum antioxidant capacity for women and men respectively, but it was no significant change in the serum lipid peroxidation. In conclusion, the increase of flavonones and vitamin C in the body due to the regular intake of orange juice expressively improved the antioxidant capacity, but without significant effect on the lipid peroxidation.Dois biomarcadores do estresse oxidativo foram avaliados em indivíduos saudáveis que receberam doses diárias de suco de laranja, notadamente uma fonte de vitamina C e de flavanonas, que têm sido associadas aos efeitos antioxidante, anti-inflamatório e hipolipidêmico. A capacidade antioxidante do soro sanguíneo foi avaliada através da redução do radical 2,2-difenil-1-picril-hidrazila (DPPH) e a peroxidação lipídica foi avaliada pela presença de substâncias reativas ao ácido tiobarbitúrico (TBARS). Os resultados mostraram que após o período de suplementação com o suco de laranja houve um aumento 150% na capacidade antioxidante no soro das mulheres e 200% no soro dos homens (p<0,001), mas não houve alteração na peroxidação lipídica no sangue dos voluntários. Concluindo, o aumento das reservas de flavanonas e de vitamina C, decorrente da ingestão regular de suco de laranja, melhorou expressivamente a capacidade antioxidante no sangue, sem, entretanto, apresentar efeito sobre a peroxidação lipídica

THE COMFORT OF COMPETENCE AND THE UNCERTAINTY OF ASSESSMENT

C1 - Refereed Journal Articl

Qualea parviflora Mart.: An integrative study to validate the gastroprotective, antidiarrheal, antihemorragic and mutagenic action

Ethnopharmacological relevance: Qualea parviflora Mart. is a medicinal species commonly found in the Brazilian Cerrado biome.Aim of the study: Based on ethnopharmacological data, methanolic extract from Qualea parviflora (QP) bark was evaluated for its antiulcer, analgesic, anti-hemorrhagic, mutagenic and anti-Helicobacter pylori activities.Material and methods: The gastroprotective action of the extract was evaluated in rodent experimental models (HCl/ethanol, ethanol or NSAID). We also evaluated mutagenic effect (Ames assay), anti-Helicobacter pylori, anti-hemorrhagic action, analgesic and inflammatory effects (hot-plate test and carrageenin-induced hind paw edema) of methanolic extract from Qualea parviflora.Results: QP (500 mg/kg, p.o.) was able to protect gastric mucosa against HCl/ethanol solution (77%), absolute ethanol (97%), and also against injurious effect of NSAID (36%). When QP was challenged with sulfhydryl depletor compound, the gastroprotective action of extract was abolished. QP treatment was able to maintain the GSH level and show a concentration-de pendent inhibition effect on the lipid peroxidation. QP present anti-Helicobacter pylori effect (MIC = 75 mu g/mL), anti-hemorrhagic and antidiarrheal action but not present analgesic or anti-inflammatory effect.Conclusion: methanolic extract from Qualea parviflora had gastroprotective effect related to the increase of gastric mucosa defensive factors such PGE(2) levels and maintain the basal gastric glutathione levels. The methanolic extract also showed anti-Helicobacter pylori activity, anti-hemorrhagic effect and antioxidant action, but absence of analgesic, mutagenic and toxic effects, a profile that adds safety to its use. Published by Elsevier B.V.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES