Differentially expressed transcripts mapped to numerous biological processes of the hierarchical GO system

These were combined to the shown eleven superordinate categories. The first number in brackets (preceding the slash) represents the number of transcripts, which significantly map to specific biological processes of the hierarchical GO system. The second number (following the slash) represents all transcripts mapping to the specified biological process without statistical significance.<p><b>Copyright information:</b></p><p>Taken from "Expression profiling of a high-fertility mouse line by microarray analysis and qPCR"</p><p>http://www.biomedcentral.com/1471-2164/9/307</p><p>BMC Genomics 2008;9():307-307.</p><p>Published online 27 Jun 2008</p><p>PMCID:PMC2443385.</p><p></p

Additional file 2: of Selection for female traits of high fertility affects male reproductive performance and alters the testicular transcriptional profile

Distribution of probe cell intensity of unprocessed raw data (.CEL data) (a) Distribution of signal intensity values (.CHP data) after normalization by the Robust Multiarray Average with Signal Space Transformation algorithm (SST-RMA). (b) Consideration of both plots implies an overall successful hybridization experiment for all processed MTA 1.0 microarrays. (ZIP 63 kb

Additional file 1: of Selection for female traits of high fertility affects male reproductive performance and alters the testicular transcriptional profile

List of RT-qPCR primers used. (DOCX 52 kb

Effect of diets during pregnancy and birth.

<p>At day 1 after birth, mothers and litters were examined (group C [n = 50] as the base for C-C and C-HP, group HP [n = 25] is the base for HP-C). (<b>a</b>) Body weight gain of females during pregnancy as difference of weight after delivery vs. day of mating; Litter size (<b>b</b>) and birth weight (<b>c</b>) depending on diet of mother during pregnancy. Data are the means ± SEM, *<i>P</i><0.05, ***<i>P</i><0.001 vs control food (C) using a Student's <i>t</i>-test.</p

Effect of diets during lactation on body weight, survival and milk composition.

<p>Litters were cross-fostered and standardized (8♂, 2♀) at birth. Resulting from dietary treatments 80 males and 20 females counted per group (control-control: C-C, high protein-control: HP-C, and control-high protein C-HP). (<b>a</b>) Pup body weight at weaning (21^st day of life) depending on diet group (C-C: 98 pups; HP-C: 98 pups; C-HP: 83 pups). (<b>b</b>) Number of death in lactation in the three examined groups shown in % (of 100 pups) for period from birth till weaning and (<b>c</b>) Number of death in lactation in the same period subdivided in five-day steps; (<b>d</b>) mRNA levels of whey acidic protein in mammary glands was measured in lactating mice on control diet (C; n = 8) or high-protein diet (HP; n = 9). Data are the means ± SEM; group differences are calculated by ANOVA (<b>3a</b>), Friedman test (<b>3c</b>; <i>P</i> = 0.018 C-HP <i>vs</i> C-C and HP-C) or Student's t-test (<b>3d</b>), *<i>P</i><0.05 <i>vs</i> control food (C), ***<i>P</i><0.001 <i>vs</i> control group (C-C).</p

Survival rate and physiological parameters in adulthood.

<p>(<b>a</b>) Body weight at day 180; (<b>b</b>) Oral glucose tolerance test at day 150 (C-C: n = 10; HP-C: n = 7; C-HP: n = 8); (<b>c</b>) Kaplan Meier curve from weaning until end of experiment (d360); (<b>d</b>) Cardiovascular characterization of mice at day 360 via Tip-catheter (C-C: n = 8; HP-C: n = 7; C-HP: n = 7). Heart rate, left ventricular pressure (LVP), contractility (dP/dtmax), and mean arterial pressure (MAP); Data are the means ± SEM; group differences are calculated by one way of ANOVA (<b>4a, 4d</b>) *<i>P</i><0.05 <i>vs</i> control group (C-C); ^#<i>P</i><0.05 <i>vs</i> high protein-control (HP-C); or two way of ANOVA (<b>4b</b>), **<i>P</i><0.01; group differences (diet effects). For Kaplan-Meier analysis (<b>4c</b>) logrank calculation has been performed; ^$<i>P</i><0.05.</p

Scheme describing the generation of the different diet groups.

<p>Detailed description on the generation of the C-C, C-HP, and HP-C groups has been given in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0017443#s2" target="_blank">Materials and Methods</a> and the Results parts. C  =  control diet, HP  =  high-protein diet.</p

Body weight and fat content at day 360.

<p>At the end of the experiment, the following parameters have been determined (C-C: n = 8; HP-C: n = 8; C-HP: n = 8): (<b>a</b>) Body weight at day 360; (<b>b</b>) Abdominal fat in mice; and (<b>c</b>) Abdominal fat/body weight ratio at day 360. Data are the means ± SEM, *<i>P</i><0.05, <i>vs</i> control group (C-C).</p

Changes in body and organ mass over life-time under chow and HFD.

<p>Effects of a HFD on body weight in male mice (Fig. 2A; until week 27: n>14; week 33 and 39: n>7; week 33: P<0.05). <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079788#pone-0079788-g002" target="_blank">Fig 2B:</a> Effects of a HFD on tissues masses in male mice at an age of 39 weeks (n>7; *: significant effect of line*diet P<0.01 vs. chow diet (100%); °: significant effect of diet as a main effect in both lines: P<0.05 (epidid. fat: epididymal fat mass; Musc. r.f.: Musculus rectus femoris, gastr: gastrocnemius, t.a.: tibialis anterior; sol.: soleus).</p

Compositions of Altromin control chow and high fat diet (HFD; C 1080) used in the present study.

<p>Ether extracts from soybean oil and lard were used for fat isolation. Corn starch, maltodextrin, dextrose and sucrose were used for the preparation of the carbohydrate fraction. Casein was the source for the protein fraction and cellulose was used for crude fiber production.</p