Video recording of freely diffusing 200nm polystyrene nanoparticles using nanoparticle tracking analysis

<p>NTA (Nanosight LM 10) recording of 200nm polystyrene particles (Kisker Biotech PPs-01) which were diluted in essentially particle-free double distilled H2O. The temperature was 24.05 °C. The concentration was amounted to approximately 3x10^8 ml.</p> <p>The video pixel size is 164nm.</p> <p><strong>This video is part of a puplication:</strong></p> <p><strong>Wagner, T., Lipinski, H.-G. & Wiemann, M., 2014. Dark field nanoparticle tracking analysis for size characterization of plasmonic and non-plasmonic particles. Journal of Nanoparticle Research, 16(5), p.2419. Available at: http://link.springer.com/10.1007/s11051-014-2419-x</strong></p

Video recording of freely diffusing 60 nm and 80 nm gold nanoparticles using dark field microscopy

<p>Dark field recordings of 60 nm and 80nm gold particles which were diluted in essentially particle-free double distilled H2O. The temperature was 24 °C. The concentration was amounted to approximately 3x10^9 ml.</p> <p>The video file was captured with a Canon EOS 5D Mark II camera at 25 FPS mounted on a Olympus BX51 microscope (100-fold oil immersion objective) which was illuminated with a CytoViva dark field oil condenser.</p> <p>The video pixel size is 182 nm.</p> <p><strong>This video is part of a puplication:</strong></p> <p><strong>Wagner, T., Lipinski, H.-G. & Wiemann, M., 2014. Dark field nanoparticle tracking analysis for size characterization of plasmonic and non-plasmonic particles. Journal of Nanoparticle Research, 16(5), p.2419. Available at: http://link.springer.com/10.1007/s11051-014-2419-x<br></strong></p

Video recording of freely diffusing 100nm polystyrene nanoparticles using nanoparticle tracking analysis

<p>NTA (Nanosight LM 10) recording of 100nm polystyrene particles (Kisker Biotech PPs-01) which were diluted in essentially particle-free double distilled H2O. The temperature was 22 °C. The concentration was amounted to approximately 3x10^8 ml.</p> <p>The video pixel size is 164nm.</p> <p><strong>This video is part of a puplication: </strong></p> <p><strong>Wagner, T., Lipinski, H.-G. & Wiemann, M., 2014. Dark field nanoparticle tracking analysis for size characterization of plasmonic and non-plasmonic particles. Journal of Nanoparticle Research, 16(5), p.2419. Available at: http://link.springer.com/10.1007/s11051-014-2419-x<br></strong></p

Video recording of freely diffusing 200nm polystyrene nanoparticles using dark field microscopy

<p>Dark field recording of 200nm polystyrene (Kisker Biotech PPs-02) particles which were diluted in essentially particle-free double distilled H2O. The temperature was 25 °C. The concentration was amounted to approximately 3x10^9 ml.</p> <p>The video file was captured with a PCO Edge CCD camera at 30 FPS mounted on a Olympus BX51 microscope (100-fold oil immersion objective) which was illuminated with a CytoViva dark field oil condenser. The pixel size of the video is 63nm.</p> <p>Please note that the video is splitted into mutiple archives. Please download all archives to get the full video.</p> <p><strong>This video is part of a  puplication:</strong></p> <p><strong>Wagner, T., Lipinski, H.-G. & Wiemann, M., 2014. Dark field nanoparticle tracking analysis for size characterization of plasmonic and non-plasmonic particles. Journal of Nanoparticle Research, 16(5), p.2419. Available at: http://link.springer.com/10.1007/s11051-014-2419-x</strong></p

Video recording of freely diffusing 100nm polystyrene nanoparticles using dark field microscopy

<p>Dark field recordings of 100nm polystyrene (Kisker Biotech PPs-01) particles which were diluted in essentially particle-free double distilled H2O. The temperature was 24 °C. The concentration was amounted to approximately 3x10^9 ml.</p> <p>The video file was captured with a PCO Edge CCD camera at 30 FPS mounted on a Olympus BX51 microscope (100-fold oil immersion objective) which was illuminated with a CytoViva dark field oil condenser. The video pixel size is 63nm.</p> <p>Please note that the video is splitted into mutiple archives. Please download all archives to get the full video.</p> <p><strong>This video is part of a puplication:</strong></p> <p><strong>Wagner, T., Lipinski, H.-G. & Wiemann, M., 2014. Dark field nanoparticle tracking analysis for size characterization of plasmonic and non-plasmonic particles. Journal of Nanoparticle Research, 16(5), p.2419. Available at: http://link.springer.com/10.1007/s11051-014-2419-x</strong></p> <p> </p

NanoTrackJ: Size characterization of freely diffusing nanoparticles by nanoparticle tracking

<p>NanoTrackJ is an ImageJ Plugin to characterize the size of nanoparticles in a liquid suspension. It analyzes videos of diffraction patterns of nanoparticles and track the change in position of each diffraction pattern to estimate the size via stokes-einstein.</p> <p>It is developed and maintained by Thorsten Wagner ([email protected]) of the Biomedical Imaing Group at the University of Applied Sciences Dortmund.</p> <p><strong>This site is part of a puplication:</strong></p> <p><strong>Wagner, T., Lipinski, H.-G. & Wiemann, M., 2014. Dark field nanoparticle tracking analysis for size characterization of plasmonic and non-plasmonic particles. Journal of Nanoparticle Research, 16(5), p.2419. Available at: http://link.springer.com/10.1007/s11051-014-2419-x</strong></p> <p><strong><br></strong></p> <p><strong>How to install</strong></p> <p> 1. Download the NanoTrackJ.zip</p> <p> 2. Unzip the file</p> <p> 3. Copy the NanoTrackJ_.jar file to th ImageJ/Plugins folder</p> <p> 4. Copy all files in jars folder to the Image/Plugins/jars folder</p> <p>5. Restart ImageJ</p> <p><strong><br>Newer Versions and the documentation of NanoTrackJ can found on:</strong></p> <p><strong>https://sourceforge.net/projects/nanotrackj/</strong></p> <p> </p> <p> </p

Mean squared displacment curve for simulated motion models.

<p>Mean squared displacment curves based on simulated trajectories for directed motion (black), normal diffusion (blue), anomalous diffusion (green) and confined diffusion (red).</p

Motion class proportions of classified positions of experimental trajectories captured by NanoSight NTA (N = 334), darkfield microscopy (N = 90), confocal laser scanning microscopy (N = 41) and fluorescence microscopy (N = 28).

<p>Motion class proportions of classified positions of experimental trajectories captured by NanoSight NTA (N = 334), darkfield microscopy (N = 90), confocal laser scanning microscopy (N = 41) and fluorescence microscopy (N = 28).</p

Histogram distances for different resampling rates.

<p>The lowest distance for each method is highlighted in bold.</p

NP trajectories in live V79 fibroblasts as classified by the program.

<p>Several cells are gathered within the field of view (left image). While cell borders are not visible in this focal plane, nuclear envelopes stand out clearly under darkfield illumination (white arrows). A total of 246 particle trajectories was identified as either normal diffusion (red), confined diffusion (yellow), anomalous diffusion (green) or directed motion (magenta). Boxed areas (A-D) show selected cases of directed motion. The time durations of the directed motion were 14.6s (A), 7.4s (B), 10s (C), 6.4s (D1), 10.8s (D2). Note that directed motion ends up with confined or anomalous diffusion (A, B, D) or emanates from a phase of free diffusion (C). Bar: 5 μm.</p