Relative abundance of CD3^pos cells, CD8^pos -, CD4^pos -T cells and Tregs in the intestinal mucosa.

<p>A) T cells are more or less evenly distributed in the gut B) CD8^pos T cells are enriched in the human gastric corpus and antrum. C) CD3^posCD4^pos T cells are predominantly found in the lower intestinal tract. D) The relative abundance of CD4^posCD25^highCD127^low/negFOXP3 cells was highest in the appendiceal orifice region and the ascending colon. The figures represent cumulative flow cytometry data from all study participants (N=16) from all seven biopsy regions including the gastric corpus (GC), gastric antrum (GA), duodenum (DD), terminal ileum (TI), appendiceal orifice (AO), ascending colon (AC) and sigmoid colon (SC). Unless otherwise indicated, differences were not significant. *P<0.05; **P<0.01; ***P<0.001.</p

Representative gating strategy for the FACS analysis of lamina propria CD3+, CD4+, CD8+ and regulatory T-cells from pinch biopsies obtained from the appendiceal orifice region of one healthy subject.

<p>Representative gating strategy for the FACS analysis of lamina propria CD3+, CD4+, CD8+ and regulatory T-cells from pinch biopsies obtained from the appendiceal orifice region of one healthy subject.</p