Colonization of diatoms and bacteria on artificial substrates in the northeastern coastal Adriatic Sea

Every surface that is immersed in seawater becomes rapidly covered with an unavoidable biofilm. Such biofilm formation, also known as fouling, is a complex multistage process and not yet thoroughly investigated. In this study, the succession of diatoms and bacteria was investigated during a one month exposure on an artificial substrate of plexiglass (polymer of methyl methacrylate) mounted above the seafloor at a depth of 5 m. For biofilm analyses, the fouling was investigated using selective agar plates, epifluorescence, light and electronic microscopy, as well as high performance liquid chromatography (HPLC) pigment analysis. During biofilm development, the abundance of all biofilm components increased and reached maximum values after a one month exposure. In the bacterial community, heterotrophic marine bacteria were dominant and reached 1.96 ± 0.79 × 104 colony forming units (CFU) cm–2. Despite the fact that faecal coliforms and intestinal enterococci were detected in the water column, faecal coliforms were not detected in the biofi lm and intestinal enterococci appeared after one month of exposure but in the negligible number of 60 ± 10 CFU cm–2. The phototrophic component of the biofilm was dominated by diatoms and reached a concentration of 6.10 × 105 cells cm–2, which was supported by pigment analysis with fucoxanthin as dominant pigment in a concentration up to 110 ng cm–2. The diatom community was dominated by Cylindrotheca closterium and other pennate benthic diatoms. A detailed taxonomic analysis by electronic microscopy revealed 30 different taxa of diatoms. The study confirmed that a plexiglass surface in a marine environment is susceptible to biofouling within 30 days of contact. Furthermore, the colonization process sequence firstly involved bacteria and cyanobacteria, and secondly diatoms, which together formed a primary biofilm in the sea

Development of periphytic diatoms on different artificial substrates in the Eastern Adriatic Sea

The settling of diatoms as fouling organisms on a certain substrate is greatly influenced by substrate characteristics and the preferences of a diatom community and diatom species. A distinction among substrates can be made by analysing the specific abundance and composition of diatoms on different substrates. In this study, 11 different artificial substrates were exposed to a marine environment for a period of 30 days. Abundance and taxonomic composition of periphytic diatoms was determined on each of the substrates and on shoots of the marine seagrass Posidonia oceanica. The aim was to compare diatom community structure on different newly colonized surfaces. On all surfaces examined, periphytic diatoms were the pioneering organisms with differences in quantitative and qualitative composition on the different substrates. Taxonomic analysis of diatom communities on the substrates examined revealed 41 diatom taxa, with the dominant genera Cylindrotheca, Amphora, Nitzschia, Cocconeis and Navicula. Given that all the examined artificial substrates were solid materials, differences in the abundance and species composition of diatoms found between the materials point to the substrates’ physical and chemical characteristics as a major influence on the final settling of diatoms. Knowledge from investigating the settlement of fouling organisms on anthropogenic substrates can have future use in management of waste materials that end up in the marine environment

Assessment of bacterial endosymbiont diversity in Otiorhynchus spp. (Coleoptera: Curculionidae) larvae using a multitag 454 pyrosequencing approach

<p>Abstract</p> <p>Background</p> <p>Weevils of the genus <it>Otiorhynchus</it> are regarded as devastating pests in a wide variety of horticultural crops worldwide. So far, little is known on the presence of endosymbionts in <it>Otiorhynchus</it> spp.. Investigation of endosymbiosis in this genus may help to understand the evolution of different reproductive strategies in these weevils (parthenogenesis or sexual reproduction), host-symbiont interactions, and may provide a future basis for novel pest management strategy development. Here, we used a multitag 454 pyrosequencing approach to assess the bacterial endosymbiont diversity in larvae of four economically important <it>Otiorhynchus</it> species.</p> <p>Results</p> <p>High-throughput tag-encoded FLX amplicon pyrosequencing of a bacterial <it>16S</it> rDNA fragment was used to characterise bacterial communities associated with different <it>Otiorhynchus</it> spp. larvae. By sequencing a total of ~48,000 PCR amplicons, we identified 49 different operational taxonomic units (OTUs) as bacterial endosymbionts in the four studied <it>Otiorhynchus</it> species. More than 90% of all sequence reads belonged either to the genus <it>Rickettsia</it> or showed homology to the phylogenetic group of “<it>Candidatus</it> Blochmannia” and to endosymbionts of the lice <it>Pedicinus obtusus</it> and <it>P. badii</it>. By using specific primers for the genera <it>Rickettsia</it> and “<it>Candidatus</it> Blochmannia”, we identified a new phylogenetic clade of <it>Rickettsia</it> as well as “<it>Candidatus</it> Nardonella” endosymbionts in <it>Otiorhynchus</it> spp. which are closely related to “<it>Candidatus</it> Blochmannia” bacteria.</p> <p>Conclusions</p> <p>Here, we used multitag 454 pyrosequencing for assessment of insect endosymbiotic communities in weevils. As 454 pyrosequencing generates only quite short sequences, results of such studies can be regarded as a first step towards identifying respective endosymbiotic species in insects. In the second step of our study, we analysed sequences of specific gene regions for a more detailed phylogeny of selected endosymbiont genera. As a result we identified the presence of <it>Rickettsia</it> and “<it>Candidatus</it> Nardonella<it>”</it> endosymbionts in <it>Otiorhynchus</it> spp.. This knowledge is an important step in exploring bacteria-insect associations for potential use in insect pest control.</p

The freshwater jellyfish Craspedacusta sowerbii Lankester, 1880 (Limnomedusa: Olindiidae) in Germany, with a brief note on its nomenclature

The freshwater jellyfish Craspedacusta sowerbii Lankester is distributed worldwide in temperate, freshwater habitats. However, such a successful, worldwide dispersion of the medusa and its minute, less obvious polyp is unique among the freshwater jellyfish species (Limnomedusae, Olinidiae). Although numerous reports were given shortly after its discovery, the interest did not cease over the years. In cooperation with the German Underwater Federation (Verband Deutscher Sporttaucher e. V.) additional data of freshwater jellyfish occurrences could be obtained. In combination with previously published discovery reports, these data provide an area- wide observation of the distribution of freshwater jellyfish within the Federal Republic of Germany, adding 21 to the previously known C. sowerbii locations. Indications are that there is a far more wide spread distribution of the medusa than thought and the potentially even wider distribution of the often times overlooked polyp

A metabarcode based (species) inventory of the northern Adriatic phytoplankton

The northern Adriatic is characterised as the coldest and most productive marine area of the Mediterranean, which is due to high nutrient levels introduced by river discharges, the largest of which is the Italian Po River (at the same time also the largest freshwater input into the Mediterranean). The northern Adriatic is a very shallow marine ecosystem with ocean current patterns that result in long retention times of plankton in the area. The northern Adriatic phytoplankton biodiversity and abundance are well-studied, through many scientific and long-term monitoring reports. These datasets were based on phytoplankton morphological traits traditionally obtained with light microscopy. The most recent comprehensive eastern Adriatic phytoplankton checklist was published more than 20 years ago and is still valuable today. Since phytoplankton taxonomy and systematics are constantly being reviewed (partly also due to new molecular methods of species identification that complement classical methodologies), checklists need to be updated and complemented. Today, metabarcoding of molecular markers gains more and more importance in biodiversity research and monitoring. Here, we report the use of high throughput sequencing methods to re-examine taxonomic richness and provide updated knowledge of phytoplankton diversity in the eastern northern Adriatic to complement the standardised light microscopy method.This study aimed to report an up-to-date list of the phytoplankton taxonomic richness and phylogenetic relationships in the eastern northern Adriatic, based on sequence variability of barcoding genes resolved with advanced molecular tools, namely metabarcoding. Here, metabarcoding is used to complement standardised light microscopy to advance conventional monitoring and research of phytoplankton communities for the purpose of assessing biodiversity and the status of the marine environments. Monthly two-year net sampling targeted six phytoplankton groups including Bacillariophyceae (diatoms) and Chrysophyceae (golden algae) belonging to Ochrophyta, Dinophyceae (dinoflagellates), Cryptophyceae (cryptophytes), Haptophyta (mostly coccolithophorids) and Chlorophyta with Prasinophyceae (prasinophytes) and Chlorophyceae (protist green algae). Generated sequence data were taxonomically assigned and redistributed in two kingdoms, five classes, 32 orders, 49 families and 67 genera. The most diverse group were dinoflagellates, comprising of 34 found genera (48.3%), following by diatoms with 23 (35.4%) and coccolithophorids with three genera (4.0%). In terms of genetic diversity, results were a bit different: a great majority of sequences with one nucleotide tolerance (ASVs, Amplicon sequence variants) assigned to species or genus level were dinoflagellates (83.8%), 13.7% diatoms and 1.6% Chlorophyta, respectively. Although many taxa have not been detected that have been considered as common in this area, metabarcoding revealed five diatoms and 20 dinoflagellate genera that were not reported in previous checklists, along with a few species from other targeted groups that have been reported previously. We here describe the first comprehensive 18S metabarcode inventory for the northern Adriatic Sea