Course of <i>Encepahlitozoon cuniculi</i> genotype II infection, including pattern of spore shedding and dissemination of infection to selected organs and tissues.

<p><b>a)</b> SCID mice, <b>b)</b> SCID mice treated with albendazole, <b>c)</b> BALB/c mice and BALB/c mice re-infected in chronic stage of infection, <b>d)</b> BALB/c mice treated with albendazole and BALB/c mice treated with albendazole with following re-infection, <b>e)</b> BALB/c mice immunosuppressed in chronic stage of infection, <b>f)</b> BALB/c mice immunosuppressed after albendazole treatment. <b>Light-gray field</b> – albendazole treatment; <b>dark-gray field</b> – dexamethasone immunosuppression; <b>black line</b> – course of <i>E. cuniculi</i> infection; <b>black dash line</b> - course of <i>E. cuniculi</i> re-infection; <b>cross</b> – <i>E. cuniculi</i> positive organ during primarily infection; <b>ring</b> – <i>E. cuniculi</i> positive organ during re-infection; <b>black square</b> – spores shedding during primarily infection; <b>black circle</b> – spores shedding during re-infection.</p

Design of experiments.

<p><b>^a</b>inoculation with 200 ul sterilized deionised water; <b>^b</b>infection with 10⁷<i>E. cuniculi</i> spores in 0.2 ml of sterilized deionised water; INF – infection; REINF – reinfection (black column); IMSUP – dexamethasone immunosuppression (highlighted in dark grey); TREAT – albendazole treatment (highlighted in light grey); <b>n1–</b> number of used animals; <b>n2</b>– number of dissected animals; <b>NS</b> – not shown; <b>x</b> – not observed due to mouse death; <b>DPI</b> – day post infection;</p

<i>Enterocytozoon bieneusi</i>, <i>Encephalitozoon cuniculi</i>, <i>Cryptosporidium</i> spp. and <i>Giardia intestinalis</i> infection in wild western lowland gorillas (<i>Gorilla gorilla gorilla</i>) under different levels of human contact.

<p><b>D</b> =  <i>E. bieneusi</i> genotype D; <b>gorilla 1</b>  =  <i>E. bieneusi</i> genotype gorilla 1; <b>gorilla 2</b>  =  <i>E. bieneusi</i> genotype gorilla 2; <b>gorilla 3</b>  =  <i>E. bieneusi</i> genotype gorilla 3; <b>EC I</b>  =  <i>E. cuniculi</i> genotype I; <b>EC II</b>  =  <i>E. cuniculi</i> genotype II; <b>A</b>  =  <i>Giardia intestinalis</i> assemblage A; <b>n¹</b> number of samples; <b>n²</b> number of animals sampled.</p

Neighbour-joining tree based on nucleotide sequences of the whole ITS region of Enterocytozoon bieneusi isolates, including our new sequences (underlined).

<p>Genotypes previously found in apes and humans are shaded. The host is listed for each sample. Values on branches are percent bootstrapping using 1 000 replicates. The bootstrap proportions greater than 50% are shown on each branch. Nucleotide sequences generated from this study are underlined and are deposited in the GenBank under Accession Nos. JQ837793-JQ837800.</p

Enterocytozoon bieneusi, Encephalitozoon cuniculi, Cryptosporidium spp. and Giardia intestinalis infection in humans, wild and domestic animals.

<p><b>n</b>  =  number of samples; <b>EC I</b>  =  <i>E. cuniculi</i> genotype I; <b>EC II</b>  =  <i>E. cuniculi</i> genotype II; <b>E</b>  =  <i>Giardia intestinalis</i> assemblage E; <b>A</b>  =  <i>Giardia intestinalis</i> assemblage A.</p