Developmental expression and differentiation-related neuron-specific splicing of (Mtss1) in normal and transformed cerebellar cells-6

<p><b>Copyright information:</b></p><p>Taken from "Developmental expression and differentiation-related neuron-specific splicing of (Mtss1) in normal and transformed cerebellar cells"</p><p>http://www.biomedcentral.com/1471-213X/7/111</p><p>BMC Developmental Biology 2007;7():111-111.</p><p>Published online 9 Oct 2007</p><p>PMCID:PMC2194783.</p><p></p>deep cerebellar mass is relatively devoid of signal, as is the external granule cell layer. The latter can be readily recognized as a dense band at the surface in panel B, which shows counterstaining with Hoechst 33342. : At p3 (C), p5 (D) and p8 (E, F), staining can be unambiguously attributed to cells in the inner part of the external granule cell layer, granule cells in the inner granule cell layer, and Purkinje neurons, which show an increasingly strong signal in the perikaryon. Note that the outer part of the EGL and the (prospective) white matter are devoid of signal. : Between postnatal day 15 (G, H) and 21 (I), granule cells in the (internal) granule cell layer cease to express Mtss1. : Adult. Staining is limited to Purkinje cell perikarya. All sections were obtained from the central vermis, except the one shown in panel I, which originates from the lateral vermis, and are cut in the sagittal plane. Anterior is to the left. Bar (in A, J) = 125 μm for panels D, E and insert in C; 250 μm for panels A, B, C, F, H and I; 500 μm for panels G and K; and 1 mm for panel J

Developmental expression and differentiation-related neuron-specific splicing of (Mtss1) in normal and transformed cerebellar cells-5

<p><b>Copyright information:</b></p><p>Taken from "Developmental expression and differentiation-related neuron-specific splicing of (Mtss1) in normal and transformed cerebellar cells"</p><p>http://www.biomedcentral.com/1471-213X/7/111</p><p>BMC Developmental Biology 2007;7():111-111.</p><p>Published online 9 Oct 2007</p><p>PMCID:PMC2194783.</p><p></p>ructures were compared using the Vector Alignment Search Tool (VAST), and visualized with Cn3D 4.1. Alignment is color-coded (red, high), except for the region containing the nuclear localization signal, which is marked yellow to facilitate orientation. : The putative nuclear localization signals (NLS; red, basic amino acids) in the IMD are marked on the chain of the IMD domain pointing to the left, whereas the putative export signal is marked white on the chain pointing to the right. Potential target sites for phosphokinases within/close to the NLS are marked green, whereas the remaining amino acids in the NLS are marked blue (see Fig 5B for sequence details). Panel B was generated using the SwissPDB viewer

Schematic view of the organization of the murine Mtss1 gene based on Ensemble entry ENSMUSG00000022353

<p><b>Copyright information:</b></p><p>Taken from "Developmental expression and differentiation-related neuron-specific splicing of (Mtss1) in normal and transformed cerebellar cells"</p><p>http://www.biomedcentral.com/1471-213X/7/111</p><p>BMC Developmental Biology 2007;7():111-111.</p><p>Published online 9 Oct 2007</p><p>PMCID:PMC2194783.</p><p></p> The 5' and 3' UTRs (dark boxes) are not drawn to scale, nor are the intronic regions. Comparison of the murine gene with that of the rat (ENSRNOG00000009001; transcript ENSRNOT00000023505) further suggests that the region labeled here as exon 15 may contain an additional, 108 bp long intron (I, marked in light gray) which would result in the division of exon 15 into two exons of 354 and 239 bps, respectively. Also, the length of exon 14 may either encompass 163 (ENSMUST00000080371) or 208 bp (ENSMUST00000036782). The 45 bps in question are located C-terminal and alternatively form part of the intron separating exons 14 and 15. Regions covered by the in situ hybridization probes used are labeled by horizontal lines A and B. Arrows mark positions of primers used (black arrows, forward primers; open arrows, reverse primers). Schematic view of the derived protein. The N-terminal IMD domain and the C-terminal WH2 domain are shown as gray boxes. The localization of the putative nuclear import (I) and export (E) motives are indicated as black and white boxes, respectively. Expression of Mtss1 splice variants in the early postnatal and adult murine cerebellum. Use of primers located in exons 7 and 13 (primers 2, 5; panel C) reveals the existence of 4 splice variants (the band representing exon combination 11/12/12a/13 reproduces only very weakly here) in the developing and adult cerebellum, as does the use of primers located in exons 11 and 13 (primers 4, 5; panel D). Note that the relative intensity in particular of the band representing splice variants comprising exons 11/12/13 and 11/12a/13 varies during development. The band labeled gapdh is a loading control. Numbers indicate days postnatal; Ad, adult. The arrow indicates a spurious amplificate from an unrelated sequence: This was verified by sequencing, as were the products labeled as bands 11/12/12a/13, 11/12/13, 11/12a/13 and 11/13

Developmental expression and differentiation-related neuron-specific splicing of (Mtss1) in normal and transformed cerebellar cells-3

<p><b>Copyright information:</b></p><p>Taken from "Developmental expression and differentiation-related neuron-specific splicing of (Mtss1) in normal and transformed cerebellar cells"</p><p>http://www.biomedcentral.com/1471-213X/7/111</p><p>BMC Developmental Biology 2007;7():111-111.</p><p>Published online 9 Oct 2007</p><p>PMCID:PMC2194783.</p><p></p>oblastoma samples D86, D978, D82, D1401, and D1062; lanes H1, H2: fetal human cerebellar samples R1626 and R1628. Lane C is a negative control. The band indicative of the splice variant containing exons 11/12a/12/13 is hardly visible in this reproduction. : In DAOY (D) and D-283Med medulloblastoma cell lines, bands representing the splice variants 11/12/13 and 11/13 predominate. Note absence of the band indicative of the splice variant 11/12a/13, which should comigrate with the prominent band of sample 10 shown for comparison. Lane C is a negative control