1 research outputs found
Liposomes as Drug Deposits in Multilayered Polymer Films
The ex vivo growth of implantable
hepatic or cardiac tissue remains a challenge and novel approaches
are highly sought after. We report an approach to use liposomes embedded
within multilayered films as drug deposits to deliver active cargo
to adherent cells. We verify and characterize the assembly of poly(l-lysine) (PLL)/alginate, PLL/poly(l-glutamic acid),
PLL/poly(methacrylic acid) (PMA), and PLL/cholesterol-modified PMA
(PMA<sub>c</sub>) films, and assess the myoblast and hepatocyte adhesion
to these coatings using different numbers of polyelectrolyte layers.
The assembly of liposome-containing multilayered coatings is monitored
by QCM-D, and the films are visualized using microscopy. The myoblast
and hepatocyte adhesion to these films using PLL/PMA<sub>c</sub> or
poly(styrenesulfonate) (PSS)/poly(allyl amine hydrochloride) (PAH)
as capping layers is evaluated. Finally, the uptake of fluorescent
lipids from the surface by these cells is demonstrated and compared.
The activity of this liposome-containing coating is confirmed for
both cell lines by trapping the small cytotoxic compound thiocoraline
within the liposomes. It is shown that the biological response depends
on the number of capping layers, and is different for the two cell
lines when the compound is delivered from the surface, while it is
similar when administered from solution. Taken together, we demonstrate
the potential of liposomes as drug deposits in multilayered films
for surface-mediated drug delivery