Experimental set-up of the 50 Hz MF exposure of the present study.

<p>Details are provided in the main text.</p

Representative autoradiographs of Feulgen-prestained and Light Green SF Yellowish post stained paraffin sections analyzed in the present study.

<p>The photomicrographs show details of the mouse liver after an 8-week 50 Hz MF exposure with 1.0 mT with (A, B) or without (C) injection of ³H-TdR 5 min after the end of the MF exposure. Both autoradiographs were exposed in the same box for 125 days, i.e. under completely identical conditions. In A, a few (out of many) individual silver grains found over the nucleus (arrows) and cytoplasm (arrowheads) of two hepatocytes are marked; the cell bounds are indicated. In B, the arrow points to a cell that was in S phase after injection of ³H-TdR (i.e., during the last two hours of life). In C, single silver grains found over the nucleus (arrow) or cytoplasm (arrowhead) of hepatocytes are marked, representing autoradiographic background. In all panels A to C, black dots and asterisks indicate small and larger sections of liver sinusoids, respectively. The scale bar represents 25 µm.</p

Results of the autoradiographic analyses.

<p>The graphs show mean and standard error of the mean (SEM) of grain numbers representing UDS (<b>A</b>, <b>D</b>, <b>G</b>, <b>J</b>), unrepaired nDNA SSB/ISNT (<b>B</b>, <b>E</b>, <b>H</b>, <b>K</b>) and mtDNA synthesis (<b>C</b>, <b>F</b>, <b>I</b>, <b>L</b>) of neurons in the caudate nucleus in the brain (<b>A–C</b>), epithelial cells in the choroid plexus of the fourth ventricle in the brain (<b>D–F</b>), epithelial cells of the cortical collecting duct in the kidney (<b>G–I</b>), and pericentral hepatocytes in the liver (<b>J–L</b>) after sham-exposure (open bars), MF exposure with 0.1 mT for eight weeks (gray bars), or MF exposure with 1.0 mT for eight weeks (closed bars). For a detailed description of the generation of these grain numbers and grain densities see Section “Evaluation of Autoradiographs” in the main text. Statistically significant differences between groups are indicated.</p