Development of plant height from the soil surface to the top ligule.

<p>A: AS experiment, the final height is achieved at week 22. B: SS experiment, the final height is achieved at week 16. C: plants at the 7^th week in AS experiment, the yellow line shows the site of the top ligule in each genotype and the two parents.</p

Effects of <i>Rht12</i> on coleoptile length and root traits of different groups of the F_2:3 lines at the seedling stage.

*<p>Mean value of the longest root length in each set of lines. Data are means ±SD of each genotype. Data of the two parents were not considered in the statistical significance testing. Different letters within columns indicate statistically significant differences (<i>P</i><0.05).</p

(A) Western analysis showing immunodetection of AtGCN2 in wild-type Arabidopsis, ecotype Landsberg , and the absence of AtGCN2 from mutant line GT8359

(B) Diagram showing the insertion site and orientation of the element in the first intron of the gene in Genetrap mutant line GT8359 (see for full information on intron positions).<p><b>Copyright information:</b></p><p>Taken from "GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2α in Arabidopsis"</p><p></p><p>Journal of Experimental Botany 2008;59(11):3131-3141.</p><p>Published online 4 Jul 2008</p><p>PMCID:PMC2504353.</p><p></p

Seminal root morphology of the dwarf and tall lines at the seedling stage determined by the Cigar method.

<p>Panel A shows the dwarf lines and B shows the tall lines. The tall lines always have two more seminal roots than the dwarf lines (<i>P</i><0.05).</p

Culm morphology of the four genotypes in the AS and SS experiments.

<p>A: the mature plant morphology of the four genotypes and the two parents in the AS experiment. B: schematic representation of internode elongation patterns of the four genotypes and the two parents in AS experiment. C: Schematic representation of internode elongation patterns of the four genotypes in the SS experiment.</p

(A) Alignment of amino acids surrounding the phosphorylation site at the N-terminal end of eIF2α showing conservation of the sequence in plants, animals and fungi

All sequences come from the GENBANK database with the exception of that of wheat which was reported by Browning (). The target serine is highlighted. (B) Western analyses showing immunodetection of AtGCN2, phosphorylated AteIF2α (AteIF2α-) or total AteIF2α in crude protein extracts from seedlings of Arabidopsis, ecotype Landsberg or gene trap mutant line GT8359, as indicated. The seedlings were treated with water, a herbicide or a herbicide plus the appropriate amino acids to mitigate the effect of the herbicide, as indicated. Note that the antisera reacted with other proteins as well; a strip of the western showing the reaction with a protein of the expected size is shown in each case.<p><b>Copyright information:</b></p><p>Taken from "GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2α in Arabidopsis"</p><p></p><p>Journal of Experimental Botany 2008;59(11):3131-3141.</p><p>Published online 4 Jul 2008</p><p>PMCID:PMC2504353.</p><p></p

(A) Wild-type Arabidopsis, ecotype Landsberg , and gene trap mutant line GT8359 growing in soil

(B) Arabidopsis seedlings growing on agar plates. The plate on the left is a control while the other plates were treated with herbicide as indicated. In each case wild-type Arabidopsis, ecotype Landsberg was sown on the top half of the plate while Genetrap mutant line GT8359 was sown on the bottom half, as indicated. (C) As for (B), except that the seedlings were fed with the appropriate amino acids to compensate for the herbicide treatments; these were phenylalanine, tryptophan and tyrosine in the case of Glyphosate, histidine in the case of IRL 1803 and isoleucine, leucine and valine for Chlorsulfuron.<p><b>Copyright information:</b></p><p>Taken from "GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2α in Arabidopsis"</p><p></p><p>Journal of Experimental Botany 2008;59(11):3131-3141.</p><p>Published online 4 Jul 2008</p><p>PMCID:PMC2504353.</p><p></p

Seedling vigour of different groups of the F_2:3 lines in the autumn-sown (AS) and spring-sown (SS) experiments.

<p>All data are means ±SD of each genotype. Data of the two parents were not considered in the statistical significance testing. Different letters within columns indicate statistically significant differences (<i>P</i><0.05).</p

Effects of <i>Rht12</i> on yield components and harvest index of different groups of the F_2:3 lines in the autumn-sown (AS) and spring-sown (SS) experiments.

*<p>These data are the mean values of 25∼40 plants. All data are means ±SD of each genotype. Data of the two parents were not considered in the statistical significance testing. Different letters within columns indicate statistically significant differences (<i>P</i><0.05).</p

Effects of <i>Rht12</i> on the spike characters and fertility of different groups of the F_2:3 lines in the autumn-sown (AS) and spring-sown (SS) experiments.

*<p>Fertility is estimated as the ratio of fertility florets to total floret per spike of the main shoot spike. All data are means ±SD of each genotype. Data of the two parents were not considered in the statistical significance testing. Different letters within columns indicate statistically significant differences (<i>P</i><0.05).</p