Fast recognition of alternating groups of unknown degree

We present a constructive recognition algorithm to decide whether a given black-box group is isomorphic to an alternating or a symmetric group without prior knowledge of the degree. This eliminates the major gap in known algorithms, as they require the degree as additional input. Our methods are probabilistic and rely on results about proportions of elements with certain properties in alternating and symmetric groups. These results are of independent interest; for instance, we establish a lower bound for the proportion of involutions with small support.Comment: 31 pages, submitted to Journal of Algebr

A comparison between Pa alpha and H alpha emission: The relation between HII region mean reddening, local gas density and metallicity

We measure reddenings to HII regions in NGC 2903, NGC 1512, M51, NGC 4449 and NGC 6946 from Hubble Space Telescope Pa alpha and H alpha images. Extinctions range from A_V ~ 5 - 0 depending upon the galaxy. For the galaxies with HST images in both lines, NGC 2903, NGC 1512 and M51, the Pa alpha and H alpha emission are almost identical in morphology which implies that little emission from bright HII regions is hidden from view by regions of comparatively high extinction. The scatter in the measured extinctions is only +- 0.5 mag. We compare the reddenings we measure in five galaxies using the Pa alpha to H alpha ratios to those measured previously from the Balmer decrement in the LMC and as a function of radius in M101 and M51. We find that luminosity weighted mean extinctions of these ensembles of HI regions are correlated with gas surface density and metallicity. The correlation is consistent with the mean extinction depending on dust density where the dust to gas mass ratio scales with the metallicity. This trend is expected if HII regions tend to be located near the mid-plane of a gas disk and emerge from their parent molecular clouds soon after birth. In environments with gas densities below a few hundred Msol/pc^2 star formation rates estimated from integrated line fluxes and mean extinctions are likely to be fairly accurate.Comment: accepted for publication in A

Constitutive Expressor of Pathogenesis-Related Genes5 affects cell wall biogenesis and trichome development

<p>Abstract</p> <p>Background</p> <p>The Arabidopsis thaliana <it>CONSTITUTIVE EXPRESSOR OF PATHOGENESIS-RELATED GENES5 </it>(<it>CPR5</it>) gene has been previously implicated in disease resistance, cell proliferation, cell death, and sugar sensing, and encodes a putative membrane protein of unknown biochemical function. Trichome development is also affected in <it>cpr5 </it>plants, which have leaf trichomes that are reduced in size and branch number.</p> <p>Results</p> <p>In the work presented here, the role of <it>CPR5 </it>in trichome development was examined. Trichomes on <it>cpr5 </it>mutants had reduced birefringence, suggesting a difference in cell wall structure between <it>cpr5 </it>and wild-type trichomes. Consistent with this, leaf cell walls of <it>cpr5 </it>plants contained significantly less paracrystalline cellulose and had an altered wall carbohydrate composition. We also found that the effects of <it>cpr5 </it>on trichome size and endoreplication of trichome nuclear DNA were epistatic to the effects of mutations in <it>triptychon </it>(<it>try</it>) or overexpression of <it>GLABRA3</it>, indicating that these trichome developmental regulators are dependant on <it>CPR5 </it>function for their effects on trichome expansion and endoreplication.</p> <p>Conclusion</p> <p>Our results suggest that <it>CPR5 </it>is unlikely to be a specific regulator of pathogen response pathways or senescence, but rather functions either in cell wall biogenesis or in multiple cell signaling or transcription response pathways.</p

The Mass-Metallicity Relation at z~2

We use a sample of 87 rest-frame UV-selected star-forming galaxies with mean spectroscopic redshift z=2.26 to study the correlation between metallicity and stellar mass at high redshift. Using stellar masses determined from SED fitting to 0.3-8 micron photometry, we divide the sample into six bins in stellar mass, and construct six composite H-alpha+[NII] spectra from all of the objects in each bin. We estimate the mean oxygen abundance in each bin from the [NII]/H-alpha ratio, and find a monotonic increase in metallicity with increasing stellar mass, from 12+log(O/H) = 2.7e9 Msun to 12+log(O/H) = 8.6 for galaxies with = 1e11 Msun. We use the empirical relation between star formation rate density and gas density to estimate the gas fractions of the galaxies, finding an increase in gas fraction with decreasing stellar mass. These gas fractions combined with the observed metallicities allow the estimation of the effective yield y_eff as a function of stellar mass; in constrast to observations in the local universe which show a decrease in y_eff with decreasing baryonic mass, we find a slight increase. Such a variation of metallicity with gas fraction is best fit by a model with supersolar yield and an outflow rate ~4 times higher than the star formation rate. We conclude that the mass-metallicity relation at high redshift is driven by the increase in metallicity as the gas fraction decreases through star formation, and is likely modulated by metal loss from strong outflows in galaxies of all masses. There is no evidence for preferential loss of metals from low mass galaxies as has been suggested in the local universe. [Abridged]Comment: 18 pages, 9 figures, 2 tables; accepted for publication in Ap

c-MYB is a transcriptional regulator of ESPL1/Separase in BCR-ABL-positive chronic myeloid leukemia

Background: Genomic instability and clonal evolution are hallmarks of progressing chronic myeloid leukemia (CML). Recently, we have shown that clonal evolution and blast crisis correlate with altered expression and activity of Separase, a cysteine endopeptidase that is a mitotic key player in chromosomal segregation and centriole duplication. Hyperactivation of Separase in human hematopoietic cells has been linked to a feedback mechanism that posttranslationally stimulates Separase proteolytic activity after imatinib therapy-induced reduction of Separase protein levels. Methods and Results: In search for potential therapy-responsive transcriptional mechanisms we have investigated the role of the transcription factor c-MYB for Separase expression in CML cell lines (LAMA-84, K562, BV-173) and in clinical samples. Quantitative RT-PCR and Western blot immunostaining experiments revealed that c-MYB expression levels are decreased in an imatinib-dependent manner and positively correlate with Separase expression levels in cell lines and in clinical CML samples. RNA silencing of c-MYB expression in CML cell lines resulted in reduced Separase protein levels. Gelshift and ChIP assays confirmed that c-MYB binds to a putative c-MYB binding sequence located within the ESPL1 promoter. Conclusions: Our data suggest that ESPL1/Separase is a regulatory target of c-MYB. Therefore, c-MYB, known to be required for BCR-ABL-dependent transformation of hematopoietic progenitors and leukemogenesis, may also control the Separase-dependent fidelity of mitotic chromosomal segregation and centriole duplication essential for maintenance of genomic stability

Microparticles and exercise in clinical populations

Microparticles (MPs) are shed membrane vesicles released from a variety of cell types in response to cellular activation or apoptosis. They are elevated in a wide variety of disease states and have been previously measured to assess both disease activity and severity. However, recent research suggests that they also possess bioeffector functions, including but not limited to promoting coagulation and thrombosis, inducing endothelial dysfunction, increasing pro‐inflammatory cytokine release and driving angiogenesis, thereby increasing cardiovascular risk. Current evidence suggests that exercise may reduce both the number and pathophysiological potential of circulating MPs, making them an attractive therapeutic target. However, the existing body of literature is largely comprised of in vitro or animal studies and thus drawing meaningful conclusions with regards to health and disease remains difficult. In this review, we highlight the role of microparticles in disease, comment on the use of exercise and dietary manipulation as a therapeutic strategy, and suggest future research directions that would serve to address some of the limitations present in the research to date

Offspring ADHD as a risk factor for parental marital problems: Controls for genetic and environmental confounds

Background: Previous studies have found that child attention-deficit/hyperactivity disorder (ADHD) is associated with more parental marital problems. However, the reasons for this association are unclear. The association might be due to genetic or environmental confounds that contribute to both marital problems and ADHD. Method: Data were drawn from the Australian Twin Registry, including 1,296 individual twins, their spouses, and offspring. We studied adult twins who were discordant for offspring ADHD. Using a discordant twin pairs design, we examined the extent to which genetic and environmental confounds, as well as measured parental and offspring characteristics, explain the ADHD-marital problems association. Results: Offspring ADHD predicted parental divorce and marital conflict. The associations were also robust when comparing differentially exposed identical twins to control for unmeasured genetic and environmental factors, when controlling for measured maternal and paternal psychopathology, when restricting the sample based on timing of parental divorce and ADHD onset, and when controlling for other forms of offspring psychopathology. Each of these controls rules out alternative explanations for the association. Conclusion: The results of the current study converge with those of prior research in suggesting that factors directly associated with offspring ADHD increase parental marital problems

BRANCHLESS TRICHOMES links cell shape and cell cycle control in Arabidopsis trichomes

Endoreplication, also called endoreduplication, is a modified cell cycle in which DNA is repeatedly replicated without subsequent cell division. Endoreplication is often associated with increased cell size and specialized cell shapes, but the mechanism coordinating DNA content with shape and size remains obscure. Here we identify the product of the BRANCHLESS TRICHOMES (BLT) gene, a protein of hitherto unknown function that has been conserved throughout angiosperm evolution, as a link in coordinating cell shape and nuclear DNA content in endoreplicated Arabidopsis trichomes. Loss-of-function mutations in BLT were found to enhance the multicellular trichome phenotype of mutants in the SIAMESE (SIM) gene, which encodes a repressor of endoreplication. Epistasis and overexpression experiments revealed that BLT encodes a key regulator of trichome branching. Additional experiments showed that BLT interacts both genetically and physically with STICHEL, another key regulator of trichome branching. Although blt mutants have normal trichome DNA content, overexpression of BLT results in an additional round of endoreplication, and blt mutants uncouple DNA content from morphogenesis in mutants with increased trichome branching, further emphasizing its role in linking cell shape and endoreplication. © 2011. Published by The Company of Biologists Ltd