Simian malaria in the Brazilian Atlantic forest: first description of natural infection of capuchin monkeys (Cebinae subfamily) by Plasmodium simium

Submitted by Nuzia Santos ([email protected]) on 2016-01-28T15:30:06Z No. of bitstreams: 1 Simian malaria in the Brazilian Atlantic forest.pdf: 6404673 bytes, checksum: 862ebb7bc5da699db4452fc750085236 (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2016-01-28T15:37:11Z (GMT) No. of bitstreams: 1 Simian malaria in the Brazilian Atlantic forest.pdf: 6404673 bytes, checksum: 862ebb7bc5da699db4452fc750085236 (MD5)Made available in DSpace on 2016-01-28T15:37:11Z (GMT). No. of bitstreams: 1 Simian malaria in the Brazilian Atlantic forest.pdf: 6404673 bytes, checksum: 862ebb7bc5da699db4452fc750085236 (MD5) Previous issue date: 2015Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia. Ambulatório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. . Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brasil.Centro de Primatologia do Rio de Janeiro. Rio de Janeiro, RJ, Brasil/Centro Universitário Serra dos Órgãos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia. Ambulatório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. . Rio de Janeiro, RJ, Brasil/Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brasil.Methods: Blood samples from 30 non-human primates belonging to nine species kept in the Primate Centre of Rio de Janeiro were collected. Fragments of spleen and liver from one dead monkey found in the neighborhoods of the Primate Centre were also analysed. Molecular diagnosis was performed by nested PCR (18SSU rRNA) and the amplified fragment was sequenced. Results: Thirty per cent of the captive animals were infected with P. simium and/or P. brasilianum. The dead monkey tested positive for DNA of P. simium. For the first time, Cebinae primates (two specimens of genus Cebus and two of genus Sapajos) were found naturally infected by P. simium. The infection was confirmed by sequencing a small fragment of 18SSU rRNA. Conclusion: The results highlight the possibility of infection by P. simium in other species of non-human primates whose impact could be significant for the malaria epidemiology among non-human primates and, if it becomes clear that this P. simium is able to infect monkeys and, eventually, man, also for the maintenance of transmission of human malaria in the context of a zoonosis in areas under influence of the Atlantic Forest