2 research outputs found
Characteristics of the participants (n = 544) included.
<p>Characteristics of the participants (n = 544) included.</p
Multiplex Blood Reporters for Simultaneous Monitoring of Cellular Processes
Reporters secreted into the conditioned
medium of cells in culture
or into blood in vivo have shown to be useful tools for simple and
noninvasive monitoring of biological processes in real-time. Here,
we characterize the naturally secreted <i>Vargula</i> luciferase
as a secreted blood reporter and show that this reporter can be multiplexed
with the secreted <i>Gaussia</i> luciferase and alkaline
phosphatase for simultaneous monitoring of three different cellular
processes in the same biological system. We applied this system to
monitor the response of three different subsets of glioma cells to
a clinically relevant chemotherapeutic agent in the same well in culture
or animal in vivo. This system could be extended to any field to detect
multiple processes in the same biological system and is amenable for
high-throughput screening to find drugs that affect multiple cellular
populations/phenomena simultaneously