Modulation of the SOD activity in undifferentiated PC12 cells after preincubation with HX, HY and HZ.

<p>Cells were incubated with 200 µM H₂O₂ for 2 h. Huprines were added to the culture 48 h prior to H₂O₂ addition. SOD activity was measured with a SOD activity assay from Fluka. At least three independent experiments were carried out in triplicate. The data are means ± s.e.m. expressed as percentage of control value. *P<0.05 compared with H₂O₂ group (Dunnett's test).</p

Atenuation of H₂O₂-induced cell damage by 0.1 µM and 1 µM concentrations of huprines: HX, HY and HZ in NGF differenciated PC12 cells.

<p>Cells were incubated with 200 µM H₂O₂ for 2 h. Compounds were added to the culture 48 h prior to H₂O₂ addition. Cell viability was assessed by measuring the MTT reduction. At least three independent experiments were carried out in triplicate. The data are means ± s.e.m. expressed as percentage of control value. *P<0.05 compared with H₂O₂ group (Dunnett's test).</p

Effect of huprines pretreatment on NGF differentiated PC12 cells survival after exposure of cells to H₂O₂ (200 µM).

<p>Effect of antagonists mecamylamine (MEC) and atropine (ATR) on huprines protective effect on PC12 cells survival after exposure of the cells to H₂O₂. See Methods for details of cell treatment and conditions. Values are expressed as percentage of protection (mean ± s.e.m.) obtained from at least three independent experiments run in triplicate. *P<0.05 as compared with H₂O₂–treated group; ^bP<0.05 as compared with 1 µM concentration of corresponding drug (Dunnett's test).</p

Effect of CALHM1 on <b>Aβ</b>_1-42- induced cell toxicity.

<p>HEK-293 cells over-expressing CALHM1-WT, CALHM1-G330D and CALHM1-P86L were treated with 2 μM Aβ_1-42 oligomers for 24h. Cell viability was measured by MTT reduction. Data are the mean ± SEM of three independent experiments performed in triplicate. * <i>P</i><0.05 vs untreated cells by student t test.</p

Effect of CALHM1 mutations on Ca²⁺ influx.

<p><b>A</b>. HEK-293 cells transfected with GFP, CALHM1-WT, CALHM1-P86L, CALHM1-R154H, CALHM1-A213T and CALHM1-G330D were loaded with Fura-2 and tested for Ca²⁺ influx into the cytoplasm following the removal of Ca²⁺ from the extracellular media. Time course of Ca²⁺ signals normalized to the value before the addition of Ca²⁺ and expressed as the mean±S.E.M. are shown. <b>B</b>. Mean peak Ca²⁺ signals for the different conditions are shown. Number of cells analysed are given for each condition. * <i>P</i><0.05 <i>vs</i> GFP; ** <i>P</i><0.01 <i>vs</i> GFP, one-way ANOVA and Bonferroni <i>post </i><i>hoc</i>.</p