Technological collaboration : bridging the innovation gap between small and large firms

This paper analyzes how technological collaboration acts as an input to the innovation process and allows small and medium-sized entetprises to bridge the innovation gap with their bigger counterparts. Based on a large longitudinal sample of Spanish manufacturing firms, the results show that though technological collaboration is a useful mechanism for firms of all sizes to improve innovativeness, it is a critical factor for the smallest firms. The impact on this collaboration varies depending on innovation output and type of partner. Specifically, the impact of collaboration in small and medium-sized firms is more significant for product than process innovations. Regarding type of partner, vertical collaboration-with suppliers and clients-has the greatest impact on firm innovativeness, though (his effect is clearer lor medium-sized entetprises than for the smallest firmsThis study has been finaneially supported by projeets SEC]2007-67582-C02-02/ECON and CCG08-UC3M/HUM-4152Publicad

Morphological, physiological and genomic comparisons between diploids and induced tetraploids in Anemone sylvestris L

The objective of this research was to induce mitotic chromosome doubling in Anemone sylvestris L. The mitosis inhibitor oryzalin was directly added to the induction medium at 1, 2, 5, 10 and 15 μM for 8, 10 or 12 weeks of cultivation. Three tetraploid plants (2n = 4x = 32), 0.8% (polyploidization efficiency), were obtained from diploid plants (2n = 2x = 16) in three treatments (1 μM for 10 weeks, 5 μM for 8 weeks and 8 μM for 10 weeks). Ploidy level was confirmed by flow cytometry. Morphological characteristics (e.g. flower diameter, total plant height, leaf area) and chlorophyll content differences between diploid and tetraploid A. sylvestris were observed together with polyphenol content and antioxidant activity. The inter primer binding sites markers were used for evaluation of polymorphism. New genotypes with different morphological and biological characteristics were obtained through somatic polyploidization. The tetraploid plants were stronger, more vigorous and had an early flowering, which is essential for its use as an ornamental plant. The iPBS analysis showed unique amplicons that can be used for the purposes of molecular identification of tetraploid plants of A. sylvestris in the future. The results demonstrate the first report of in vitro induction of tetraploids of A. sylvestris