Evaluation of efficacy of CO2 fractional laser in genitourinary syndrome in menopausal women: a prospective observational study

Background: Objective were to evaluate the efficacy of fractional CO2 laser in treating genito-urinary syndrome (GSM). Methods: We did a prospective observational study on 92 post-menopausal women aged 37-84 years during December 2022-December 2023 in a private clinic of district Kanpur. Fractional CO2 laser (Rosch, vaginal 3600 probe) in three sittings were done for women presenting with GSM at the interval of four weeks as a lunch break therapy. The outcome was studied in terms of visual analogue scale (VAS) having 0 to 10 rating at second, third and sixth months. The statistical analysis was performed using MS excel and GraphPad online statistical calculator. Results: Stress urinary incontinence, early prolapse, urinary incontinence was the commonest presenting complaints among females. Significant improvement was observed in VAS score in each follow up. Conclusions: The vaginal fractional CO2 laser can be used as an effective and safe treatment method in GSM. It is necessary to conduct studies with long-term follow-up

Awareness of geriatric welfare services among rural elderly population

Background: Elderly population suffers from income loss, decreased social role and increased dependence along with physical and mental problems associated with ageing. Geriatric welfare services are aimed to address this problem related to old age. The main focus of the existing geriatric welfare services are below the poverty line elderly population. However, the benefit from these services can be obtained if elderly population have awareness about these welfare services. Main objective of the study was to study the awareness regarding geriatric welfare services among rural elderly population and its association with gender.Methods: Community based cross- sectional study was carried out at field practice area of Rural Health Training Centre, JN Medical College, AMU, Aligarh, Uttar Pradesh, India by using systematic random sampling with probability proportionate to size among 225 rural elderly individuals using pretested and predesigned questionnaire. Data analysis was done using SPSS 20. Chi square test was used. P value <0.05 was considered significant.Results: Among the geriatric welfare services 28.9% were aware of the social security schemes, 84% were aware of the special govt. facilities, 35.6% were aware of the health insurance schemes. Males had significant higher awareness of geriatric welfare services than the females.Conclusions: The study revealed that awareness of geriatric welfare services among rural elderly population was very low that needs to be addressed to improve utilization of these services.

Optimization of Hybrid Power Systems Performance Based on Adaptive Neuro-Fuzzy Inference System

Hybrid Power Systems (HPSs) is a promising solution for the shortages of electricity in several situations. However, HPSs are still facing several problems. These problems are the cost of electrical kilowatt-hour and repetitive breaking in the utility grid with existence varying loads. Besides the problem of non-optimal utilization of available renewable energy resources and the problems associated with the operation of large generators along small loads, which are the high cost of generation and the minimize in lifetime of the generator. This paper presents study and analyze the load profile and power system generation for a selected case. A fuzzy control system based on ANFIS has been proposed to optimize the performance of the HPS. The proposed system has ten ANFIS models, which linked to the outputs of the proposed control system. All models have been trained to achieve the minimum root mean square error (RMSE). The proposed system has been built and simulated using MATLAB

Deriving optimal operational policies for off-stream man-made reservoir considering conjunctive use of surface- and groundwater at the Bar dam reservoir (Iran)

Study region: The off-stream artificial Bar lake, built in 2015 to store the flood flows of the Bar river for domestic and industrial needs and with the objective to intentionally recharge the aquifer, is situated in the Razavi Khorasan province (Iran). Study focus: We present a methodology, based on the combination of a MODFLOW groundwater flow model for estimating seepage rates, and an optimization model, for the management and operation of an artificial reservoir considering surface/groundwater interactions for satisfying 12 Mm3/year of water demand. We simulated the reliable amount of water that can be supplied from the reservoir, considering reservoir seepage, maximizing water supply yields subject to the water supply reliability requirements, and the additional intentional volume of groundwater recharge. New hydrological insights for the region: Our results demonstrate the reliability of conjunctive use of surface-and ground-water in water scarce areas by exploiting reservoir infrastructures with relevant leakage losses, also for creating additional aquifer storage. In such systems, man-induced changes of lake stages can significantly affect the volume of water that seeps through the lakebed. The aquifer, under managed aquifer recharge operations, may then provide the resource not satisfied by the reservoir release, fulfilling 100 % reliability of water supply. The conjunctive use of surface- and ground-water, by improving water security, may open new sustainability views for leaking reservoirs, even if they were not initially designed for increasing aquifer recharge, in many areas worldwide

Management of oesophageal foreign bodies

AbstractObjectiveThe aim of the study was to evaluate the management of foreign bodies in the oesophagus and to determine the association with socioeconomic status.MethodsThis cross-sectional analytical study was carried out in the Department of Ear, Nose and Throat and head and neck surgery of Bahawal Victoria Hospital affiliated with Quaid-i-Azam Medical College, Bahawalpur, Pakistan, between December 2012 and May 2013. The medical records of 34 consecutive cases of foreign body ingestion were searched, and the data were recorded on a questionnaire prepared for this purpose.ResultsThe average age of the patients was 10.38 years; 29 cases (85.2%) were in children under 12 years. There were 18 females (52.9%) and 16 males (47.1%). Thirty patients (88%) presented with a history of dysphagia, and 25 (73.6%) had vomiting. The site of impaction was the post-cricoid region in 22 patients (66%), the lower oesophagus in 5 (15%), the mid-oesophagus in 4 (13%), the posterior pharyngeal wall in 1 (3%) and the pyriform fossa in 1 patient (3%). Coins were the most common foreign body (61.8%). Socioeconomic analysis showed that 18 patients (52.9%) were in the low socioeconomic class, 12 (35.3%) in the middle class and 4 (11.8%) in the upper class.ConclusionThe presence of a foreign body in the oesophagus is a serious condition, and early removal is recommended. Foreign body lodgement is commoner among poor families

Soybean mosaic virus: A successful potyvirus with a wide distribution but restricted natural host range

Taxonomy. Soybean mosaic virus (SMV) is a species within the genus Potyvirus, family Potyviridae that includes almost a quarter of all known plant RNA viruses affecting agriculturally important plants. The Potyvirus genus is the largest of all genera of plant RNA viruses with 160 species. Particle. The filamentous particles of SMV, typical of potyviruses, are about 7,500 Å long and 120 Å in diameter with a central hole of about 15 Å in diameter. Coat protein residues are arranged in helice of about 34 Å pitch having slightly less than 9 subunits per turn. Genome. The SMV genome consists of a single-stranded positive-sense polyadenylated RNA of approximately 9.6 kb with a virus-encoded protein (VPg) linked at the 5\u27 terminus. The genomic RNA contains a single large open reading frame (ORF). The polypeptide produced from the large ORF is processed proteolytically by three viral-encoded proteinases to yield about 10 functional proteins. A small ORF, partially overlapping the P3 cistron, pipo, is encoded as a fusion protein in the N-terminus of P3 (P3N+PIPO). Biological properties. SMV’s host range is restricted mostly to two plant species of a single genus; Glycine max (cultivated soybean) and G. soja (wild soybean). SMV is transmitted by aphids non-persistently and by seeds. Variability of SMV is recognized by reactions on cultivars with dominant resistance (R) genes. Recessive resistance genes are not known. Geographical distribution and economic importance. As a consequence of its seed transmissibility, SMV is present in all soybean growing areas of the world. SMV infections can reduce significantly seed quantity and quality (e.g., mottled seed coats, reduced seed size and viability, and altered chemical composition). Control. The most effective means of managing losses from SMV are planting virus-free seeds and cultivars containing single or multiple R genes. Key attractions. The interactions of SMV with soybean genotypes containing different dominant R genes and understanding functional role(s) of SMV-encoded proteins in virulence, transmission and pathogenicity have been intensively investigated. The SMV-soybean pathosystem has become an excellent model for examining the genetics and genomics of uniquely complex gene-for-gene resistance model in a crop of worldwide importance

A Transcriptional Regulatory Network of \u3cem\u3eRsv3\u3c/em\u3e-Mediated Extreme Resistance against \u3cem\u3eSoybean Mosaic Virus\u3c/em\u3e

Resistance genes are an effective means for disease control in plants. They predominantly function by inducing a hypersensitive reaction, which results in localized cell death restricting pathogen spread. Some resistance genes elicit an atypical response, termed extreme resistance, where resistance is not associated with a hypersensitive reaction and its standard defense responses. Unlike hypersensitive reaction, the molecular regulatory mechanism(s) underlying extreme resistance is largely unexplored. One of the few known, naturally occurring, instances of extreme resistance is resistance derived from the soybean Rsv3 gene, which confers resistance against the most virulent Soybean mosaic virus strains. To discern the regulatory mechanism underlying Rsv3-mediated extreme resistance, we generated a gene regulatory network using transcriptomic data from time course comparisons of Soybean mosaic virus-G7-inoculated resistant (L29, Rsv3-genotype) and susceptible (Williams82, rsv3-genotype) soybean cultivars. Our results show Rsv3 begins mounting a defense by 6 hpi via a complex phytohormone network, where abscisic acid, cytokinin, jasmonic acid, and salicylic acid pathways are suppressed. We identified putative regulatory interactions between transcription factors and genes in phytohormone regulatory pathways, which is consistent with the demonstrated involvement of these pathways in Rsv3-mediated resistance. One such transcription factor identified as a putative transcriptional regulator was MYC2 encoded by Glyma.07G051500. Known as a master regulator of abscisic acid and jasmonic acid signaling, MYC2 specifically recognizes the G-box motif (“CACGTG”), which was significantly enriched in our data among differentially expressed genes implicated in abscisic acid- and jasmonic acid-related activities. This suggests an important role for Glyma.07G051500 in abscisic acid- and jasmonic acid-derived defense signaling in Rsv3. Resultantly, the findings from our network offer insights into genes and biological pathways underlying the molecular defense mechanism of Rsv3-mediated extreme resistance against Soybean mosaic virus. The computational pipeline used to reconstruct the gene regulatory network in this study is freely available at https://github.com/LiLabAtVT/rsv3-network

A Method for Combining Isolates of Phytophthora sojae to Screen for Novel Sources of Resistance to Phytophthora Stem and Root Rot in Soybean

Soybean cultivars with specific single resistance genes (Rps) are grown to reduce yield loss due to Phytophthora stem and root rot caused by the oomycete pathogen Phytophthora sojae. To identify novel Rps loci, soybean lines are often screened several times, each time with an isolate of P. sojae that differs in virulence on various Rps genes. The goal of this study was to determine whether several isolates of P. sojae that differ in virulence on Rpsgenes could be combined into a single source of inoculum and used to screen soybean lines for novel Rps genes. A set of 14 soybean differential lines, each carrying a specific Rps gene, was inoculated with three isolates of P. sojae, which differed in virulence on 6 to 10 Rps genes, individually or in a 1:1:1 mixture. Inoculum containing the 1:1:1 mixture of isolates was virulent on 13 Rps genes. The mixed-inoculum method was used to screen 1,019 soybean accessions in a blind assay for novel sources of resistance. In all, 17% of Glycine max accessions and 11% of G. soja accessions were resistant (≤30% dead plants), suggesting that these accessions may carry a novel Rps gene or genes. Advantages of combining isolates into a single source of inoculum include reduced cost, ability to screen soybean germplasm with inoculum virulent on all known Rps genes, and ease of identifying novel sources of resistance. This study is a precursor to identifying novel sources of resistance to P. sojae in soybean using RXLR effectors