ANÁLISE DA CONTAMINAÇÃO MICROBIOLÓGICA DE LUVAS DE PROCEDIMENTOS UTILIZADAS EM ODONTOLOGIA NA UNIVERSIDADE DE PERNAMBUCO.

The growing number of infections in the dental environment emerges as a warning for the judicious use of personal protective equipment as well as the study of the microbiological safety of these possible carriers of infectious agents. In this context, the objective of this work was to evaluate the presence of microorganisms in procedural gloves used in routine care at dental clinics at the Faculty of Dentistry of the University of Pernambuco (FOP/UPE). In total, 10 boxes of gloves were analyzed, 7 latex and 3 nitrile, from ten different brands for routine use in the dental environment. Two samples were collected from each brand: the first came from a glove whose box was recently opened in a laboratory environment, while the second came from an open box exposed to the routine dental clinical environment, with the gloves not being used in no time by professionals, with the help of a sterilized Swab. The samples were cultivated in dextrosed Sabouraud Agar medium plus chloramphenicol and Mueller-hinton medium and incubated in an oven at a temperature of 34° ± 2°C for 2-3 days for subsequent identification. It is concluded that there was no contamination in the procedural gloves sold, nor were there any contaminations in them after exposure in a clinic, and the biosafety measures used in teaching clinics as well as the good handling of PPE are relevant. Thus, despite the result, controlling the microbial load of procedure gloves used in dental care, as well as rigorous biosafety measures, is essential for oral health care to be truly effective in reducing as much as possible the cross infections.O crescente  número de infecções no ambiente odontológico surge como alerta para o uso criterioso de equipamentos de proteção individual bem como o estudo da segurança microbiológica destes possíveis veículos de agentes infecciosos. Neste contexto, o objetivo deste trabalho foi, avaliar a presença de microrganismos em luvas de procedimentos usados na rotina de atendimento das clínicas odontológicas da Faculdade de Odontologia da Universidade de Pernambuco (FOP/UPE). Ao todo, foram analisadas 10 caixas de luvas, sendo 7 de látex e 3 nitrílicas,  provenientes de dez marcas diferentes de uso rotineiro no ambiente odontológico. De cada marca, foram coletadas duas amostras: a primeira, foi proveniente de uma luva cuja a caixa foi recém aberta em ambiente laboratorial, enquanto que, a segunda, foi oriunda de uma caixa aberta exposta ao ambiente clínico odontológico rotineiro. As luvas avaliadas não foram utilizadas ou manipuladas em nenhum momento pelos profissionais e a coleta das amostras foi realizada  com o auxílio de um Swab esterilizado. As amostras foram cultivadas em meio   Ágar Sabouraud dextrosado acrescido de cloranfenicol e o meio Mueller-hinton e incubada em estufa à temperatura de 34° ± 2°C por 2-3 dias para posterior identificação. Conclui-se que não havia contaminação nas luvas de procedimentos comercializadas, assim como, também não houve contaminação nestas após exposição em clínica. Demonstrando serem pertinentes as medidas de biossegurança empregadas na clínica-escola, bem como a boa manipulação do EPI. Dessa forma, apesar do resultado, o controle da carga microbiana das luvas de procedimentos utilizadas na assistência odontológica, bem como o rigor com as medidas de biossegurança, mostra-se imprescindível para que o atendimento em saúde bucal seja de fato eficaz em reduzir ao máximo as infecções cruzadas.&nbsp

Brazilian Dental Journal

p.177-181The aim of this study was to assess histologically the effect of LLLT (λ 830 nm) on the repair of standardized bone defects on the femur of Wistar albinus rats which were grafted with inorganic bovine bone Gen-ox®. Three randomized groups were studied: group I (control, n=6); group II (Gen-ox®, n=9) and group III (Gen-ox® + LLLT, n=9). The animals were irradiated every 48 h during 15 days; the first irradiation was performed immediately after the procedure. The animals were irradiated transcutaneuosly at four points around the defect. At each point a dose of 4 J/cm2 was given (Ø~0.6 mm, 40 mW) and the total dose per session was 16 J/cm2. The animals were killed by an overdose of general anesthetic 15, 21 and 30 days after surgery. The specimens were routinely processed by embedding in paraffin, serially cut and stained with H&E and Picrosirius and analyzed under light microscopy. The results showed evidence of a more advanced repair in the irradiated group when compared to the non-irradiated groups. The repair of the irradiated group was characterized by both increased bone formation and amount of collagen fibers around the graft within the cavity from the 15th day after surgery, also considering the osteoconductive capacity of the Gen-ox®. We conclude that LLLT had a positive effect on the repair of bone defects implanted with inorganic bovine bone

Photomedicine and Laser Surgery

Texto completo: acesso restrito. p. 382-388Objective: The aim of the present investigation was to assess histologically the effect of LLLT (GaAIAs, 830 nm, 40 mW, CW, (Φ) ~0.6 mm, 16 J/cm2 per session) on the repair of surgical defects created in the femur of the Wistar Albinus rat. The defects were filled to lyophilized bovine bone (Gen-ox®, organic matrix) associated or not to GTR (Gen-derm®). Background data: A major problem on modern Dentistry is the recovery of bone defects caused by trauma, surgical procedures or pathologies. Several types of biomaterials have been used in order to improve the repair of these defects. These materials are often associated to procedures of GTR. Previous studies have shown positive effects of LLLT on the repair of soft tissue wounds, but there are a few on its effects on bone healing. Methods: Surgical bone defects were created in 42 animals divided into five groups: Group I (control, 6 animals); Group II (Gen-ox®, 9 animals); Group III (Gen-ox® + Laser, 9 animals); Group IV (Gen-ox® + Gen-derm, 9 animals); Group V (Gen-ox® + Gen-derm® + Laser, 9 animals). The animals on the irradiated group received 16 J/cm2 per session divided into four points around the defect (4 J/cm2) being the first irradiation immediately after surgery and repeated seven times at every 48 h. The animals were humanly killed after 15, 21, and 30 days. Results: The results of the present investigation showed histological evidence of improved amount of collagen fibers at early stages of the bone healing (15 days) and increased amount of well organized bone trabeculae at the end of the experimental period (30 days) on irradiated animals compared to non irradiated ones. Conclusions: It is concluded that a positive biomodulative effect on the healing process of one defect associated or not to the use of organic lyophilized bone and biological bovine lyophilized membrane on the femur of the rat