Association study for LDA using SNPs within <i>motilin</i> for 1,136 German Holstein cows.

<p>For each SNP, minor allele frequencies (MAF), the MAF allele, odds ratios (OR) with their 95% confidence intervals (CI), polymorphism information content (PIC), and heterozygosity (HET) are given. Furthermore, −log₁₀P-values (P) are given for genotype (gt), allele, among homozygotes (hom) and for genotype after correcting the data for stratification (strat).</p

All identified polymorphisms with their location within <i>motilin</i> (<i>MLN</i>).

*<p>IRD labeled.</p><p>Informative (I) polymorphisms are indicated by a plus sign. The primer pairs used are given with their annealing temperatures (AT) and product sizes (PS).</p

Least-squares means (LSM) with their corresponding standard errors (SE) of the frequency of LDA on the underlying logit scale for the genotypes of the SNPs FN298674:g.90T>C and FN298674:g.1891insG and the contrasts among the LSMs with their test statistics and P-values.

<p>Genotypic means for the frequency of LDA were estimated through LSMs in a mixed logit model correcting for data stratification.</p

Mutation within the bovine <i>motilin (MLN)</i> gene associated with LDA.

<p>Sequence of <i>MLN</i> surrounding the FN298674:g.90T>C SNP for the wildtype (control cow) and the mutated allele (LDA-affected cow) as well as the 3′ end of the predicted <i>MLN</i> promoter, the 5′ start sequence of the non-coding first exon of <i>MLN</i> and the NKX2-5 transcription factor binding site are shown. Furthermore, chromatograms of this section are given for each one of the homozygous wildtype, heterozygous and homozygous mutant individuals.</p

Results of the expression analysis for FN298674:g.90T>C within <i>motilin</i> (<i>MLN</i>).

<p>The mean relative expression levels of <i>MLN</i> with their standard errors are given for the wildtype genotype T/T, the heterozygously (C/T) and the homozygously mutated genotype (C/C) standardized on the mean of the genotype T/T. Furthermore, the F-values and P-values for the differences in mean relative expression levels among the genotype T/T versus the genotypes C/T and C/C as well C/T versus C/C are given. <i>RPL4</i> was used as housekeeping gene.</p