MOESM1 of Structure of a Thermobifida fusca lytic polysaccharide monooxygenase and mutagenesis of key residues

Additional file 1: Figure S1. HPLC chromatograms of the full range of glucose gluconolactone ratios A) that demonstrate sample neutral/oxidized product ratio using B) linear standard curves for neutral and C1 oxidized monosaccharides for quantification of LPMO products

24h digestion of pretreated corn stover with individual and combination of both enzymes.

<p>Error bars represent standard deviation of the triplicates.</p

HPLC chromatograms of xylan digestion by individual XynII, XlnD and combination of both enzymes after 5 min incubation.

<p>(a) xylan digestion by XynII; (b) xylan digestion by XlnD; (c) Xylan digestion by combination of XynII and XlnD.</p

Xylanase genes expressed in <i>Y. lipolytica</i> using the secretion vector pYLSC1.

<p>Xylanase genes expressed in <i>Y. lipolytica</i> using the secretion vector pYLSC1.</p

Growth of transformant Yl[ThXynII] and Yl[AnXlnD] on xylan mineral plate the plates were stained with 0.2% Congo red and destained by 1M NaCL.

<p>Growth of transformant Yl[ThXynII] and Yl[AnXlnD] on xylan mineral plate the plates were stained with 0.2% Congo red and destained by 1M NaCL.</p

SDS-PAGE analysis of xylanases produced by <i>Y. lipolytica</i> transformants.

<p>SDS-PAGE analysis of xylanases produced by <i>Y. lipolytica</i> transformants.</p

Xylan conversion by individual XynII, XlnD and combination of both enzymes after 5 min incubation.

<p>Error bars represent standard deviation of the triplicates.</p

Growth of xylanase transformants in liquid medium with birchwood xylan as the carbon source.

<p>(a) Empty vector, (b) Yl[ThXynII], (c) Yl[AnXlnD], and (d) Yl[ThXynII] and Yl[AnXlnD].</p

HPLC chromatogram of the digestion of mixed xylo-oligomers (degree of polymerization up to 6) by XlnD at 0 h (a), or 2 h (b).

<p>HPLC chromatogram of the digestion of mixed xylo-oligomers (degree of polymerization up to 6) by XlnD at 0 h (a), or 2 h (b).</p

MOESM1 of Natural diversity of glycoside hydrolase family 48 exoglucanases: insights from structure

Additional file 1. Additional table and figure