Highly Efficient Quenching of Nanoparticles for the Detection of Electron-Deficient Nitroaromatics

Reported herein is the highly efficient quenching of fluorescent organic nanoparticles by 2,4-dinitrotoluene (DNT) and 2,4,6-trinitrotoluene (TNT). These fluorescent nanoparticles are formed from the hydrophobic collapse of fluorescent polymer chains, and display quenching efficiencies that are in line with the highest reported literature values. Moreover, the fluorescent quenching occurs only for the fluorescent nanoparticles, and not for the precursor polymer solutions, which display marked insensitivity to the presence of nitroaromatics. This aggregation-dependent fluorescent quenching has numerous applications for the detection of small-molecule electron-deficient analytes

Molecular dynamics simulations of the transformation of carbon peapods into double-walled carbon nanotubes

The transformation of carbon peapods (encapsulated fullerenes in nanotubes) into doublewalled nanotubes was studied using molecular dynamics simulation. The simulations reproduce the two main trends known experimentally: the production of low-defect nanotubes and the templating effect of the outer tube. The process involves a low-temperature polymerization of the fullerenes followed by higher temperature self-assembly into a tube. Modelling of this second stage is made possible by the use of the Environment-Dependent Interaction Potential, a large number of atoms and long-time annealing. Analysis shows that the outer tube acts as a container for the self-assembly process, analogous to previous simulations and experiments in which free surfaces, either external or internal, template the formation of highly ordered sp2 phases

Post Aerobic Digestion (PAD) is a Solids Sidestream Nutrient Removal Process that Utilizes Native Carbon: Performance and Key Operational Parameters from Two Full-Scale PAD Reactors

Nutrient management is a critical issue for Water Resource Recovery Facilities, and sidestream treatment technologies to reduce nutrient loads often focus on liquid sidestreams and require external carbon sources. Post aerobic digestion (PAD), whereby an aerobic digester follows an anaerobic digester, treats a solids stream (i.e., anaerobic digester effluent) to reduce nitrogen loads. Volatile solids reduction occurs in this process with residual organic compounds serving as a native carbon source for denitrification. While this process has been evaluated at the lab-scale, information on operational parameters that affect full-scale performance is limited. We evaluated two separate full-scale PAD reactors to determine process performance and key operational parameters. During healthy operation, ammonia removal was greater than 90%, total inorganic nitrogen removal was greater than 80%, and volatile solids reduction was approximately 10%. Low SRT values of 7–10 days, pH ranges of 6.0–7.5, temperatures from 29–38 °C (85–100 °F), and negative ORP values resulted in good performance

Assimilation of All-Weather GMI and ATMS Observations into HWRF

We propose a novel Bayesian Monte Carlo Integration (BMCI) technique to retrieve the profiles of temperature, water vapor, and cloud liquid/ice water content from microwave cloudy measurements in the presence of TCs. These retrievals then can either be directly used by meteorologists to analyze the structure of TCs or be assimilated to provide accurate initial conditions for the NWP models. The technique is applied to the data from the Advanced Technology Microwave Sounder (ATMS) onboard Suomi National Polar-orbiting Partnership (NPP) and Global Precipitation Measurement (GPM) Microwave Imager (GMI)

Importance of the pleckstrin homology domain of dynamin in clathrin-mediated endocytosis

AbstractThe GTPase dynamin plays an essential role in clathrin-mediated endocytosis [1–3]. Substantial evidence suggests that dynamin oligomerisation around the necks of endocytosing vesicles and subsequent dynamin-catalysed GTP hydrolysis is responsible for membrane fission [4,5]. The pleckstrin homology (PH) domain of dynamin has previously been shown to interact with phosphoinositides, but it has not been determined whether this interaction is essential for dynamin's function in endocytosis [6–9]. In this study, we address the in vivo function of the PH domain of dynamin by assaying the effects of deletions and point mutations in this region on transferrin uptake in COS-7 fibroblasts. Overexpression of a dynamin construct lacking its entire PH domain potently blocked transferrin uptake, as did overexpression of a dynamin construct containing a mutation in the first variable loop of the PH domain. Structural modelling of this latter mutant suggested that the lysine residue at position 535 (Lys535) may be critical in the coordination of phosphoinositides, and indeed, the purified mutant no longer interacted with lipid nanotubes. Interestingly, the inhibitory phenotype of cells expressing this dynamin mutant was partially relieved by a second mutation in the carboxy-terminal proline-rich domain (PRD), one that prevents dynamin from binding to the Src homology 3 (SH3) domain of amphiphysin. These data demonstrate that dynamin's interaction with phosphoinositides through its PH domain is essential for endocytosis. These findings also support our hypothesis that PRD–SH3 domain interactions are important in the recruitment of dynamin to sites of endocytosis

Radio frequency component and method of making same

An electrical component and a method of constructing it are disclosed. The component includes a hollow tubular structure. The structure includes a series of axially spaced apart rings and at least one outer perimeter housing member. The housing member interconnects the rings for defining an internal configuration of the hollow tubular structure for electrical purposes. The rings and the housing member each include inter-engageable elements for helping secure mechanically the rings and housing member together to facilitate final assembly of the electrical component

Could automated vehicles reduce transport energy?

Transport energy use and carbon emissions continue to rise, but both need to be drastically reduced. Conventional proposed solutions, all already used to some extent, include a shift to low carbon transport fuels, major improvements in vehicular fuel efficiency, and modal shift. However, their impact has been marginal. This paper instead examines the extent to which fully automated vehicles could contribute to the environmental sustainability of global passenger transport. Fully automated vehicles were found to lead to either an increase or, at best, a slight decrease in energy use and greenhouse gas emissions, and so will be of marginal use at best for reducing emissions in a business-as-usual world. Reasons for this conclusion are first, their potentially lower time and money costs would tend to increase vehicular travel, offsetting any energy efficiency gains, and second, that they face serious problems that could delay or even prevent their widespread introduction

Approximating Input Data to a Snowmelt Model Using Weather Research and Forecasting Model Outputs in Lieu of Meteorological Measurements

Forecasting the timing and magnitude of snowmelt and runoff is critical to managing mountain water resources. Warming temperatures are increasing the rain–snow transition elevation and are limiting the forecasting skill of statistical models relating historical snow water equivalent to streamflow. While physically based methods are available, they require accurate estimations of the spatial and temporal distribution of meteorological variables in complex terrain. Across many mountainous areas, measurements of precipitation and other meteorological variables are limited to a few reference stations and are not adequate to resolve the complex interactions between topography and atmospheric flow. In this paper, we evaluate the ability of the Weather Research and Forecasting (WRF) Model to approximate the inputs required for a physics-based snow model, iSnobal, instead of using meteorological measurements, for the Boise River Basin (BRB) in Idaho, United States. An iSnobal simulation using station data from 40 locations in and around the BRB resulted in an average root-mean-square error (RMSE) of 4.5 mm compared with 12 SNOTEL measurements. Applying WRF forcings alone was associated with an RMSE of 10.5 mm, while including a simple bias correction to the WRF outputs of temperature and precipitation reduced the RMSE to 6.5 mm. The results highlight the utility of using WRF outputs as input to snowmelt models, as all required input variables are spatiotemporally complete. This will have important benefits in areas with sparse measurement networks and will aid snowmelt and runoff forecasting in mountainous basins

Computational Microwave Imaging Using 3D Printed Conductive Polymer Frequency-Diverse Metasurface Antennas

A frequency-diverse computational imaging system synthesized using three-dimensional (3D) printed frequency-diverse metasurface antennas is demonstrated. The 3D fabrication of the antennas is achieved using a combination of PolyLactic Acid (PLA) polymer material and conductive polymer material (Electrifi), circumventing the requirement for expensive and time-consuming conventional fabrication techniques, such as machine milling, photolithography and laser-etching. Using the 3D printed frequency- diverse metasurface antennas, a composite aperture is designed and simulated for imaging in the K-band frequency regime (17.5-26.5 GHz). The frequency-diverse system is capable of imaging by means of a simple frequency-sweep in an-all electronic manner, avoiding mechanical scanning and active circuit components. Using the synthesized system, microwave imaging of objects is achieved at the diffraction limit. It is also demonstrated that the conductivity of the Electrifi polymer material significantly affects the performance of the 3D printed antennas and therefore is a critical factor governing the fidelity of the reconstructed images.Comment: Original manuscript as submitted to IET Microwaves, Antennas & Propagation (2017). 17 pages, 8 figure