Heat map comparing clusters of genes present (red) or absent (black) in each <i>E</i>. <i>coli</i> genome.

<p>SCB34 and RS218 are highlighted by the arrows, among 33 additional representative <i>E</i>. <i>coli</i> strains. The dotted-line boxes highlight the clade that includes SCB34, and the names of the two additional <i>E</i>. <i>coli</i> strains with the most similar protein-coding sequences within the clade. A hierarchical clustering of the genes found in all genomes is found at the left of the heat map, while a tree based on hierarchical clustering of genomes is shown at the top of the heat map.</p

Virulence in newborn rats after inoculation with neonatal <i>E</i>. <i>coli</i> isolates SCB34 or RS218.

<p><b>1A.</b> Animals were inoculated orally and survival post-inoculation is shown (data from three separate experiments, n = 10 animals per group, per experiment). <i>*p<0</i>.<i>05 SCB34 vs</i>. <i>DH5α</i>, <i>**p<0</i>.<i>03 RS218 vs</i>. <i>DH5α</i>. <b>1B.</b> Survival after intraperitoneal inoculation (data from two separate experiments, n = 10 animals per group, per experiment). <i>‡p <</i> .<i>001 RS218 vs</i>. <i>SCB34 or RS218 vs</i>. <i>DH5α</i>.</p

Invasion of intestinal T84 epithelial cells by SCB34 and RS218.

<p>The nonpathogenic laboratory strain DH5α was used as control. SCB34 demonstrated the highest invasion ability among the three strains. *SCB34 <i>vs</i>. RS218 (p≤0.03); **SCB34 <i>vs</i>. and DH5α (p<0.001); and ***RS218 <i>vs</i>. DH5α (p<0.01).</p

Growth curves of neonatal <i>E</i>. <i>coli</i> clinical isolates RS218 and SCB34.

<p>Growth curves performed in (4A) LB media and (4B) tissue culture media (TCM) by plate counts. Each plot shows mean CFU values in triplicate samples per time point of each individual clinical isolates. Error bars represent one standard deviation.</p

Representative pathotypes and number of unique genes in 16 strains selected among all strains included in Fig 6.

<p>Representative pathotypes and number of unique genes in 16 strains selected among all strains included in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0189032#pone.0189032.g006" target="_blank">Fig 6</a>.</p

Transcytosis of <i>E</i>. <i>coli</i> clinical isolates across polarized intestinal epithelial cells.

<p>*p<0.05 RS218 vs. DH5−α; † p<0.05 SCB34 vs. DH5−α; ‡ p<0.05 SCB34 vs. RS218. Model−calculated mean of cumulative log_e (CFU+1) values over time are shown. Error bars reflect 95% Bayesian confidence intervals. Points were jittered to improve clarity (combined data from three separate experiments).</p

Comparison of gene distribution among SCB34, RS218, and DH5α.

<p>The Venn diagram shows genes that are common or unique genes in each isolate. The unique proteins have been determined by comparison between the 3 strains.</p