MOESM3 of Reduced antiretroviral drug efficacy and concentration in HIV-infected microglia contributes to viral persistence in brain

Additional file 3.  Brain tissue (70–100 mg) from all BLT HIV-infected and uninfected animals was used to extract RNA. The RNA was used to quantify host genes (A) human CD163 (B) human IP10 (C) human MX2 and (D) mouse f4/80

MOESM4 of Reduced antiretroviral drug efficacy and concentration in HIV-infected microglia contributes to viral persistence in brain

Additional file 4.  Differentiated THP-1 cells were infected with HIV-1 YU-2 and SF162. DNA was extracted from infected cells and used to establish the integration assay (A). Host genes expression from patients 1 and 2 compared to uninfected controls measured by quantitative RT-PCR for, (B) CD163, (C) IL6, (D) TNFA, (E) APOBEC3G, (F) MX2, (G) MX1 and (H) BST2

Patient ACT 95387.

<p>Patient ACT 95387.</p

Patient GOL041.

<p>Patient GOL041.</p

Patient HTM 399.

<p>Patient HTM 399.</p

Patient ACT 87524.

<p>Patient ACT 87524.</p

Patients' characteristics.

<p>Legend to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100452#pone-0100452-t002" target="_blank">Table 2</a> to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100452#pone-0100452-t007" target="_blank">7B</a>: The CTL epitopes that should be recognized according to the HLA alleles are defined using the HXB2 reference. The observed corresponding epitopes in the different samples are described either for RNA or DNA. The dates are the dates of sampling.</p><p>« Id » indicates that the epitope observed is identical to the HxB2 reference. The numbers in parenthesis are the theorical MHC IC₅₀ (nM) according to simulation of affinity between the epitopes and the HLA alleles. The numbers in parenthesis and italic represent percentages of RT variants determined by UDPS.</p