The unadjusted frequency of macrovascular disease, stratified according to age and ethnicity

<p><b>Copyright information:</b></p><p>Taken from "The management of diabetes in indigenous Australians from primary care"</p><p>http://www.biomedcentral.com/1471-2458/7/303</p><p>BMC Public Health 2007;7():303-303.</p><p>Published online 25 Oct 2007</p><p>PMCID:PMC2174478.</p><p></p> I = Indigenous, NI = Non-Indigenous, TN = total NEFRON cohort. Error bar denotes 95% confidence interva

Geographic distribution of Australian practices enrolling Indigenous patients in the NEFRON study

<p><b>Copyright information:</b></p><p>Taken from "The management of diabetes in indigenous Australians from primary care"</p><p>http://www.biomedcentral.com/1471-2458/7/303</p><p>BMC Public Health 2007;7():303-303.</p><p>Published online 25 Oct 2007</p><p>PMCID:PMC2174478.</p><p></p

The unadjusted frequency of (a) CKD, stratified according to age and ethnicity

<p><b>Copyright information:</b></p><p>Taken from "The management of diabetes in indigenous Australians from primary care"</p><p>http://www.biomedcentral.com/1471-2458/7/303</p><p>BMC Public Health 2007;7():303-303.</p><p>Published online 25 Oct 2007</p><p>PMCID:PMC2174478.</p><p></p> Crosshatch denotes patients with an elevated ACR, shading denotes patients with an eGFR < 60 mol/min/1.73 m, I = Indigenous, NI = Non-Indigenous, TN = total NEFRON cohort. Error bar denotes 95% confidence interval for CKD

The frequency of an elevated urinary ACR (± 95% CI), stratified according to duration of diabetes

<p><b>Copyright information:</b></p><p>Taken from "The management of diabetes in indigenous Australians from primary care"</p><p>http://www.biomedcentral.com/1471-2458/7/303</p><p>BMC Public Health 2007;7():303-303.</p><p>Published online 25 Oct 2007</p><p>PMCID:PMC2174478.</p><p></p> Dark squares denote Indigenous patients, white triangles denotes the total NEFRON cohort

Early farmers from across Europe directly descended from Neolithic Aegeans—Hofmanová et al., 3D figure S4

Three-dimensional PCA of modern reference populations and projected ancient individuals. The Greek and Anatolian samples reported here cluster tightly with other European farmers close to modern-day Sardinians, however, are clearly distinct from previously published Caucasian hunter-gatherers. This excludes the latter as potential ancestral source population for early European farmers and suggests strong genetic structure in hunter-gatherers of southwest Asia. A two-dimensional version including the legend explaining the colour code are part of Figure 2 of the main text

Sequences for Tyr2R and Tyr2F gRNAs.

<p>Sequences for Tyr2R and Tyr2F gRNAs.</p

Summary of off-target scores for selected gRNAs.

<p>Off-targets numbers as determined by Cas-OFFinder [<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007503#pgen.1007503.ref009" target="_blank">9</a>] for Tyr2F, Tyr2R used in our experiment and Schaeffer (15).</p

Sequences of primers flanking expected on-target sites for Tyr2R and Tyr2F.

<p>Sequences of primers flanking expected on-target sites for Tyr2R and Tyr2F.</p

Analysis of CRISPR off-targets by whole genome sequencing.

<p>(a) Experimental design: Four sets of C57BL/6N parents gave rise to 9 control embryos (3 “no injection”, 3 “sham injection” with water only and 3 “Cas9 only”), and 10 treated embryos (5 were injected with Cas9 and Tyr2F gRNA and 5 were injected with Cas9 and Tyr2R gRNA). (b) Whole genome sequencing: All 27 mice / embryos were subjected to whole genome sequencing with median depth 39.5x and an average of 3.4% of bases with read depth less than 11x. (c) Variant calling and filtering: starting from the joint variant call (<i>bcftools mpileup + bcftools call</i>), a sequence of filter steps were performed to detect <i>de novo</i> mutations and remove likely false positives arising from low-level parental mosaicism and alignment errors at repeat regions. Parental-noise: alternate-allele reads present in either parent. Cross-noise: alternate reads from all other (non-parental) samples. (d) Filtered SNV and Indel counts are not significantly different within control groups, within treatment groups, or between control and treatment groups.</p

Concentration of reagent injected for each experimental group.

<p>Concentration of reagent injected for each experimental group.</p