a Health service resource use and cost chart.

<p>B. Health service resource use and cost chart.</p

NHS summary costs.

<p><b>NB:</b> Summary of mean total NHS financial cost per patient (in time specified) and mean total financial cost to patient (in time specified).</p>*<p>P<0.001.</p><p>Average difference in <b>NHS cost</b> per patient per year is £89.06 range £+47.50 to −150.75 in favour of the intervention.</p><p>Average difference in <b>Patient cost</b> per year is £5.21 range £3.50–6.91 in favour of the intervention.</p><p><b>NB.</b> Koinberg (2009) costs originally published in Euros, converted to Sterling using Jan 2006 exchange rates (0.688 Euros to the pound) (<a href="http://www.x-rates.com/cgi-bin/hlookup.cgi" target="_blank">http://www.x-rates.com/cgi-bin/hlookup.cgi</a>).</p

Logic model of traditional and Patient Initiated Clinic appointment systems.

<p>Logic model of traditional and Patient Initiated Clinic appointment systems.</p

Clinical outcomes chart.

<p>Clinical outcomes chart.</p

Quality of studies in included.

<p>Quality of studies in included.</p

Characteristics of included studies.

<p>Characteristics of included studies.</p

Identification and selection of studies.

<p>Identification and selection of studies.</p

Additional file 1: of The anti-proliferative and anti-inflammatory response of COPD airway smooth muscle cells to hydrogen sulfide

Figure S1. Immunohistochemistry staining of CSE, CBS and MPST in bronchial biopsies from non-smokers, smokers and COPD patients. Photomicrographs showing representative photomicrographs of cystathionine-γ-lysase (CSE), cystathionine-β-synthase (CBS) and 3-mercaptopyruvate sulphur transferase (MPST) staining in the bronchial mucosa from control non-smokers, control smokers with normal lung function and mild/moderate COPD patients. Immune-stained airway smooth muscle cells are indicated by brown staining. Results are representative of those from 13 non-smokers, 14 smokers with normal lung function, 15 mild/moderate COPD patients. Calibration bar represents 20 μm. Graphical representation of the results are shown in the right hand panels. (JPG 332 kb

Additional file 2: of The anti-proliferative and anti-inflammatory response of COPD airway smooth muscle cells to hydrogen sulfide

Figure S2. Further examples of the effect of the H2S donor, NaSH on CBS and MPST protein expression, and activation of extracellular signal–regulated kinase (ERK)–1/2 and p38 mitogen-activated protein kinase (MAPK) in human ASM cells from non-smokers, smokers and COPD patients. ASM cells were incubated with FCS (2.5%) for 1 h and NaSH (100 μM) was added for another 24 h. CBS, MPST (A), Total and phospho–ERK-1/2, total and phospho-p38 and β-actin (B) were detected by Western blotting. (JPG 212 kb

5T2MM bearing mice have increased bone loss in the lumbar vertebrae and calvariae.

<p>A. Photomicrographs of the vertebrae from naïve and 5T2MM bearing mice. Tumour infiltration is identified with black arrows. B. The proportion of trabecular bone area in the vertebrae from naïve and 5T2MM-bearing mice. C. Photomicrographs of the calvariae of naïve and 5T2MM bearing mice. Medullary spaces are identified with black arrows, and OC with yellow arrows. Non tumour-containing spaces are shown. D. The proportion of medullary area as a percentage of total tissue area in naïve and 5T2MM-bearing mice. E. Distribution of medullary spaces in naïve and 5T2MM-bearing mice, showing significant differences in distribution (KS test, p<0.02). Chi-square analysis demonstrated that 5T2MM-bearing mice had significantly fewer, smaller spaces and more, larger spaces than naïve mice (p<0.05). Data = mean± S.E.M. (B and D).</p