GO analysis of regulated proteins by LPS.

<p>(A) Upregulated proteins by LPS treatment belonged to the GO classes: respiratory electron transport chain and generation of precursor metabolites and energy processes. (B) Downregulated proteins especially belonged to the GO class protein glycosylation and to a smaller degree lipid metabolic processes. (C and D) Schematic overview of the distribution of upregulated (C) and downregulated (D) proteins in different GO classes represented here by gene name. Abbreviations: please see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0159747#pone.0159747.s001" target="_blank">S1 Table</a>.</p

STAT1 expression measured by qPCR and SILAC-MS.

<p>(A) STAT1 gene expression measured by qPCR in 3T3-L1 cells incubated with LPS and resveratrol. (B) IFIT1 protein expression measured by SILAC-MS in 3T3-L1 cells incubated with LPS and resveratrol.</p

Effect of resveratrol and/or LPS on adiponectin expression.

<p>(A) Adiponectin expression was measured by qPCR analysis in epididymal and subcutaneous adipose tissue (n = 9–10 per group). (B) Plasma values of adiponectin (n = 9–10 per group). Data are presented as means ± SEM. *P < 0.05, **P < 0.01 according to unpaired t-test.</p

Differentiation of 3T3-L1 cells.

<p>Lipoprotein lipase (lpl) expression during the differentiation process of 3T3-L1 cells.</p

Thirty most downregulated proteins.

<p>Thirty most downregulated proteins.</p

IFIT1 expression in whole adipose tissue measured by qPCR and in 3T3-L1 cells measured by and SILAC-MS.

<p>(A) Mice treated with LPS for 28 days showed increased gene expression of IFIT1, which was ameliorated by resveratrol delivered through the diet. (B) IFIT1 protein expression measured by SILAC-MS in 3T3-L1 cells incubated with LPS and resveratrol.</p

LPS induce enhanced GSIS and is reversed by resveratrol.

<p>(A) Oral glucose tolerance test (OGTT) in mice treated with LPS and/or resveratrol (n = 13–14 per group). (B) Area under the curve of (A) for each treatment group. (C) Insulin concentrations 0 and 15 minutes after oral administration of a glucose dose (n = 12–15 per group). (D) The insulinogenic index (delta_0-15Insulin/delta_0-15Glucose) was calculated for each treatment group (n = 12–15). Data are presented as means ± SEM. Means with different superscript letters are significantly different at P < 0.05 according to post-hoc ANOVA.</p

Resveratrol only reduces LPS-induced inflammation in epididymal adipose tissue.

<p>(A) Total leukocyte count of whole blood in mice treated with LPS and/or resveratrol (n = 10 per group). (B) Spleen weights as percentage of body weight (n = 10 per group). (C, D, E) qPCR analyses of gene expression of the pro-inflammatory cytokines TNFa, Il1b and the macrophage marker CD14 in liver (C; n = 8–10 per group), subcutaneous (D; n = 10 per group) and epididymal adipose tissue (E; 7–10 per group) and skeletal muscle (F; n = 9–10 per group). Data are presented as means ± SEM. Means with different superscript letters are significantly different at P < 0.05 according to post-hoc ANOVA.</p