Negative binomial regression models to assess the relationship between Integrated HIV DNA and the frequency of CD4⁺ T cells expressing PD-1 and/or TIGIT and/or LAG-3.

<p>Negative binomial regression models to assess the relationship between Integrated HIV DNA and the frequency of CD4⁺ T cells expressing PD-1 and/or TIGIT and/or LAG-3.</p

Co-expression of PD-1, TIGIT and LAG-3 is a marker of HIV persistence during ART.

<p>(A) Frequency of CD4⁺ T cells co-expressing PD-1 and/or TIGIT and/or LAG-3 (PD-1/TIGIT/LAG-3 triple–(P-T-L-), PD-1 single + (P+), TIGIT single + (T+), LAG-3 single + (L+), PD-1/TIGIT double + (P+T+), TIGIT/LAG-3 double + (T+L+), PD-1/LAG-3 double + (P+L+) and PD-1/TIGIT/LAG-3 triple + (P+T+L+)) determined by Boolean gating in cohort 1 (n = 48). Horizontal bars indicate median values with interquartile ranges. (B) Venn diagram showing the pattern of co-expression of PD-1, TIGIT and LAG-3. (C), (D), (E), (F) Associations between the frequency of CD4⁺ T cells harboring integrated HIV DNA and the frequency of CD4⁺ T cells expressing none of these markers (triple–), PD-1 and TIGIT (double +), TIGIT and LAG-3 (double +) and PD-1 and TIGIT and LAG-3 (triple +), respectively. P values were obtained from negative binomial regression analysis. Effect sizes for the associations are as follows: (C) 0.69-fold-change in integrated HIV DNA for 1 point increase in percentage of PD-1/TIGIT/LAG-3 triple—CD4⁺ T cells, (D) 1.18-fold-change in integrated HIV DNA for 1 point increase in percentage of PD-1/TIGIT double + CD4⁺ T cells, (E) 1.30-fold-change in integrated HIV DNA for 1 point increase in percentage of TIGIT/LAG-3 double + CD4⁺ T cells and (F) 1.94-fold-change in integrated HIV DNA for 1 point increase in percentage of PD-1/TIGIT/LAG-3 triple + CD4⁺ T cells. Open circles represent the limit of detection in the negative samples (based on cell input).</p

Clinical demographics of the cohorts.

<p>Clinical demographics of the cohorts.</p

PD-1, TIGIT and LAG-3 identify HIV-infected cells in distinct memory CD4⁺ T-cell subsets during ART.

<p>(A), (B), (C) Frequencies of memory CD4⁺ T-cell subsets (naïve (T_N), central memory (T_CM), transitional memory (T_TM), effector memory (T_EM) and terminally differentiated (T_D)) expressing PD-1, TIGIT or LAG-3, respectively. Horizontal bars indicate median values with interquartile ranges. (D), (E), (F) Frequencies of cells harboring integrated
HIV DNA in T_CM, T_TM and T_EM CD4⁺ T-cell subsets sorted based on their expression of PD-1 (n = 12), TIGIT (n = 9) or LAG-3 (n = 7), respectively. Results are expressed as the HIV copy number in million cells of a
given subset. P values were obtained from negative binomial regression analysis. Significant differences (p<0.05) are designated by a p value in bold. Open circles represent the limit of detection in the negative samples (based on cell input).</p

Negative binomial regression models to assess the relationship between integrated HIV DNA and IC expression on CD4⁺ T cells.

<p>Negative binomial regression models to assess the relationship between integrated HIV DNA and IC expression on CD4⁺ T cells.</p

Co-expression of PD-1, TIGIT and LAG-3 identifies HIV-infected cells during ART.

<p>(A) Pie chart representing the frequencies of memory CD4⁺ T cells co-expressing PD-1 and/or TIGIT and/or LAG-3 in cohort 1 (n = 48). Coloured bars on the right side designate categories of ICs expressing cells: Triple -: PD-1/TIGIT/LAG-3 triple—in blue; single +: PD-1 single +, TIGIT single +, LAG-3 single + in green; double +: PD-1/TIGIT double +, TIGIT/LAG-3 double +, PD-1/LAG-3 double + in orange and triple +: PD-1/TIGIT/LAG-3 triple + in red. (B) Frequency of memory CD4⁺ T cells harboring integrated HIV DNA represented as a fold change over frequency in total CD4⁺ T cells. Mean values and standard deviations from 5 independent donors are represented (n = 5). (C) Frequency of cells harboring inducible msRNA measured by TILDA in memory CD4⁺ T cells expressing any (i.e. at least one) versus none of PD-1, TIGIT or LAG-3 (mLPT+ and mLPT- respectively). P value and fold-difference were obtained from a maximum likelihood model.</p

PD-1, TIGIT and LAG-3 are associated with virological markers of HIV persistence during ART.

<p>(A) Expression of 7 ICs on CD4⁺ T cells in individuals receiving suppressive ART (n = 48). Data is represented as percentage of CD4⁺ T cells and horizontal bars indicate median values with interquartile ranges. (B) Size of the HIV reservoir measured by integrated HIV DNA, total HIV DNA, 2-LTR circles represented as copies per million CD4⁺ T cells and cell-associated US HIV RNA represented as copies per million copies of 18S. Horizontal bars indicate median values with interquartile ranges and open circles represent the limit of detection in the negative samples (based on cell input). (C), (D), (E) Associations between the frequency of CD4⁺ T cells harboring integrated HIV DNA and the frequency of CD4⁺ T cells expressing PD-1, TIGIT and LAG-3, respectively. P values were obtained from negative binomial regression analysis. Effect sizes for the associations are as follows: (C) A 2-fold increase in the percentage of PD-1⁺ CD4⁺ T cell was associated with 1.43-fold increase in the frequency of CD4⁺ T cell harboring integrated HIV DNA, (D) a 2-fold higher percentage of TIGIT⁺ CD4⁺ T cell was associated with 1.91-fold higher integrated HIV DNA and (E) a 2-fold higher percentage of LAG-3⁺ CD4⁺ T cell was associated with 1.62-fold higher integrated HIV DNA. Open circles represent the limit of detection in the negative samples (based on cell input).</p