Translocation breakpoints mapping and genomic features at the breakpoints.

<p>Translocation breakpoints mapping and genomic features at the breakpoints.</p

<i>RAD50</i> gene conditional inactivation in <i>L</i>. <i>infantum</i>.

<p><b>(A)</b> Schematic representation of the <i>RAD50</i> locus in <i>L</i>. <i>infantum</i> before and after integration of the inactivation cassettes blasticidin-S deaminase (5’-<i>BLAST-3’</i>), puromycin acetyltransferase (5’-<i>PURO-3’</i>) and transfection construct Psp-<i>NEO</i>-<i>RAD50</i>. S, SacI restriction sites. <b>(B, C)</b> Southern blot analysis with genomic DNAs digested with SacI were hybridized with probes covering either the 5’ flanking region of <i>RAD50</i> <b>(B)</b> or the <i>RAD50</i> ORF <b>(C). (D)</b> PCR analysis with primers set aa’ and bb’ the chromosomal copies of the <i>MRE11</i> and <i>RAD50</i> genes respectively. Lanes: 1, <i>L</i>.<i>infantum</i> WT; 2, WT Psp-<i>NEO-RAD50</i>; 3, WT Rev Psp-<i>NEO</i>-<i>RAD50</i>; 4, <i>RAD50</i>^<i>-/-</i> Psp-<i>NEO</i>-<i>RAD50</i>; 5, <i>RAD50</i>^<i>-/-</i> Rev Psp-<i>NEO</i>-<i>RAD50</i> grown for 55 passages in absence of G418; 6, <i>MRE11</i>^<i>-/-</i> and 7, <i>MRE11</i>^<i>-/-</i><i>RAD50</i>^<i>-/-</i>.</p

Translocation between chromosome 12 and 06 in <i>L</i>. <i>infantum MRE11</i>^<i>-/-</i><i>RAD50</i>^<i>-/-</i> cells.

<p><b>(A)</b> Schematic representation of the translocation T 12–06 between chromosomes 12 and 06. <b>(B)</b> <i>L</i>. <i>infantum</i> chromosomes were separated by pulsed-field gel electrophoresis, transferred on membranes then hybridized with probes from LinJ.12.0671 (■), LinJ.12.0690 (□), LinJ.06.0470 (○) and LinJ.06.0480 (●). Lanes: 1, <i>L</i>. <i>infantum</i> WT; 2, <i>MRE11</i>^<i>-/-</i> and 3, <i>MRE11</i>^<i>-/-</i><i>RAD50</i>^<i>-/-</i>. <b>(C, D)</b> Reads were mapped to the <i>L</i>. <i>infantum</i> JPCM5 genome and log₂-transformed normalized read counts for non-overlapping 5 kb genomic windows are shown for chromosomes 12 and 06. Chromosome 12 is triploid in our <i>L</i>. <i>infantum</i> JPCM5 WT strain and a log₂ fold change of 0,5 would suggest that chromosome 12 is tetraploid in <i>L</i>. <i>infantum</i> 263 WT. Arrows indicate direction and breakpoints of the translocations. Blue, <i>L</i>. <i>infantum</i> 263 WT; orange, <i>LiMRE11</i>^<i>-/-</i> and green, <i>LiMRE11</i>^<i>-/-</i><i>RAD50</i>^<i>-/-</i>.</p

Translocation between chromosome 08 and 17 in <i>L</i>. <i>infantum MRE11</i>^<i>-/-</i><i>RAD50</i>^<i>-/-</i> cells.

<p><b>(A)</b> Schematic representation of the translocation T 08–17 between chromosomes 08 and 17. <b>(B)</b> <i>L</i>. <i>infantum</i> chromosomes were separated by pulsed-field gel electrophoresis, transferred on membranes then hybridized with probes from LinJ.08.0280 (□), LinJ.08.0290 (■), LinJ.17.1130 (○) and LinJ.17.1140 (●). Lanes: 1, <i>L</i>. <i>infantum</i> WT; 2, <i>MRE11</i>^<i>-/-</i> and 3, <i>MRE11</i>^<i>-/-</i><i>RAD50</i>^<i>-/-</i>. <b>(C, D)</b> Log₂-transformed normalized read counts for non-overlapping 5 kb genomic windows on chromosomes 08 and 17. The Y-axis indicates log₂ fold change from an initial diploid state for chromosomes 08 and 17. Arrows indicate direction and breakpoints of the translocations. Blue, <i>L</i>. <i>infantum</i> 263 WT; orange, <i>LiMRE11</i>^<i>-/-</i> and green, <i>LiMRE11</i>^<i>-/-</i><i>RAD50</i>^<i>-/-</i>.</p

Gene amplification and rearrangement in <i>L</i>.<i>infantum MRE11</i>^<i>-/-</i><i>RAD50</i>^<i>-/-</i> cells selected for methotrexate (MTX) resistant cells.

<p><b>(A, D)</b><i>L</i>. <i>infantum</i> WT clone (lane +), <b>(B, E)</b> <i>L</i>. <i>infantum MRE11</i>^<i>-/-</i> clone (lane +) and <b>(C, F)</b> <i>L</i>. <i>infantum MRE11</i>^<i>-/-</i><i>RAD50</i>^<i>-/-</i> clones (lanes 1–10) were selected for MTX resistance up to 1600nM MTX, their chromosomes were separated by pulsed-field gel electrophoresis using a separation range between 150kb and 1500kb, transferred on membranes then hybridized with <i>PTR1</i> <b>(A, B, C)</b> and <i>DHFR-TS</i> <b>(D, E, F)</b> probes. Lanes 0 and lanes—are parasites without drug selection.</p

Reduction of mapped read counts at subtelomeric locations in <i>L</i>. <i>infantum MRE11</i>^<i>-/-</i><i>RAD50</i>^<i>-/-</i> cells.

<p>Log₂-transformed normalized read counts on chromosome 05 <b>(A)</b>, chromosome 28 <b>(B)</b> and chromosome 34 <b>(C)</b>. Blue, <i>L</i>. <i>infantum</i> 263 WT; orange, <i>LiMRE11</i>^<i>-/-</i> and green, <i>LiMRE11</i>^<i>-/-</i><i>RAD50</i>^<i>-/-</i>. Black arrows define the location of probes derived from genes <i>LinJ</i>.<i>05</i>.<i>0060</i>, <i>LinJ</i>.<i>28</i>.<i>0050</i> and <i>LinJ</i>.<i>34</i>.<i>0070</i>. White arrows define the location of probes derived from control genes <i>LinJ</i>.<i>05</i>.<i>0560</i>, <i>LinJ</i>.<i>28</i>.<i>1020</i> and <i>LinJ</i>.<i>34</i>.<i>1480</i> that were used for Southern blot hybridization of PvuII-digested genomic DNAs. Lanes: 1, <i>L</i>. <i>infantum</i> WT; 2, <i>LiMRE11</i>^<i>-/-</i> and 3, <i>LiMRE11</i>^<i>-/-</i><i>RAD50</i>^<i>-/-</i>.</p

Translocation events in <i>L</i>. <i>infantum MRE11</i> and <i>RAD50</i> null mutants.

<p><b>(A, C, E)</b> Schematic representation of the translocations T 12–17 <b>(A)</b>, T 12–18 <b>(C)</b> and T 18–20 <b>(E)</b>. <b>(B, D, F)</b> <i>L</i>. <i>infantum</i> chromosomes were separated by pulsed-field gel electrophoresis, transferred on membranes then hybridized with probes from LinJ.17.1180 (■), LinJ.18.1520 (●), LinJ.20.1570 (▲). Lanes: 1, <i>L</i>. <i>infantum</i> WT; 2, <i>MRE11</i>^<i>-/-</i> and 3, <i>MRE11</i>^<i>-/-</i><i>RAD50</i>^<i>-/-</i>.</p

Chromosomal Translocations in the Parasite <i>Leishmania</i> by a MRE11/RAD50-Independent Microhomology-Mediated End Joining Mechanism

<div><p>The parasite <i>Leishmania</i> often relies on gene rearrangements to survive stressful environments. However, safeguarding a minimum level of genome integrity is important for cell survival. We hypothesized that maintenance of genomic integrity in <i>Leishmania</i> would imply a leading role of the MRE11 and RAD50 proteins considering their role in DNA repair, chromosomal organization and protection of chromosomes ends in other organisms. Attempts to generate <i>RAD50</i> null mutants in a wild-type background failed and we provide evidence that this gene is essential. Remarkably, inactivation of <i>RAD50</i> was possible in a <i>MRE11</i> null mutant that we had previously generated, providing good evidence that <i>RAD50</i> may be dispensable in the absence of <i>MRE11</i>. Inactivation of the <i>MRE11</i> and <i>RAD50</i> genes led to a decreased frequency of homologous recombination and analysis of the null mutants by whole genome sequencing revealed several chromosomal translocations. Sequencing of the junction between translocated chromosomes highlighted microhomology sequences at the level of breakpoint regions. Sequencing data also showed a decreased coverage at subtelomeric locations in many chromosomes in the <i>MRE11</i>^<i>-/-</i><i>RAD50</i>^<i>-/-</i> parasites. This study demonstrates an MRE11-independent microhomology-mediated end-joining mechanism and a prominent role for MRE11 and RAD50 in the maintenance of genomic integrity. Moreover, we suggest the possible involvement of RAD50 in subtelomeric regions stability.</p></div

Sequence microhomology at translocation breakpoints.

<p>DNA sequences obtained from direct sequencing of the junctions T 12–06 <b>(A)</b>, T 12–18 <b>(B)</b>, T 18–20 <b>(C)</b> and T 08–17 <b>(D)</b>. Sequences of wild-type parasites found close to breakpoints were aligned to the respective chromosomes involved as well as the resulting hybrid chromosome. Microhomology sequences are highlighted in capital bold letters. Asterisk indicates that the sequence corresponds to the antisense strand.</p