Comparison of body condition scores (BCS) and the number of gestational females in each experimental group which consisted of crossbred heifers inseminated with cryopreserved sperm with (treatment) or without (control) recombinant heparin binding proteins.

*<p>Estimated values with the logistic model (a 95% confidence interval).</p

Visualization of representative control and treatment sperm samples included the following: a) fertility associated antigen (FAA) antibody control sample (FAA negative) without FAA present on sperm (provided by Midland Bioproducts Corporation®) and verified after the application of fluorescein conjugated FAA antibody, b) FAA protein control sample (FAA negative, provided by Midland Bioproducts Corporation®) with fluorescein conjugated FAA recombinant protein added to sperm, c) fluorescein conjugated Type-2 tissue inhibitor of metalloproteinase (TIMP-2) antibody added to sperm, and d) fluorescein conjugated TIMP-2 protein added to sperm.

<p>Visualization of representative control and treatment sperm samples included the following: a) fertility associated antigen (FAA) antibody control sample (FAA negative) without FAA present on sperm (provided by Midland Bioproducts Corporation®) and verified after the application of fluorescein conjugated FAA antibody, b) FAA protein control sample (FAA negative, provided by Midland Bioproducts Corporation®) with fluorescein conjugated FAA recombinant protein added to sperm, c) fluorescein conjugated Type-2 tissue inhibitor of metalloproteinase (TIMP-2) antibody added to sperm, and d) fluorescein conjugated TIMP-2 protein added to sperm.</p

Percentages of controlled internal drug release (CIDR) devices retained, presentation of estrus and gestations summarized for the different groups.

<p>Group A (Control) = crossbred heifers inseminated with cryopreserved, frozen-thawed sperm without added recombinant heparin binding proteins (<i>r</i>HBPs), i.e., control group. Group B (<i>r</i>HBPs) = crossbred heifers inseminated with cryopreserved, frozen-thawed sperm with added <i>r</i>HBPs, i.e., treatment group.</p

Fitted model of the logistic regression analysis of the pregnancy rates for estimating the percentages of gestations under the different experimental conditions: .

<p>Null deviance: 30.63 on 3 degrees of freedom.</p><p>Residual deviance: 1.5 on 1 degree of freedom.</p><p>Akaike Information Criterion (AIC): 17.8.</p><p><i>r</i>HBPs = recombinant heparin binding proteins, 0 or 1 for the treatment or control group, respectively.</p><p>BCS = body condition score, 0 or 1 for BCS = 4 or 6, respectively.</p