Table_1_Lack of associations of microRNAs with severe NAFLD in people living with HIV: discovery case-control study.xlsx

Background & objectiveNonalcoholic fatty liver disease (NAFLD) is highly prevalent in people living with HIV (PLWH) and the expression of some microRNAs could be useful as biomarkers for the diagnosis of NAFLD. The aim of this study was to identify patterns of differential expression of microRNAs in PLWH and assess their diagnostic value for NALFD.MethodsA discovery case-control study with PLWH was carried out. The expression of miRNAs was determined using HTG EdgeSeq technology. Cases were defined as patients with severe NAFLD and controls as patients without NAFLD, characterized using the controlled attenuation parameter (CAP). Cases and controls were matched 1:1 for age, sex, BMI, CD4+ lymphocyte count, active HCV infection, and ART regimen.ResultsSerum 2,083 simultaneous microRNA transcripts were analyzed using HTG technology and compared between cases and controls. Forty-five patients, 23 cases, and 22 controls were included in the study. In the analysis of the expression pattern of the 2,083 microRNAs, no differential expression patterns were found between both groups of patients included in the study.ConclusionAnalysis of the microRNA transcriptome profile of nonobese PLWH with severe NAFLD did not appear to differ from that of patients without NAFLD. Thus, microRNA might not serve as a proper biomarker for predicting severe NALFD in this population.</p

DataSheet_1_Lack of associations of microRNAs with severe NAFLD in people living with HIV: discovery case-control study.pdf

Background & objectiveNonalcoholic fatty liver disease (NAFLD) is highly prevalent in people living with HIV (PLWH) and the expression of some microRNAs could be useful as biomarkers for the diagnosis of NAFLD. The aim of this study was to identify patterns of differential expression of microRNAs in PLWH and assess their diagnostic value for NALFD.MethodsA discovery case-control study with PLWH was carried out. The expression of miRNAs was determined using HTG EdgeSeq technology. Cases were defined as patients with severe NAFLD and controls as patients without NAFLD, characterized using the controlled attenuation parameter (CAP). Cases and controls were matched 1:1 for age, sex, BMI, CD4+ lymphocyte count, active HCV infection, and ART regimen.ResultsSerum 2,083 simultaneous microRNA transcripts were analyzed using HTG technology and compared between cases and controls. Forty-five patients, 23 cases, and 22 controls were included in the study. In the analysis of the expression pattern of the 2,083 microRNAs, no differential expression patterns were found between both groups of patients included in the study.ConclusionAnalysis of the microRNA transcriptome profile of nonobese PLWH with severe NAFLD did not appear to differ from that of patients without NAFLD. Thus, microRNA might not serve as a proper biomarker for predicting severe NALFD in this population.</p

Representative photomicrographs of liver sections from 3 wild boar naturally infected with hepatitis E virus.

<p>Fig 2A: liver with absence of association of the HEV antigen inside the lymphoplasmacytic aggregates (Immunohistochemistry (IHC) with the avidin-biotin-peroxidase complex (ABC) method counterstained with hematoxylin). Fig 2B: hepatitis E virus (HEV) immunolabeling in the cytoplasm of the Kupffer cells (arrowhead) and the sinusoidal endothelial cells (arrow) (IHC with ABC method). Fig 2C: HEV immunostaining of a light lymphoplasmacytic infiltrate (arrowhead) (IHC with ABC method). Fig 2D: liver showing slight labeling for HEV antigen in the hepatic parenchyma, which is appreciable in the Kupffer cells (inset) (IHC with ABC method).</p

Main characteristics of the study population 1 and the subpopulation where liver stiffness progression was evaluated.

<p>Main characteristics of the study population 1 and the subpopulation where liver stiffness progression was evaluated.</p

Liver stiffness progression rate according to <i>PNPLA3</i> rs738409 genotype.

<p>Liver stiffness progression rate according to <i>PNPLA3</i> rs738409 genotype.</p

Histopathologic lesions in wild boar naturally infected with hepatitis E virus.

<p>Fig 1A: liver with presence of mild intralobular lymphoplasmacytic aggregates in the hepatic parenchyma. Inset showing a lymphoplasmacytic aggregate at higher magnification (hematoxylin and eosin, HE). Fig 1B: liver showing slight perilobular infiltrations of lymphocytes (arrowheads) (HE). Fig 1C: hepatic lobule with moderate hyperemia of liver sinusoids (HE).</p

Associations with significant liver stiffness progression (Study 2).

<p>Associations with significant liver stiffness progression (Study 2).</p