The humoral immune response of hPBMC-HUMAMICE after immunization with HBsAg vaccine.

<p>(A) The HBsAg specific IgG and (B) HBsAg specific IgM responses of immunized hPBMC-HUMAMICE before (0 w) and every two weeks after immunization (before each immunization boost).</p

Flow cytometry analysis of immuno-deficient HUMAMICE.

<p>Comparative cell analysis by flow cytometry between immuno-deficient HUMAMICE (HLA-A2^+/+/DR1^+/+/H-2-β₂m^-/-/IAβ^-/-/Rag2^-/-/IL2rγ^-/-/Perf^-/-) and immuno-competent C57BL/6 mice. The left panel shows the analysis of immuno-deficient HUMAMICE and the right panel was the immuno-competent C57BL/6 mice. (A) Samples in Fig 1A were double-labelled with mCD3-PE and mCD19-FITC. (B) Samples in Fig 1B were labelled with mCD4-APC and mCD8-PE-Cy5.</p

The experimental procedure of hPBMC transplantation and immunization with HBsAg vaccine in HUMAMICE.

<p>The experimental procedure of hPBMC transplantation and immunization with HBsAg vaccine in HUMAMICE.</p

Flow cytometry analysis after five and eight weeks of hPBMC transplantation in HUMAMICE.

<p>Flow cytometry analysis was done at five, eight weeks and three months after the hPBMC were transferred. Results of two representative mice (from nine analyzed mice) which were analyzed at five and eight weeks after transplantation are shown in Fig 5. (A) For the mouse which was analyzed at 5 weeks after transplantation, 11.6% of splenocytes were composed by hCD45⁺ cells, with 45.2% of this population being hCD19⁺, and 17.1% being hCD45⁺hCD3⁺ T lymphocytes: 29.0% of them were hCD3⁺CD8⁺ and 60.9% were hCD3⁺CD4⁺ T cells. (B) For the mouse which was analyzed at 8 weeks after transplantation, 9.7% of splenocytes were composed by hCD45⁺ cells, with 49.4% of this population being hCD19⁺, and 16.1% being T lymphocytes: 30.0% of them were hCD3⁺CD8⁺ and 59.0% were hCD3⁺CD4⁺ T lymphocytes.</p

The tumor growth kinetics in HUMAMICE after transfer of RAMOS specific splenocytes from parental Sure-L1 mice.

<p>(A) Tumor growth kinetics following transfer of tumor specific splenocytes from Sure-L1 mice in three immuno-deficient HUMAMICE bearing a solid RAMOS tumor (mouse No.926; 948; 952) and the control mouse No.953 without the transfer of tumor-bearing cells. (B) Tumor growth kinetics following transfer of purified tumor specific CD8⁺ T lymphocytes from Sure-L1 mice in two immuno-deficient HUMAMICE bearing a solid RAMOS tumor (mouse No.208 ; 326) and the control mouse No.323 without transfer of tumor-bearing cells. (C) Tumor growth kinetics following transfer of tumor specific splenocytes depleted of CD8⁺ T cells (purified CD8^- T lymphocytes) from Sure-L1 mice in three immuno-deficient HUMAMICE bearing a solid RAMOS tumor (mouse No.139; 212; 337) and the control mouse No.323 without transfer of tumor-bearing cells.</p

The evaluation of body weight of HUMAMICE after hPBMC transplantation.

<p>The average weight is shown as a percentage of starting weight. None of the HUMAMICE developed weight loss after hPBMC transplantation.</p

Summary of comparative cell analysis was implemented by flow cytometry between immuno-deficient HUMAMICE and immuno-competent C57BL/6 mice.

<p>Summary of comparative cell analysis was implemented by flow cytometry between immuno-deficient HUMAMICE and immuno-competent C57BL/6 mice.</p

The tumor growth kinetics in immuno-deficient HUMAMICE and immuno-competent Sure-L1 mice after subcutaneous injection of RAMOS tumor cells.

<p>(A) Comparative tumor growth in six immuno-deficient HUMAMICE (HLA-A2^+/+/DR1^+/+/H-2-β₂m^-/-/IAβ^-/-/Rag2^-/-/IL2rγ^-/-/Perf^-/-) and five immuno-competent Sure-L1 mice (HLA-A2^+/+/DR1^+/+/H-2-β₂m^-/-/IAβ^-/-) 25 days after the subcutaneous injection of RAMOS cells. Fig 2A (left) and B (left) show HUMAMICE; while Fig 2A (right) and B (right) show Sure-L1 mice.</p

Summary of all the HUMAMICE transplanted with HLA-A2⁺/DR1⁺ hPBMCs.

<p>Summary of all the HUMAMICE transplanted with HLA-A2⁺/DR1⁺ hPBMCs.</p

Characteristics of patients & IHC markers (univariate analysis).

<p><b>Legends</b>: pts = patients sbtype = subtype L = lymphocytes MC = mast cells nber = number IHC:immunohistochemical CV: continuous variable HRS: Hodgkin and Reed Sternberg cells.</p