Second derivatives of IR spectra.

<p>Spectra recorded on steatosis or non-steatotic hepatocytes were superimposed (upper panel). Second derivatives of the spectra were calculated and superimposed in the frequency domain 2600–3200 cm⁻¹ (lower panel).</p

Spectroscopic analysis of non-steatotic hepatocytes on fatty liver.

<p>Spectroscopic analyses were performed on periportal hepatocytes on tissue section from normal or fatty liver. The video image is shown (left panel) with the corresponding averaged IR spectra (right panel) and the chemical imaging of the sum of DAG (middle panel).</p

History of patients and origin of samples.

<p>History of patients and origin of samples.</p

Analysis of steatosis using synchrotron FTIR microspectroscopy.

<p>A) Optical image of steatotic hepatocytes containing steatotic vesicles (white star) and non-steatotic hepatocytes (black star). B) Averaged IR spectra recorded inside steatotic vesicles (upper spectrum in blue) or on non-steatotic hepatocytes (lower spectrum in red). The band corresponding to olefin (3000–3060 cm⁻¹) is labelled by a black arrow. C) Chemical imaging of some bands on the tissue section.</p

Assignment of frequency to chemical functions.

<p>From <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007408#pone.0007408-Dreissig1" target="_blank">[19]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007408#pone.0007408-Banyay1" target="_blank">[20]</a>.</p

Histological features of steatosis.

<p>Tissue sections of 6 µm thickness were performed on paraffin embedded biopsies from normal liver or from fatty liver and stained with HES (hematoxylin, eosin and safran). Normal hepatic lobule without steatosis (left panel) or fatty liver area exhibiting macrovacuolar and microvesicular steatosis (right panel) are shown. Upper panel: ×100, lower panel: ×400. PT: portal tract, BD: biliary duct, PV: portal vein, HA: hepatic artery, CLV: centrilobular vein, SV: steatotic vacuole.</p