37 research outputs found
Contribution of Fluorescence Techniques in Determining the Efficiency of the Non-thermal Plasma Treatment
We have recently developed a non-thermal plasma (NTP) equipment intended to sterilize fragile medical devices and maintain the sterile state of items downstream the treatment. With traditional counts on agar plate a six log reduction of Staphylococcus aureus viability was obtained within 120 min of O2, Ar, or N2 NTP treatments. However to determine the best NTP process, we studied the different physiological states of S. aureus by flow cytometry (FC) and confocal laser scanning microscopy (CLSM) focusing on the esterasic activity and membrane integrity of the bacteria. Two fluorochromes, 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate and propidium iodide were used in order to distinguish three sub-populations: metabolically active, permeabilized, and damaged bacteria that can be in the viable but nonculturable state. FC and CLSM highlight that O2 and Ar NTP treatments were the most attractive processes. Indeed, a 5 min of Ar NTP generated a high destruction of the structure of bacteria and a 120 min of O2 NTP treatment led to the higher decrease of the total damaged bacteria population. SEM observations showed that in presence of clusters, bacteria of upper layers are easily altered compared to bacteria in the deeper layers. In conclusion, the plate counting method is not sufficient by itself to determine the best NTP treatment. FC and CLSM represent attractive indicator techniques to select the most efficient gas NTP treatment generating the lowest proportion of viable bacteria and the most debris
Réhabilitation prothétique de l'enfant
REIMS-BU Santé (514542104) / SudocPARIS-BIUM (751062103) / SudocLILLE2-UFR Odontologie (593502202) / SudocSudocFranceF
Contamination au cabinet dentaire : incidence, risques et prévention
REIMS-BU Santé (514542104) / SudocSudocFranceF
Prise en charge Odontologique de deux enfants atteints de dysplasie ectodermique anhydrotique
REIMS-BU Santé (514542104) / SudocSudocFranceF
Les différentes sources d'apport en fluorures chez l'enfant
LILLE2-UFR Odontologie (593502202) / SudocREIMS-BU Santé (514542104) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF
"Une dent contre la maltraitance" (le rôle du chirurgien dentiste dans le dépistage et la prévention de la matraitance)
REIMS-BU Santé (514542104) / SudocPARIS-BIUM (751062103) / SudocLILLE2-UFR Odontologie (593502202) / SudocSudocFranceF
Dens in dente
LILLE2-UFR Odontologie (593502202) / SudocREIMS-BU Santé (514542104) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF
Amélogenèse imparfaite (du diagnostic à la prise en charge pluridisciplinaire)
REIMS-BU Santé (514542104) / SudocPARIS-BIUM (751062103) / SudocLILLE2-UFR Odontologie (593502202) / SudocSudocFranceF
Microdélétion 22q11 (prise en charge de deux enfants atteints du syndrome de Di George)
REIMS-BU Santé (514542104) / SudocPARIS-BIUM (751062103) / SudocLILLE2-UFR Odontologie (593502202) / SudocSudocFranceF
Amélogenèse imparfaite (du diagnostic à la prise en charge pluridisciplinaire)
REIMS-BU Santé (514542104) / SudocPARIS-BIUM (751062103) / SudocLILLE2-UFR Odontologie (593502202) / SudocSudocFranceF