2 research outputs found
New 2-in-1 Polyelectrolyte Step-by-Step Film Buildup without Solution Alternation: From PEDOT-PSS to Polyelectrolyte Complexes
Although never emphasized and increasingly used in organic electronics,
PEDOT-PSS (poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonate))
layer-by-layer (lbl) film construction violates the alternation of
polyanion and polycation rule stated as a prerequisit for a step-by-step
film buildup. To demonstrate that this alternation is not always necessary,
we studied the step-by-step construction of films using a single solution
containing polycation/polyanion complexes. We investigated four different
systems: PEDOT-PSS, bPEI-PSS (branched poly(ethylene imine)-poly(sodium
4-styrene sulfonate)), PDADMA-PSS (poly(diallyl dimethyl ammonium)-PSS),
and PAH-PSS (poly(allylamine hydrochloride)-PSS). The film buildup
obtained by spin-coating or dipping-and-drying process was monitored
by ellipsometry, UV–vis-NIR spectrophotometry, and quartz-crystal
microbalance. The surface morphology of the films was characterized
by atomic force microscopy in tapping mode. After an initial transient
regime, the different films have a linear buildup with the number
of deposition steps. It appears that, when the particles composed
of polyanion-polycation complex and complex aggregates in solution
are more or less liquid (case of PEDOT-PSS and bPEI-PSS), our method
leads to smooth films (roughness on the order of 1–2 nm). On
the other hand, when these complexes are more or less solid particles
(case of PDADMA-PSS and PAH-PSS), the resulting films are much rougher
(typically 10 nm). Polycation/polyanion molar ratios in monomer unit
of the liquid, rinsing, and drying steps are key parameters governing
the film buildup process with an optimal polycation/polyanion molar
ratio leading to the fastest film growth. This new and general lbl
method, designated as <i>2-in-1 method</i>, allows obtaining
regular and controlled film buildup with a single liquid containing
polyelectrolyte complexes and opens a new route for surface functionalization
with polyelectrolytes
Biomimetic Cryptic Site Surfaces for Reversible Chemo- and Cyto-Mechanoresponsive Substrates
Chemo-mechanotransduction, the way by which mechanical forces are transformed into chemical signals, plays a fundamental role in many biological processes. The first step of mechanotransduction often relies on exposure, under stretching, of cryptic sites buried in adhesion proteins. Likewise, here we report the first example of synthetic surfaces allowing for specific and fully reversible adhesion of proteins or cells promoted by mechanical action. Silicone sheets are first plasma treated and then functionalized by grafting sequentially under stretching poly(ethylene glycol) (PEG) chains and biotin or arginine-glycine-aspartic acid (RGD) peptides. At unstretched position, these ligands are not accessible for their receptors. Under a mechanical deformation, the surface becomes specifically interactive to streptavidin, biotin antibodies, or adherent for cells, the interactions both for proteins and cells being fully reversible by stretching/unstretching, revealing a reversible exposure process of the ligands. By varying the degree of stretching, the amount of interacting proteins can be varied continuously